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Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography

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Abstract

In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine:water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.

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References

  1. Shen-Wei T (1970) Chinese herbal of Takwan (Daguan Bencao) Sung (Song Dynasty) compiled by Dr. Kimura and Dr. Masao Yosizaki with new index. Hirokawa Publishing Company, Tokyo, pp 182–183

  2. Committee of the Chinese Pharmocopeia (2005) The Chinese pharmacopoeia vol 1. Chemical Industry Press, Beijing, pp 223–224

  3. Pharmacopoeia Society of Japan (2006) The Japanese pharmacopoeia XV edn. Yakuji Nippo Ltd., Tokyo, pp 1283–1284

  4. Bensky D, Clavey S, Stöger E (2004) Chinese herbal medicine: Materia Medica, 3rd edn. Eastland Press, California, pp 3–6

    Google Scholar 

  5. Soni MG, Carabin IG, Griffiths JC, Burdock GA (2004) Safety of ephedra: lessons learned. Toxicol Lett 150:97–110. doi:10.1016/j.toxlet.2003.07.006

    Article  PubMed  CAS  Google Scholar 

  6. Liu YM, Sheu SJ, Chiou SH, Chang HC, Chen YP (1993) A comparative study of commercial samples of Ephedrae Herba. Planta Med 59:376–378. doi:10.1055/s-2006-959706

    Article  PubMed  CAS  Google Scholar 

  7. Cui J-F, Niu C-Q, Zhang J-S (1991) Determination of Ephedra alkaloids in Chinese Ephedra (Mahuang) by gas chromatography. Acta pharmaceutica Sinica 26:852–857

    PubMed  CAS  Google Scholar 

  8. Sagara K, Oshima T, Misaki T (1983) A simultaneous determination of norephedrine, pseudoephedrine, ephedrine and methylephedrine in Ephedrae Herba and oriental pharmaceutical preparations by ion-pair high-performance liquid chromatography. Chem Pharm Bull 31:2359–2365

    PubMed  CAS  Google Scholar 

  9. Zhang J-S, Tian Z, Lou Z-C (1988) Simultaneous determination of six alkaloids in Ephedrae Herba by high-performance liquid chromatography. Planta Med 54:69–70. doi:10.1055/s-2006-962342

    Article  CAS  Google Scholar 

  10. Moriyasu M, Endo M, Kanazawa R, Hashimoto Y, Kato A, Mizuno M (1984) High-performance liquid chromatographic determination of organic substances by metal chelate derivatization III. Analysis of Ephedra bases. Chem Pharm Bull 32:744–747

    CAS  Google Scholar 

  11. Lou Z-C, Zhang J-S, Li S-H, Tian Z, Qi Z (1995) Research on Mahuang. In: Lou Z-C, Qin B (eds) Species systematization and quality evaluation of commonly used Chinese traditional drugs (North-China Edition). United Publishers of Beijing Medical University and China Union Medical University, Beijing, pp 39–121

    Google Scholar 

  12. Cheng D-D, Guo P, Zhao J (1992) Studies of seasonal variation of alkaloids contained in Ephedra sinica Herba in Inner Mongolia. J China Pharm Uni 23:82–87

    CAS  Google Scholar 

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Acknowledgments

The work was financially supported by the National Outstanding Youth Funds of China (grant no. 30425018).

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Authors and Affiliations

  1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China

    Hao Hong, Dong-Hui Yang, Ming-Ying Shang, Xuan Wang & Shao-Qing Cai

  2. School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China

    Hu-Biao Chen

  3. Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan

    Masayuki Mikage

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  1. Hao Hong
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  2. Hu-Biao Chen
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  3. Dong-Hui Yang
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  4. Ming-Ying Shang
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  5. Xuan Wang
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  6. Shao-Qing Cai
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  7. Masayuki Mikage
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Correspondence to Shao-Qing Cai.

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Hong, H., Chen, HB., Yang, DH. et al. Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography. J Nat Med 65, 623–628 (2011). https://doi.org/10.1007/s11418-011-0528-8

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  • DOI: https://doi.org/10.1007/s11418-011-0528-8

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