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Journal of Natural Medicines

, Volume 64, Issue 3, pp 383–387 | Cite as

Simultaneous determination of hydrolysable tannins in the petals of Rosa rugosa and allied plants

  • Sarangowa Ochir
  • ByoungJae Park
  • Makoto Nishizawa
  • Tsutomu Kanazawa
  • Minoru Funaki
  • Takashi YamagishiEmail author
Note

Abstract

The petals of Rosa rugosa and allied plants for medicinal use contain abundant hydrolysable tannins, and they show remarkable biological activities. The activities are dependent on the structures of the hydrolysable tannins, so their contents and compositions are essential for evaluation of medicinal potency. Therefore, we optimized the simultaneous quantitative determination of the hydrolysable tannins using ultra-performance liquid chromatography. A column of ethylene bridged hybrid (BEH) phenyl (C6 alkyl phenyl group as solid-phase modification) was shown to be most effective for the separation of hydrolysable tannins isolated from R. rugosa and related compounds when the column temperature was kept under 25°C. The efficacy of the BEH phenyl column might be due to the interaction between solid phase and phenolic ester groups of hydrolysable tannins such as galloyl, hexahydroxydiphenoyl and valoneoyl groups. The relation between the retention times on the BEH phenyl column and the column temperature was demonstrated to depend on the structural characteristics of hydrolysable tannins.

Keywords

Hydrolysable tannins UPLC Quantitative determination Rosa rugosa 

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2010

Authors and Affiliations

  • Sarangowa Ochir
    • 1
  • ByoungJae Park
    • 1
  • Makoto Nishizawa
    • 2
  • Tsutomu Kanazawa
    • 3
  • Minoru Funaki
    • 1
  • Takashi Yamagishi
    • 1
    Email author
  1. 1.Kitami Institute of TechnologyKitamiJapan
  2. 2.Faculty of Bio-IndustryTokyo University of AgricultureAbashiriJapan
  3. 3.Harunire Bio Laboratory Co., Ltd.KitamiJapan

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