Journal of Natural Medicines

, Volume 67, Issue 3, pp 571–575 | Cite as

Use of the potential DNA barcode ITS2 to identify herbal materials

  • Xiaohui Pang
  • Linchun Shi
  • Jingyuan Song
  • Xiaochen Chen
  • Shilin Chen
Original Paper

Abstract

A potential DNA barcode, ITS2, was studied to discriminate herbal materials to confirm their identities and ensure their safe application in pharmaceuticals. Here, a total of 4385 samples of 2431 species were collected, and these samples are from 61 commonly used herbs and their closely related species or adulterants. Based on assessments of the extent of genetic divergence, the DNA barcoding gap and the ability for species discrimination, our results suggest that ITS2 is a powerful tool for distinguishing herbs. For the first dataset including 61 herbs, ITS2 correctly identified 100 % of them. For the second dataset containing 51 herbs and their 2382 closely related species, ITS2 could discriminate correctly 48 herbs from their closely related species. For the third dataset comprising 34 herbs and their 111 adulterants, ITS2 could distinguish successfully all the herbs from their adulterants. In conclusion, the ITS2 region is an efficient marker for the authentication of herbal materials, and our study will accelerate the process of the application of the DNA barcoding technique in differentiating herbs.

Keywords

Herbal materials ITS2 DNA barcode Identification 

Notes

Acknowledgements

This study was supported by the Basic Scientific Research Operation Grants for State-Level Public Welfare Scientific Research Initiatives (YZ-12-08) and Beijing Municipal Science and Technology Foundation (D08080203640901).

Supplementary material

11418_2012_715_MOESM1_ESM.doc (820 kb)
Supplementary material 1 (DOC 820 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2012

Authors and Affiliations

  • Xiaohui Pang
    • 1
  • Linchun Shi
    • 1
  • Jingyuan Song
    • 1
  • Xiaochen Chen
    • 1
  • Shilin Chen
    • 1
  1. 1.Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingPeople’s Republic of China

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