Journal of Natural Medicines

, Volume 69, Issue 1, pp 123–129 | Cite as

Screening of β-secretase and acetylcholinesterase inhibitors from plant resources

  • Kazuya Murata
  • Shinichi Matsumura
  • Yuri Yoshioka
  • Yoshihiro Ueno
  • Hideaki MatsudaEmail author


The therapeutic agents for dementia are limited due to the complex system underlying the mechanisms. Taking a preventive point of view, we focused on the inhibition of β-secretase and acetylcholinesterase (AChE). In addition, plant resources including herbs and spices have been widely consumed, and further, may be consumed for a long period over a lifetime. Considering this background, we screened β-secretase and AChE inhibitors from curry spices. Amongst them, curry leaf, black pepper, and turmeric extracts were effective to inhibit β-secretase. Furthermore, black pepper and turmeric extracts were also effective to inhibit AChE. Having these results in hand, we focused on the investigation of β-secretase inhibitors since the inhibitor of this enzyme has not previously been well investigated. As a result, α- and β-caryophyllene, β-caryophyllene oxide (from curry leaf), piperine (from black pepper), curcumin, demethoxycurcumin, and bisdemethoxycurcumin (from turmeric) were successfully identified as low molecular inhibitors. This is the first report to determine α- and β-caryophyllene, β-caryophyllene oxide, and piperine as β-secretase inhibitors. These compounds may pass through the blood brain barrier since their molecular weights are relatively low.


Dementia β-secretase Black pepper Curry leaf Turmeric 


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2014

Authors and Affiliations

  • Kazuya Murata
    • 1
  • Shinichi Matsumura
    • 2
  • Yuri Yoshioka
    • 2
  • Yoshihiro Ueno
    • 1
  • Hideaki Matsuda
    • 1
    Email author
  1. 1.Faculty of PharmacyKinki UniversityHigashiosakaJapan
  2. 2.Inabata Koryo, Co., Ltd.YodogawaJapan

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