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Journal of Natural Medicines

, Volume 63, Issue 4, pp 463–467 | Cite as

Effect of Gastrodia elata on tumor necrosis factor-alpha-induced matrix metalloproteinase activity in endothelial cells

  • Yun Jung Lee
  • Sun Mi Hwang
  • Dae Gill Kang
  • Jin Sook Kim
  • Ho Sub LeeEmail author
Note

Abstract

The aim of the present study was to investigate whether an ethanol extract of Gastrodia elata (EGE) rhizome, a traditional Korean herbal medical food, suppresses the endothelial extracellular matrix degradation induced by tumor necrosis factor (TNF)-α. Gelatin zymography results showed that pretreatment with EGE to human umbilical vein endothelial cells (HUVEC) decreased TNF-α-induced increase of matrix metalloproteinase (MMP)-2/-9 activities in the range of 1–50 μg/ml. Real-time qRT-PCR results also revealed that TNF-α-induced MMP-2/-9 mRNA expression levels were attenuated by pretreatment with EGE. These results provide new insights into the pathophysiological mechanisms for the anti-atherosclerotic properties of EGE in vascular diseases.

Keywords

Gastrodia elata Matrix metalloproteinase Atherosclerosis Endothelial cell Vascular disease 

Notes

Acknowledgments

This work was supported by a research grant from the Korea Science and Engineering Foundation (KOSEF) funded by the Korean Government (MEST) (no. R13-2008-028-01000-0).

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2009

Authors and Affiliations

  • Yun Jung Lee
    • 1
    • 2
  • Sun Mi Hwang
    • 1
  • Dae Gill Kang
    • 1
  • Jin Sook Kim
    • 3
  • Ho Sub Lee
    • 1
    • 2
    Email author
  1. 1.Professional Graduate School of Oriental MedicineWonkwang UniversityIksanRepublic of Korea
  2. 2.Medical Resources Research Institute (MeRRI)Wonkwang UniversityIksanRepublic of Korea
  3. 3.Korea Institute of Oriental MedicineDaejeonRepublic of Korea

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