Antidiarrhoeal activity of the standardised extract of Cinnamomum tamala in experimental rats
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The present study was designed to investigate the antidiarrhoeal potential of 50% ethanolic extract of Cinnamomum tamala on experimentally induced castor oil diarrhoea, gastric emptying of phenol red meal, gastrointestinal transit of charcoal meal and in vitro mast cell degranulation activity. C. tamala extract (25, 50 and 100 mg/kg, orally) produced a dose-dependent reduction in the total amount of faecal matter in castor oil-induced diarrhoea. The mean distance travelled by charcoal meal at 50 and 100 mg/kg of extract showed a significant reduction in the secretion of gastrointestinal fluid accumulation by 32.5–65.0%. The Na+ and K+ concentrations on castor oil-induced fluid accumulation showed a greater inhibitory effect on Na+ levels than on K+ concentrations. C. tamala significantly reduced the lipid peroxidation (P < 0.001) and increased the catalase (P < 0.01) activity in comparison to the castor oil-induced groups. C. tamala leaf extract did not show any significant effect at a higher dose (15 mg/ml) on mast cell degranulation. However, the extract in the dose of 5 and 10 mg/ml conferred significant mast cell protective action (P < 0.001). The percentage of eugenol in extract is 3.8% w/w, and total tannin is 247.5 mg/g. The result indicates the Indian spice C. tamala is useful for diarrhoea.
KeywordsCinnamomum tamala Antidiarrhoeal Indian spice
This study was supported by grants from the Department of Science and Technology, Ministry of Science and Technology, New Delhi. Authors are highly thankful to Dr. M. P. Dubey, Retired Scientist and Head, Department of Pharmacology, Central Drug Research Institute, Lucknow, India, for his critical advice during the course of study. Authors are also thankful to the Director of NBRI for providing necessary facilities.
- 2.Sharma SR, Dwivedi SK, Swarup D (1996) Hypoglycaemic and hypolipidemic effects of Cinnamomum tamala Nees leaves. Indian J Exp Biol 34:372–374Google Scholar
- 4.Melo L, Thomas G, Mukherjee R (1988) Antidiarrhoeal activity of bisnordihydrotoxiferine isolated from root bark of Strychonus trinervis (Vell.) Mart. J Pharm Pharmacol 40:79–82Google Scholar
- 6.Norton S (1954) Quantitative determination of mast cell fragmentation of compound 48/80. Br J Pharmacol 9:494–497Google Scholar
- 13.Claiborne A (1985) Catalase activity. In: Greenwald RA (ed) CRS handbook of methods for oxygen radical research. CRC Press, Boca Raton, pp 283–284Google Scholar
- 15.Kakkar P, Das B, Viswanathan PN (1984) Modified spectrophotometric assay of SOD. Ind J Biochem Biophys 2:130–132Google Scholar
- 20.Bruton LL (1985) Agent affecting gastrointestinal water flux and motility, digestants and bile acids. In: Gillman AG, Rall TW, Nies AS, Tayloy P (eds) The pharmacological basis of therapeutics. McGraw-Hill, New York, p 914Google Scholar