Journal of Natural Medicines

, Volume 66, Issue 2, pp 394–399 | Cite as

Hypoglycemic effects of clove (Syzygium aromaticum flower buds) on genetically diabetic KK-Ay mice and identification of the active ingredients

  • Minpei Kuroda
  • Yoshihiro Mimaki
  • Takayuki Ohtomo
  • Junji Yamada
  • Tozo Nishiyama
  • Tatsumasa Mae
  • Hideyuki Kishida
  • Teruo Kawada
Note

Abstract

Clove (Syzygium aromaticum flower buds) EtOH extract significantly suppressed an increase in blood glucose level in type 2 diabetic KK-Ay mice. In-vitro evaluation showed the extract had human peroxisome proliferator-activated receptor (PPAR)-γ ligand-binding activity in a GAL4-PPAR-γ chimera assay. Bioassay-guided fractionation of the EtOH extract resulted in the isolation of eight compounds, of which dehydrodieugenol (2) and dehydrodieugenol B (3) had potent PPAR-γ ligand-binding activities, whereas oleanolic acid (4), a major constituent in the EtOH extract, had moderate activity. Furthermore, 2 and 3 were shown to stimulate 3T3-L1 preadipocyte differentiation through PPAR-γ activation. These results indicate that clove has potential as a functional food ingredient for the prevention of type 2 diabetes and that 24 mainly contribute to its hypoglycemic effects via PPAR-γ activation.

Keywords

Clove Syzygium aromaticum PPAR-γ ligand-binding activity Neolignan Triterpenoid 

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2011

Authors and Affiliations

  • Minpei Kuroda
    • 1
  • Yoshihiro Mimaki
    • 1
  • Takayuki Ohtomo
    • 1
  • Junji Yamada
    • 1
  • Tozo Nishiyama
    • 2
  • Tatsumasa Mae
    • 2
  • Hideyuki Kishida
    • 2
  • Teruo Kawada
    • 3
  1. 1.School of PharmacyTokyo University of Pharmacy and Life SciencesHachiojiJapan
  2. 2.Frontier Biochemical and Medical Research LaboratoriesKaneka CorporationTakasagoJapan
  3. 3.Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of AgricultureKyoto UniversityUjiJapan

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