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Appraisal of immunomodulatory potential of Spirulina fusiformis: an in vivo and in vitro study

  • Mahaboobkhan RasoolEmail author
  • Evan Prince Sabina
Original Paper

Abstract

In recent years, Spirulina has gained more and more attention from medical scientists as a nutraceutical and a source of potential pharmaceuticals. The present study was conducted to elucidate the immunomodulatory effect of Spirulina fusiformis (a cyanobacterium of the family Oscillatoriaceae) in vivo and in vitro. The in vivo effect of S. fusiformis (400 or 800 mg/kg body wt.) on humoral immune response, cell-mediated immune response and tumour necrosis factor alpha was investigated in mice. We also evaluated the effect of S. fusiformis (50 or 100 μg/ml) in vitro on mitogen (phytohaemagglutinin)-induced T lymphocyte proliferation in heparinized human peripheral blood. For comparison, dexamethasone was used as a standard. In mice, S. fusiformis (400 or 800 mg/kg body wt.) administration significantly inhibited the humoral immune response, cell-mediated immune response (delayed-type hypersensitivity reaction (DTH)) and tumour necrosis factor alpha in a dose-dependent manner. In vitro, S. fusiformis (50 or 100 μg/ml) decreased the mitogen (phytohaemagglutinin)-induced T lymphocyte proliferation in a concentration-dependent manner when compared with control cells. These observations clearly suggest that S. fusiformis has a remarkable immunosuppressive effect, which provides a scientific validation for the popular use of this drug, and helped us in further work on investigating its complete mechanism of action.

Keywords

Spirulina fusiformis Dexamethasone Lymphocyte proliferation Humoral immune response Cell-mediated immune response Tumour necrosis factor alpha 

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2008

Authors and Affiliations

  1. 1.Faculty of Heath and Life SciencesManagement and Science UniversityShah AlamMalaysia
  2. 2.School of Biotechnology, Chemical and Biomedical EngineeringVIT UniversityVelloreIndia

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