Tumor specific cytotoxicity of arctigenin isolated from herbal plant Arctium lappa L.
The effectiveness of cancer chemotherapy is often limited by the toxicity to other tissues in the body. Therefore, the identification of non-toxic chemotherapeutics from herbal medicines remains to be an attractive goal to advance cancer treatments. This study evaluated the cytotoxicity profiles of 364 herbal plant extracts, using various cancer and normal cell lines. The screening found occurrence of A549 (human lung adenocarcinoma) specific cytotoxicity in nine species of herbal plants, especially in the extract of Arctium lappa L. Moreover, purification of the selective cytotoxicity in the extract of Arctium lappa L. resulted in the identification of arctigenin as tumor specific agent that showed cytotoxicity to lung cancer (A549), liver cancer (HepG2) and stomach cancer (KATO III) cells, while no cytotoxicity to several normal cell lines. Arctigenin specifically inhibited the proliferation of cancer cells, which might consequently lead to the induction of apoptosis. In conclusion, this study found that arctigenin was one of cancer specific phytochemicals, and in part responsible for the tumor selective cytotoxicity of the herbal medicine.
KeywordsTumor Herbal Extract Cytotoxicity Arctigenin
We are grateful to the NMR center of Science Faculty, University of the Ryukyus, for the technical support to determinate the chemical structure of arctigenin.
- 2.Hoareau L, DaSilva EJ (1999) Medicinal plants: a re-emerging health aid. Electron J Biotechnol 2:56–70Google Scholar
- 5.Alphen JP, Aris A, Dharmananda S (1995) The book oriental medicine: an illustrated guide to the Asian arts of healing. Serindia Publications, LondonGoogle Scholar
- 7.Parkin DM, Muir CS, Whelan SL, Gao YT, Ferlay J, Powell J (2005) Cancer incidence in five continents, vol VIII. IARC Scientific Publication, LyonGoogle Scholar
- 10.Skeel RT (2003) Handbook of cancer chemotherapy. Lippincott Williams and Wilkins, PhiladelphiaGoogle Scholar
- 11.Camp-Sorrell D (2000) Chemotherapy: toxicity management. In: Yarbro CH, Frogge MH, Goodman M, Groenwald SL (eds) Cancer nursing: principles and practice, 5th edn. Jones and Bartlett Publishers, SudburyGoogle Scholar
- 12.Chabner B, Longo DL (2005) Cancer chemotherapy and biotherapy: principles and practice, 4th edn. Lippincott Williams & Wilkins, Philadelphia. ISBN 0781756286Google Scholar
- 16.Buchanan DR, White JD, O’Mara AM, Kalaghan JW, Smith WB, Minasian LM (2005) Research-design issues in cancer-symptom-management trials using complementary and alternative medicine: lesson from the National Cancer Institute Community Clinical Oncology Program experience. J Clin Oncol 23(27):6682–6689PubMedCrossRefGoogle Scholar
- 20.Park KI, Park HS, Kang SR, Nagappan A, Lee DH, Kim JA, Han DY, Kim GS (2011) Korean Scutellaria baicalensis water extract inhibits cell cycle G1/S transition by suppressing cyclin D1 expression and matrix-metalloproteinase-2 activity in human lung cancer cells. J Ethnopharmacol 133:634–641PubMedCrossRefGoogle Scholar