Antifibrotic activity of coumarins from Cnidium monnieri fruits in HSC-T6 hepatic stellate cells
The CHCl3 fraction of Cnidium monnieri fruits significantly inhibited the proliferation of hepatic stellate cells in an in-vitro assay system employing HSC-T6 hepatic stellate cell lines. Activity-guided fractionation of the CHCl3 fraction of C. monnieri led to the isolation of ten coumarins: osthol (1), meranzin (2), auraptenol (3), meranzin hydrate (4), 7-hydroxy-8-methoxy coumarin (5), imperatorin (6), xanthotoxol (7), xanthotoxin (8), bergapten (9) and isopimpinellin (10). Of these, compounds 1 and 6 significantly inhibited proliferation of HSCs in a time- and concentration-dependent manner. In addition, compounds 1 and 6 significantly reduced collagen content in HSC-T6 cells.
KeywordsCnidium monnieri Coumarin HSC-T6 Hepatic stellate cells Osthol Imperatorin
We thank Prof. S.L. Freidman (Columbia University, New York) for the kindly gift of the HSC-T6 cells. This work was supported by the Medical Research Center Program (2010-0029480), the Regional Core Research Program (Chungbuk BIT Research-Oriented University Consortium) and Basic Science Research Program (2010-0025054) through the National Research Foundation of Korea (NRF).
- 9.Zhu YP (1999) Chinese material medica, chemistry, pharmacology and application. Harwood Academic Publishers, Amsterdam, pp 624–625Google Scholar
- 12.Ito C, Furukawa H (1987) Constituents of Murraya exotica L. Structure elucidation of new coumarins. Chem Pharm Bull 35:4277–4285Google Scholar
- 14.Imai F, Kinoshita T, Sankawa U (1989) Constituents of the leaves of Murraya paniculata collected in Taiwan. Chem Pharm Bull 37:358–362Google Scholar