Advertisement

Journal of Natural Medicines

, Volume 62, Issue 2, pp 244–246 | Cite as

Potential anthelmintic: d-psicose inhibits motility, growth and reproductive maturity of L1 larvae of Caenorhabditis elegans

  • Masashi Sato
  • Hiroyuki Kurose
  • Toru YamasakiEmail author
  • Ken Izumori
Note

Abstract

No anthelmintic sugars have yet been identified. Eight ketohexose stereoisomers (d- and l-forms of psicose, fructose, tagatose and sorbose), along with d-galactose and d-glucose, were examined for potency against L1 stage Caenorhabditis elegans fed Escherichia coli. Of the sugars, d-psicose specifically inhibited the motility, growth and reproductive maturity of the L1 stage. d-Psicose probably interferes with the nematode nutrition. The present results suggest that d-psicose, one of the rare sugars, is a potential anthelmintic.

Keywords

d-Psicose Caenorhabditis elegans Inhibitory effect Motility Developmemt Anthelmintic 

Notes

Acknowledgments

The authors wish to thank Prof. Kazuhiro Fukada of Kagawa University for valuable discussions on this topic. This research was supported in part by a grant from the Glyco-Biocluster Plan Based on Rare Sugars of the Ministry of Education, Science, Sports and Culture of Japan.

References

  1. 1.
    Mukai D, Matsuda N, Yoshioka Y, Sato M, Yamasaki T (2008) Potential anthelmintics: polyphenols of tea plant Camellia sinensis L. produce lethal toxicity in Caenorhabditis elegans. J Nat Med 62 (in press)Google Scholar
  2. 2.
    Kiuchi F, Tsuda Y, Yoshimura H, Nishioka I, Nonaka G (1998) Studies on crude drugs effective on visceral larva migrans. III. The bursting activity of tannins on dog roundworm larva. Chem Pharm Bull 36:1796–1802Google Scholar
  3. 3.
    Yamasaki T, Sato M, Mori T, Mohamed ASA, Fujii K, Tsukioka J (2002) Toxicity of tannins towards the free-living nematode Caenorhabditis elegans and the brine shrimp Artemia salina. J Nat Toxins 11:165–171PubMedGoogle Scholar
  4. 4.
    Goto I, Inaba M, Shimizu T, Maede Y (1994) Mechanism of hemolysis of canine erythrocytes induced by l-sorbose. Am J Vet Res 55:291–294PubMedGoogle Scholar
  5. 5.
    Masson S, Henriksen O, Stengaard A, Thomsen C, Quistorff B (1994) Hepatic metabolism during constant infusion of fructose; comparative studies with 31P-magnetic resonance spectroscopy in man and rats. Biochim Biophys Acta 1199:166–174PubMedGoogle Scholar
  6. 6.
    Buemann B, Gesmar H, Astrup A, Quistorff B (2000) Effects of oral d-tagatose, a stereoisomer of d-fructose, on liver metabolism in man as examined by 31P-magnetic resonance spectroscopy. Metabolism 49:1335–1339PubMedCrossRefGoogle Scholar
  7. 7.
    Lewis JA, Fleming JT (1995) Basic culture methods. In: Epstein HF, Shakes DC (eds) Methods in cell biology 48. Caenorhabditis elegans: modern biological analysis of an organism. Academic Press, New York, pp 3–29Google Scholar
  8. 8.
    Hough L, Stacey BE (1963) The occurrence of d-ribohexulose in Itea ilicifolia, Itea virginica, and Itea yunnanensis. Phytochemistry 2:315–320CrossRefGoogle Scholar
  9. 9.
    Schroeder W, Hoeksema H (1959) A new antibiotic, 6-amino-9-d-psicofuranosylpurine. J Am Chem Soc 81:1767–1768CrossRefGoogle Scholar
  10. 10.
    Takeshita K, Suga A, Takada G, Izumori K (2000) Mass production of d-psicose from d-fructose by a continuous bioreactor system using immobilized d-tagatose 3-epimerase. J Biosci Bioeng 90:453–455PubMedGoogle Scholar
  11. 11.
    Itoh H, Izumori K (1996) Enzymatic production of l-tagatose and l-fructose from l-sorbose and l-psicose, respectively. J Ferment Bioeng 81:351–353CrossRefGoogle Scholar
  12. 12.
    Muniruzzaman S, Tokunaga H, Izumori K (1995) Conversion of d-psicose to allitol by Enterobacter agglomerans strain 221e. J Ferment Bioeng 79:323–327CrossRefGoogle Scholar
  13. 13.
    Takeshita K, Shimonishi T, Izumori K (1996) Production of l-psicose from allitol by Gluconobacter frateurii IFO 3254. J Ferment Bioeng 81:212–215CrossRefGoogle Scholar
  14. 14.
    Muniruzzaman S, Tokunaga H, Izumori K (1994) Isolation of Enterobacter agglomerans strain 221e from soil, a potent d-tagatose producer from galactitol. J Ferment Bioeng 78:145–148CrossRefGoogle Scholar
  15. 15.
    Itoh H, Sato T, Takeuchi T, Khan AR, Izumori K (1995) Preparation of d-sorbose from d-tagatose by immobilized d-tagatose 3-epimerase. J Ferment Bioeng 79:184–185CrossRefGoogle Scholar
  16. 16.
    Kiuchi F, Miyashita N, Tsuda Y, Kondo K, Yoshimura H (1987) Studies on crude drugs effective on visceral larva migrans. I. Identification of larvicidal principles in betel nuts. Chem Pharm Bull 35:2880–2886PubMedGoogle Scholar
  17. 17.
    Fujiwara M, Sengpta P, McIntire SL (2002) Regulation of body size and behavioral state of C. elegans by sensory perception and the EGL-4 cGMP-dependent protein kinase. Neuron 36:1091–1102PubMedCrossRefGoogle Scholar

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2008

Authors and Affiliations

  • Masashi Sato
    • 1
  • Hiroyuki Kurose
    • 1
  • Toru Yamasaki
    • 1
    Email author
  • Ken Izumori
    • 2
  1. 1.Department of Applied Biological ScienceKagawa UniversityKagawaJapan
  2. 2.Rare Sugar Research CenterKagawa UniversityKagawaJapan

Personalised recommendations