Journal of Natural Medicines

, Volume 67, Issue 3, pp 554–561 | Cite as

Comparative study of three Plumbago L. species (Plumbaginaceae) by microscopy, UPLC-UV and HPTLC

  • Ahmed M. Galal
  • Vijayasankar Raman
  • Bharathi Avula
  • Yan-Hong Wang
  • Chidananda Swamy Rumalla
  • Aruna Dharmapriya Weerasooriya
  • Ikhlas A. Khan
Original Paper

Abstract

This paper presents a comparative study of anatomy of leaves, stems and roots of three species of Plumbago, namely P. auriculata Lam., P. indica L. and P. zeylanica L. by light microscopy. The paper also provides qualitative and quantitative analysis of the naphthoquinone, plumbagin—a major constituent present in these species—using UPLC-UV. Microscopic examinations revealed the presence of distinctive differences in the anatomical features of the leaf, stem and root of the three species, and these can thus be used for identification and authentication of these species. UPLC-UV analysis showed the highest concentration of plumbagin in the roots of P. zeylanica (1.62 % w/w) followed by the roots of P. indica (0.97 % w/w) and then P. auriculata (0.33–0.53 % w/w). In contrast, plumbagin was not detected in the stems and leaves of P. indica and in the leaves of P. auriculata, whereas very low concentrations (<0.02 % w/w) of plumbagin were detected in the stems and leaves of P. zeylanica and in the stems of P. auriculata. HPTLC fingerprints of the leaf and root of the three species exhibited distinguishable profiles, while those of the stems were undifferentiated.

Keywords

Plumbago Plumbaginaceae Anatomy Microscopy Plumbagin UPLC-UV HPTLC 

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2012

Authors and Affiliations

  • Ahmed M. Galal
    • 1
  • Vijayasankar Raman
    • 1
  • Bharathi Avula
    • 1
  • Yan-Hong Wang
    • 1
  • Chidananda Swamy Rumalla
    • 1
  • Aruna Dharmapriya Weerasooriya
    • 1
  • Ikhlas A. Khan
    • 1
    • 2
  1. 1.National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of PharmacyUniversity of MississippiUniversityUSA
  2. 2.Department of Pharmacognosy, Research Institute of Pharmaceutical Sciences, School of PharmacyUniversity of MississippiUniversityUSA

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