Journal of Natural Medicines

, Volume 65, Issue 2, pp 370–374 | Cite as

Antifibrotic activity of coumarins from Cnidium monnieri fruits in HSC-T6 hepatic stellate cells

  • Eunjin Shin
  • Chul Lee
  • Sang Hyun Sung
  • Young Choong Kim
  • Bang Yeon Hwang
  • Mi Kyeong Lee
Note

Abstract

The CHCl3 fraction of Cnidium monnieri fruits significantly inhibited the proliferation of hepatic stellate cells in an in-vitro assay system employing HSC-T6 hepatic stellate cell lines. Activity-guided fractionation of the CHCl3 fraction of C. monnieri led to the isolation of ten coumarins: osthol (1), meranzin (2), auraptenol (3), meranzin hydrate (4), 7-hydroxy-8-methoxy coumarin (5), imperatorin (6), xanthotoxol (7), xanthotoxin (8), bergapten (9) and isopimpinellin (10). Of these, compounds 1 and 6 significantly inhibited proliferation of HSCs in a time- and concentration-dependent manner. In addition, compounds 1 and 6 significantly reduced collagen content in HSC-T6 cells.

Keywords

Cnidium monnieri Coumarin HSC-T6 Hepatic stellate cells Osthol Imperatorin 

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2010

Authors and Affiliations

  • Eunjin Shin
    • 1
  • Chul Lee
    • 1
  • Sang Hyun Sung
    • 2
  • Young Choong Kim
    • 2
  • Bang Yeon Hwang
    • 1
  • Mi Kyeong Lee
    • 1
  1. 1.College of PharmacyChungbuk National UniversityCheongjuKorea
  2. 2.College of Pharmacy and Research Institute of Pharmaceutical ScienceSeoul National UniversitySeoulKorea

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