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Journal of Natural Medicines

, Volume 65, Issue 1, pp 122–128 | Cite as

Antinociceptive activity of Hypericum grandifolium Choisy in mice

  • Celia Xiomara Bonkanka
  • Candelaria del Carmen Sánchez-Mateo
  • Rosa María Rabanal
Original Paper

Abstract

The present study investigated the antinociceptive activity of different extracts prepared from the aerial parts of blossom of Hypericum grandifolium Choisy—a species native to the Macaronesian Region—using the acetic acid-induced writhing, formalin and tail flick test in mice. Oral administration of methanol extract (500 and 1,000 mg/kg p.o.), the aqueous, butanol and chloroform fractions (500 mg/kg p.o.) as well as subfractions F2 and F3 (45 mg/kg p.o.) from the chloroform fraction significantly inhibited acetic acid-induced writhing, with values ranging from 28 to 50% of inhibition. The methanol extract (1,000 mg/kg p.o.) and chloroform fraction (500 mg/kg p.o.) significantly reduced both phases of formalin-induced pain (with inhibition values ranging from 18 to 53%), whereas subfraction F2 (45 mg/kg p.o.) significantly inhibited the late phase (30%). In the tail flick assay, only the chloroform fraction (500 mg/kg p.o.) significantly prolonged the tail flick response. Different constituents, such as flavonoids and benzophenone derivatives, could account for the effects observed. Taking together, the results indicate that Hypericum grandifolium Choisy possesses both peripheral and central antinociceptive activities in mice, suggesting an interesting therapeutic potential for this species in pain diseases.

Keywords

Hypericum grandifolium Choisy Antinociceptive activity Writhing test Formalin test Tail flick test Mice 

Notes

Acknowledgements

This work was supported in part by the Consejería de Educación, Cultura y Deportes del Gobierno de Canarias (PI2000/105) Project. We also thank La Laguna University, Caja Canarias, Spain, for providing a research grant to C.X.B.

