Journal of Natural Medicines

, Volume 69, Issue 3, pp 267–277 | Cite as

In vitro and in vivo antimutagenic effects of DIG, a herbal preparation of Berberis vulgaris, Taraxacum officinale and Arctium lappa, against mitomycin C

  • C. Di Giorgio
  • L. Boyer
  • M. De Meo
  • C. Laurant
  • R. Elias
  • E. Ollivier
Original Paper


DIG, a liquid herbal preparation made from a mixture of diluted mother tinctures of Berberis vulgaris, Taraxacum officinale and Arctium lappa, was assessed for its antimutagenic properties against mitomycin C. The micronucleus assay on Chinese hamster ovary (CHO)-K1 cells was used to evaluate the in vitro anticlastogenic activity of DIG compared to those of separately diluted mother tinctures. The micronucleus assay was performed on mouse erythrocytes and the comet assay was performed on mouse liver, kidney, lung, brain and testicles to assess the protective effects of DIG (0.2 and 2 % at libitum) against an intraperitoneal injection of mitomycin C (1 mg Kg−1) in mice. DIG exerted a powerful anticlastogenic activity, under both pretreatment and simultaneous treatment conditions as assessed by the micronucleus assay in CHO-K1 cells. Its protective activity was greater than that observed for each mother tincture. DIG reduced micronuclei levels in mouse erythrocytes and suppressed >80 % of DNA strand breaks in the liver, kidney, lung, brain and testicles of mice exposed to mitomycin C.


Berberis vulgaris Taraxacum dens leonis officinale Arctium lappa major Antimutagenic Anticlastogenic Mice 



This work was supported financially by Sevene Pharma Laboratories (Monoblet, France).

Conflict of interest

The authors declare that there are no conflicts of interest.


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2015

Authors and Affiliations

  • C. Di Giorgio
    • 1
  • L. Boyer
    • 2
  • M. De Meo
    • 1
  • C. Laurant
    • 2
  • R. Elias
    • 3
  • E. Ollivier
    • 3
  1. 1.Laboratoire de Mutagenèse Environnementale, IMBE, IFR ECCOREV, Faculté de PharmacieAix-Marseille UnivMarseille Cedex 05France
  2. 2.SEVENE-PHARMAMonobletFrance
  3. 3.Laboratoire de Pharmacognosie et Ethnopharmacologie, UMR MD3, Faculté de PharmacieAix-Marseille UniversitéMarseille Cedex 05France

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