Efficacy of extracting solvents to chemical components of kava (Piper methysticum) roots

  • Tran Dang Xuan
  • Masakazu Fukuta
  • Ao Chang Wei
  • Abdelnaser Abdelghany Elzaawely
  • Tran Dang Khanh
  • Shinkichi Tawata
Note

Abstract

The chemical composition of kava (Piper methysticum) lactones and various phytochemicals obtained following the sonication of ground kava roots extracted in the solvents hexane, chloroform, acetone, ethanol, methanol and water, respectively, was analyzed. Eighteen kava lactones, cinnamic acid bornyl ester and 5,7-dimethoxy-flavanone, known to be present in kava roots, were identified, and seven compounds, including 2,5,8-trimethyl-1-naphthol, 5-methyl-1-phenylhexen-3-yn-5-ol, 8,11-octadecadienoic acid-methyl ester, 5,7-(OH)2-4′-one-6,8-dimethylflavanone, pinostrobin chalcone and 7-dimethoxyflavanone-5-hydroxy-4′, were identified for the first time. Glutathione (26.3 mg/g) was found in the water extract. Dihydro-5,6-dehydrokavain (DDK) was present at a higher level than methysticin and desmethoxyyagonin, indicating that DDK is also a major constituent of kava roots. Acetone was the most effective solvent in terms of maximum yield and types of kava lactones isolated, followed by water and chloroform, whereas hexane, methanol, and ethanol were less effective as solvents. Total phenolic and antioxidant activity varied among the extracting solvents, with acetone and chloroform producing the highest effects, followed by water, while methanol, ethanol and hexane were less effective.

Keywords

Antioxidant Extracting solvent Kava lactone Kava root Piper methysticum Total phenolics 

Notes

Acknowledgments

The authors express sincere thanks to the Grand-in-Aid for Scientific Research (No. 18880020) by Japan Society for the Promotion of Science (JSPS) to Dr. Tran Dang Xuan.

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2007

Authors and Affiliations

  • Tran Dang Xuan
    • 1
  • Masakazu Fukuta
    • 1
  • Ao Chang Wei
    • 1
  • Abdelnaser Abdelghany Elzaawely
    • 1
  • Tran Dang Khanh
    • 2
  • Shinkichi Tawata
    • 1
  1. 1.Department of Bioscience and Biotechnology, Faculty of AgricultureUniversity of the RyukyusOkinawaJapan
  2. 2.Faculty of Crop ScienceKonkuk UniversitySeoulRepublic of Korea

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