References

  1. 1.
    Barnes J, Anderson LA, Phillipson JD (2001) St John’s wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties. J Pharm Pharmacol 53:583–600PubMedCrossRefGoogle Scholar
  2. 2.
    Bombardelli E, Morazzoni P (1995) Hypericum perforatum. Fitoterapia 66:43–68Google Scholar
  3. 3.
    Brattström A (2009) Long-term effects of St. John’s wort (Hypericum perforatum) treatment: a 1-year safety study in mid to moderate depression. Phytomedicine 16:277–283PubMedCrossRefGoogle Scholar
  4. 4.
    Kumar V (2006) Potential medicinal plants for CNS disorders: an overview. Phytother Res 20:1023–1035PubMedCrossRefGoogle Scholar
  5. 5.
    Galeotti N, Vivoli E, Bilia AN, Bergonzi MC, Bartolini A, Ghelardini A (2010) A prolonged protein kinase C-mediated, opioid-related antinociceptive effect of St. John’s wort in mice. J Pain 11:149–159PubMedCrossRefGoogle Scholar
  6. 6.
    Suntar IP, Akkol EK, Yilmazer D, Baykal T, Kirmizibekmez H, Alper M, Yesilada E (2010) Investigation on the in vivo wound healing potential of Hypericum perforatum L. J Ethnopharmacol 127:468–477PubMedCrossRefGoogle Scholar
  7. 7.
    Sánchez-Mateo CC, Bonkanka CX, Prado B, Rabanal RM (2007) Antidepressant activity of some Hypericum reflexum L. fil. extracts in the forced swimming test in mice. J Ethnopharmacol 112:115–121PubMedCrossRefGoogle Scholar
  8. 8.
    Sánchez-Mateo CC, Bonkanka CX, Rabanal RM (2009) Hypericum grandifolium Choisy: a species native to Macaronesian Region with antidepressant effect. J Ethnopharmacol 121:297–303PubMedCrossRefGoogle Scholar
  9. 9.
    Perazzo FF, Lima LM, Padilha MD, Rocha LM, Sousa PJC, Carvalho JCT (2008) Anti-inflammatory and analgesic activities of Hypericum brasiliense (Willd) standardized extract. Rev Bras Farmacogn 18:320–325Google Scholar
  10. 10.
    Sánchez-Mateo CC, Bonkanka CX, Hernández-Pérez M, Rabanal RM (2006) Evaluation of the analgesic and topical anti-inflammatory effects of Hypericum reflexum L. fil. J Ethnopharmacol 107:1–6PubMedCrossRefGoogle Scholar
  11. 11.
    Trovato A, Raneri E, Kouladis M, Tzakou O, Taviano MF, Galeti EM (2001) Anti-inflammatory and analgesic activity of Hypericum empetrifolium Willd. (Guttiferae). Farmaco 56:455–457PubMedCrossRefGoogle Scholar
  12. 12.
    Galati EM, Contarterse G, Miceli N, Taviano MF, Sdrafkakis V, Couladis M, Tzakou O, Lanuzza F (2008) Antiinflammatory and antioxidant activity of Hypericum rumeliacum Boiss. subsp. apollinis (Boiss. & Heldr.) Robson & Strid methanol extract. Phytother Res 22:766–771PubMedCrossRefGoogle Scholar
  13. 13.
    Rabanal RM, Arias A, Prado B, Hernández-Pérez M, Sánchez-Mateo CC (2002) Antimicrobial studies on three species of Hypericum from the Canary Islands. J Ethnopharmacol 81:287–292PubMedCrossRefGoogle Scholar
  14. 14.
    Wang N, Li P, Wang Y, Peng W, Wu Z, Tan S, Liang S, Shen X, Su W (2008) Hepatoprotective effect of Hypericum japonicum extract and its fractions. J Ethnopharmacol 116:1–6PubMedCrossRefGoogle Scholar
  15. 15.
    Mukherjee PK, Verpoorte R, Suresh B (2000) Evaluation of in vivo wound healing activity of Hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol 70:315–321PubMedCrossRefGoogle Scholar
  16. 16.
    Darias V, Bravo L, Barquín E, Martín-Herrera D, Fraile C (1986) Contribution to the ethnopharmacological study of the Canary Islands. J Ethnopharmacol 15:169–193PubMedCrossRefGoogle Scholar
  17. 17.
    Darias V, Bravo L, Rabanal R, Sánchez Mateo C, González Luis RM, Hernández Pérez AM (1989) New contribution to the ethnopharmacological study of the Canary Islands. J Ethnopharmacol 25:77–92PubMedCrossRefGoogle Scholar
  18. 18.
    Pérez de Paz PL, Hernández-Padrón CE (1999) Plantas medicinales o útiles en la flora canaria. Aplicaciones populares. Lemus, La LagunaGoogle Scholar
  19. 19.
    Prado B, Rabanal RM, Sánchez-Mateo CC (2002) Evaluation of the central properties of several Hypericum species from the Canary Islands. Phytother Res 16:740–744PubMedCrossRefGoogle Scholar
  20. 20.
    Sánchez-Mateo CC, Prado B, Rabanal RM (2002) Antidepressant effects of the methanol extract of several Hypericum species from the Canary Islands. J Ethnopharmacol 79:119–127PubMedCrossRefGoogle Scholar
