Journal of Natural Medicines

, Volume 67, Issue 3, pp 662–671 | Cite as

Polypharmacy (herbal and synthetic drug combination): a novel approach in the treatment of type-2 diabetes and its complications in rats

  • Rupinder Kaur
  • Muhammad Afzal
  • Imran Kazmi
  • Iqbal Ahamd
  • Zubair Ahmed
  • Babar Ali
  • Sayeed Ahmad
  • Firoz Anwar


The aim of present study was to evaluate the effect of aqueous leaf extract of Annona squamosa with Glipizide in a high fat diet and streptozocin-induced type-2 diabetes. Nine groups (n = 6) of male Sprague-Dawley rats were used for the study, with. Basal blood glucose, urine volume, and body weights were measured and the rate were kept on a high fat diet. After 15 days, streptozocin in sub-diabetic dose (35 mg/kg) was administered to the animals to induce diabetes. With 1 week of consistent hyperglycemia, treatment was initiated. Aqueous extract of Annona squamosa was administered orally at 350 mg/kg body weight alone and in combination with reduced and reducing dose combinations of Glipizide. Blood glucose, body weight, urine volume were measured every 10th day. The elevated blood-glucose level in diabetic rats was controlled better with combination therapy compared with the synthetic drug alone or the herbal stand-alone drug. All the results were statistically significant (P < 0.001). A combination of Annona squamosa along with Glipizide may be helpful in dose reduction of Glipizide up to 50 %, reducing the risk of the onset of insulin therapy.


Annona squamosa Glipizide Combination therapy Biochemical parameter 


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2012

Authors and Affiliations

  • Rupinder Kaur
    • 1
    • 5
  • Muhammad Afzal
    • 1
  • Imran Kazmi
    • 1
  • Iqbal Ahamd
    • 2
  • Zubair Ahmed
    • 3
  • Babar Ali
    • 4
  • Sayeed Ahmad
    • 4
  • Firoz Anwar
    • 1
  1. 1.Siddhartha Institute of PharmacyDehra DunIndia
  2. 2.Department of Pharmaceutics, Faculty of PharmacyJamia HamdardNew DelhiIndia
  3. 3.Sebai Institute of Health SciencesJeddahKingdom of Saudi Arabia
  4. 4.Department of Pharmacognosy, Faculty of PharmacyJamia HamdardNew DelhiIndia
  5. 5.Pacific University UdaipurIndia

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