Protective effect of Chresta martii extract against indomethacin-induced gastric lesions in mice
- 418 Downloads
Chresta martii (Asteraceae) is a plant found in the Xingó region (semi-arid area) in Northeastearn Brazil, and is recognized by the local population as a traditional herb used to treat gastric diseases. This is the first report of the chemical composition, acute toxicity, and gastroprotective effect in mice of the hydroalcoholic extract (HAE) from the aerial parts (leaves and flowers) of Chresta martii. Animals received HAE doses from 10 to 2000 mg/kg, i.p. or 50 to 3000 mg/kg, p.o.) and were observed over 48 h for toxicity signs and mortality; sub-chronic toxicity was evaluated through 14 days treatment with once-daily HAE doses (400 mg/kg, p.o.). The gastroprotective effect of HAE was demonstrated on the indomethacin-induced gastric ulcer model after the administration of extracts. Data comparison of ulcer index averages between saline and HAE (100 or 400 mg/kg, p.o.) groups showed significant (P < 0.01) inhibition (71.73 and 76.72 %, respectively) of indomethacin-induced gastric lesions. Histological analyses showed significant (P < 0.05) inhibition of leukocyte migration in HAE-treated groups. A fingerprint of the HAE obtained by HPLC/UV/MS analysis showed major peaks characteristic of sesquiterpene lactones. Compound 1 was isolated and elucidated as a new natural product. Its capacity to prevent leukocyte chemotaxis was demonstrated in vitro, corroborating the pharmacological effects observed for C. martii HAE.
KeywordsChresta martii Asteraceae Sesquiterpene lactones Gastric ulcer
This work was supported by Conselho Nacional de Desenvolvimento Científico e tecnológico (CNPq), Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), Programa Rede Nordeste de Biotecnologia (RENORBIO) and the Swiss National Science Foundation (Grant no. 200010-100083 to K. Hostettmann).
- 4.Robinson H (1980) Notes on the Lychnophorine genera Chresta and Eremanthus. Phytologia 45:89–100Google Scholar
- 5.Agra MF, Silva KN, Basilio IJLD, Freitas PF, Barbosa-Filho JM (2008) Survey of medicinal plants used in the region Northeast of Brazil. Braz J Pharmacog 18:472–508Google Scholar
- 9.Miller LC, Tainter ML (1944) Estimation of the ED50 and its error by means of logarithmic-probit graph paper. Proc Soc Exp Biol Med 57:261–264Google Scholar
- 11.Boyden S (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J Exp Med 15:433–466Google Scholar
- 16.Diniz MFFM, Medeiros IA, Santos HB, Oliveira KM, Vasconcelos THC, Aguiar FB, Toscano MG, Ribeiro EAN (2006) Haematological and biochemical parameter standardization of Swiss mice and Wistar rats. Rev Bras Ciências da Saúde 10(2):171–176Google Scholar
- 25.Lutnicki K, Szpringer E, Czerny K, Ledwozyw A (2001) Effects of ethanol and arachidonic acid pathway inhibitors on the effectiveness of gastric mucosa cytoprotection. Folia Morphol (Warsz) 60:47–56Google Scholar
- 27.Chattopadhyay I, Bandyopadhyay U, Biswas K, Maity P, Banerjee RK (2006) Indomethacin inactivates gastric peroxidase to induce reactive-oxygen-mediated gastric mucosal injury and curcumin protects it by preventing peroxidase inactivation and scavenging reactive oxygen. Free Radic Biol Med 40:1397–1408PubMedCrossRefGoogle Scholar
- 31.Dias LFT, Melo ES, Hernandes LS, Bacchi EM (2009) Antiulcerogenic and antioxidant activities of Baccharis trimera (Less) DC (Asteraceae). Braz J Pharmacogn 19:309–314Google Scholar
- 32.Silverio MS, Sousa OV, Del-Vechio-Vieira G, Miranda MA, Matheus FC, Kaplan MAC (2008) Pharmacological properties of the ethanol extract from Eremanthus erythropappus (DC.) McLeisch. Braz J Pharmacog 18:430–435Google Scholar