Journal of Natural Medicines

, Volume 64, Issue 3, pp 383–387 | Cite as

Simultaneous determination of hydrolysable tannins in the petals of Rosa rugosa and allied plants

  • Sarangowa Ochir
  • ByoungJae Park
  • Makoto Nishizawa
  • Tsutomu Kanazawa
  • Minoru Funaki
  • Takashi YamagishiEmail author


The petals of Rosa rugosa and allied plants for medicinal use contain abundant hydrolysable tannins, and they show remarkable biological activities. The activities are dependent on the structures of the hydrolysable tannins, so their contents and compositions are essential for evaluation of medicinal potency. Therefore, we optimized the simultaneous quantitative determination of the hydrolysable tannins using ultra-performance liquid chromatography. A column of ethylene bridged hybrid (BEH) phenyl (C6 alkyl phenyl group as solid-phase modification) was shown to be most effective for the separation of hydrolysable tannins isolated from R. rugosa and related compounds when the column temperature was kept under 25°C. The efficacy of the BEH phenyl column might be due to the interaction between solid phase and phenolic ester groups of hydrolysable tannins such as galloyl, hexahydroxydiphenoyl and valoneoyl groups. The relation between the retention times on the BEH phenyl column and the column temperature was demonstrated to depend on the structural characteristics of hydrolysable tannins.


Hydrolysable tannins UPLC Quantitative determination Rosa rugosa 


  1. 1.
    Gault SM, Synge PM (1971) The dictionary of roses in colour. Ebury, LondonGoogle Scholar
  2. 2.
    Iwatani S (1857) Kochi-Yojyo-Ko. Owned by Akita Prefectural Library, A498–14Google Scholar
  3. 3.
    Dictionary of Chinese Medicine, vol 4 (1985) Shanghai Science-Technology Publication, Shogakukan, Tokyo, pp 2440–2441Google Scholar
  4. 4.
    Hashidoko Y (1996) The phytochemistry of Rosa rugosa. Phytochemistry 43(3):535–549CrossRefGoogle Scholar
  5. 5.
    Lee HJ, Ahn JW, Lee BJ, Moon SG, Seo Y (2004) Antioxidant activity of Rosa rugosa. Korean J Biotechnol Bioeng 19(1):67–71Google Scholar
  6. 6.
    Kamijo M, Kanazawa T, Funaki M, Nishizawa M, Yamagishi T (2008) Effects of Rosa rugosa petals on intestinal bacteria. Biosci Biotechnol Biochem 72(3):773–777CrossRefPubMedGoogle Scholar
  7. 7.
    Toda M, Kawabata J, Kasai T (2001) Inhibitory effects of Ellagi- and Gallotannins on Rat intestinal α-glucosidase complexes. Biosci Biotechnol Biochem 65(3):542–547CrossRefPubMedGoogle Scholar
  8. 8.
    Wilkins CK, Bohm BA (1976) Ellagitannins from Tellima grandiflora. Phytochemistry 15:211–214CrossRefGoogle Scholar
  9. 9.
    Hatano T, Ogawa N, Yasuhara T, Okuda T (1990) Tannins of rosaceous plants. VIII. Hydrolyzable tannin monomers having a valoneoyl group from flower petals of Rosa rugosa Thunb. Chem Pharm Bull 38:3308–3313Google Scholar
  10. 10.
    Hatano T, Ogawa N, Shingu T, Okuda T (1990) Tannins of rosaceous plants. IX. Rugosins D, E, F and G, dimeric and trimeric hydrolyzable tannins with valoneoyl group(s), from flower petals of Rosa rugosa Thunb. Chem Pharm Bull 38:3341–3346Google Scholar
  11. 11.
    Okuda T, Yoshida T, Ashida M, Yazaki K (1983) Tannins of casuarina and stachyurus species. Part 1. Structures of pendunculagin, casuarictin, strictinin, casuarinin, casuariin, and stachyurin. J Chem Soc Perkin Trans 1:1765–1772CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2010

Authors and Affiliations

  • Sarangowa Ochir
    • 1
  • ByoungJae Park
    • 1
  • Makoto Nishizawa
    • 2
  • Tsutomu Kanazawa
    • 3
  • Minoru Funaki
    • 1
  • Takashi Yamagishi
    • 1
    Email author
  1. 1.Kitami Institute of TechnologyKitamiJapan
  2. 2.Faculty of Bio-IndustryTokyo University of AgricultureAbashiriJapan
  3. 3.Harunire Bio Laboratory Co., Ltd.KitamiJapan

Personalised recommendations