Inhibitory effect of the flowers of artichoke (Cynara cardunculus) on TPA-induced inflammation and tumor promotion in two-stage carcinogenesis in mouse skin
The methanol extract of the flowers of artichoke (Cynara cardunculus) exhibited remarkable antitumor activity in an in vivo two-stage carcinogenesis test in mice, using 7,12-dimethylbenz[a]anthracene as an initiator and 12-O-tetradecanoylphorbol-13-acetate (TPA) as a promoter. From the active fraction of the methanol extract, four triterpene alcohols and their corresponding acetates were isolated and identified. These compounds were evaluated for their inhibitory effects on TPA-induced inflammation (1 μg/ear) in mice and showed marked anti-inflammatory effects, with a 50% inhibitory dose of 0.50–0.91 μmol/ear.
KeywordsCancer chemoprevention Two-stage carcinogenesis Artichoke Cynaracardunculus
This work was supported, in a part, by “High-Tech Research Center” Project for Private Universities: matching fund subsidy from MEXT (Ministry of Education, Culture, Sports, Science and Technology), 2007–2009, of Japan. We would also like to thank Dr. Yumiko Kimura and Dr. Kouichi Metori (Analytical Center, College of Pharmacy, Nihon University) for NMR and mass spectroscopy measurements.
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