  21. 21.
    Bonkanka CX, Smelcerovic A, Zuehlke S, Rabanal RM, Spiteller M, Sánchez-Mateo CC (2008) HPLC-MS analysis of anti-oedematogenic activity of Hypericum grandifolium Choisy (Hypericaceae). Planta Med 74:719–725PubMedCrossRefGoogle Scholar
  22. 22.
    Hunskaar S, Hole K (1987) The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain 30:103–114PubMedCrossRefGoogle Scholar
  23. 23.
    Tjolsen A, Bergev OG, Hunskaar S, Rosland JH, Hole K (1992) The formalin test: an evaluation of the method. Pain 51:5–17PubMedCrossRefGoogle Scholar
  24. 24.
    Tallarida RJ, Murray RB (1987) Manual of pharmacologic calculations with computer programs, 2nd edn. Springer, New YorkGoogle Scholar
  25. 25.
    Bighetti EJB, Hiruma-Lima CA, Gracioso JS, Souza Brito ARM (1999) Anti-inflammatory and antinociceptive effects in rodents of the essential oil of Croton cajucara Benth. J Pharm Pharmacol 51:1447–1453PubMedCrossRefGoogle Scholar
  26. 26.
    Collier HOJ, Dinneen LC, Johnson CA, Schneider C (1968) The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol 32:295–310Google Scholar
  27. 27.
    Santos ARS, Filho VC, Niero R, Viana AM, Moreno FN, Campos MM, Yunes RA, Calixto JB (1994) Analgesic effects of callus culture extracts from selected species of Phyllanthus in mice. J Pharm Pharmacol 46:755–759PubMedGoogle Scholar
  28. 28.
    Ramabadran K, Bansinath M, Turndorf H, Puig MM (1989) Tail immersion test for the evaluation of a nociceptive reaction in mice. Methodological considerations. J Pharmacol Methods 21:21–31PubMedCrossRefGoogle Scholar
  29. 29.
    Srinivasan K, Muruganandan S, Lal J, Chandra S, Tandan SK, Raviprakash V, Kumar D (2003) Antinoniceptive and antipyretic activities of Pongamia pinnata leaves. Phytother Res 17:259–264PubMedCrossRefGoogle Scholar
  30. 30.
    Apaydin S, Zeybek U, Ince I, Elgin G, Karamenderes C, Öztürk B, Tuglular I (1999) Hypericum triquetrifolium Turra. extract exhibits antinociceptive activity in the mouse. J Ethnopharmacol 67:307–312PubMedCrossRefGoogle Scholar
  31. 31.
    Viana AF, Heckler AP, Fenner R, Rates SMK (2003) Antinociceptive activity of Hypericum caprifoliatum and Hypericum polyantheum (Guttiferae). Braz J Med Biol Res 36:631–634PubMedGoogle Scholar
  32. 32.
    Rabanal RM, Bonkanka CX, Hernández-Pérez M, Sánchez-Mateo CC (2005) Analgesic and topical anti-inflammatory activity of Hypericum canariense L. and Hypericum glandulosum Ait. J Ethnopharmacol 96:591–596PubMedCrossRefGoogle Scholar
  33. 33.
    Calixto JB, Beirith A, Ferreira J, Santos ARS, Filho VC, Yunes RA (2000) Naturally occurring antinociceptive substances from plants. Phytother Res 14:401–418PubMedCrossRefGoogle Scholar
  34. 34.
    Gadotti VM, Schmeling LO, Machado C, Liz FH, Cechinel V, Meyre-Silva C, Santos ARS (2005) Antinociceptive action of the extract and the flavonoid quercitrin isolated from Bauhinia microstachya leaves. J Pharm Pharmacol 57:1345–1351PubMedCrossRefGoogle Scholar
  35. 35.
    Martínez AL, González-Trujano ME, Aguirre-Hernández E, Moreno J, Soto-Hernández M, López-Muñóz FJ (2009) Antinociceptive activity of Tilia americana var. mexicana inflorescences and quercetin in the formalin test and in an arthritic pain model in rats. Neuropharmacology 56:564–571PubMedCrossRefGoogle Scholar
  36. 36.
    Artini D, Buttinoni A, Dradi E, Logermann W, Mandelli V, Melloni P, Tommasini R, Tosolini G, Vita G (1971) Amides and amines with analgesic and antiinflammatory activity. Arzneimittel-Forsch 21:30–36Google Scholar
  37. 37.
    Matsuo T, Amemiya K, Saga K, Hamazaki Y, Tanaka M, Kato T (1981) Pharmacological and toxicological studies of a new non-steroidal anti-inflammatory drug: KC-8973. Arzneimittel-Forsh 31:1106–1110Google Scholar

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2010

Authors and Affiliations

  • Celia Xiomara Bonkanka
    • 1
  • Candelaria del Carmen Sánchez-Mateo
    • 1
  • Rosa María Rabanal
    • 1
  1. 1.Departamento de Medicina Física y Farmacología, Facultad de FarmaciaUniversidad de la LagunaLa LagunaSpain

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