Efficacy of extracting solvents to chemical components of kava (Piper methysticum) roots
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The chemical composition of kava (Piper methysticum) lactones and various phytochemicals obtained following the sonication of ground kava roots extracted in the solvents hexane, chloroform, acetone, ethanol, methanol and water, respectively, was analyzed. Eighteen kava lactones, cinnamic acid bornyl ester and 5,7-dimethoxy-flavanone, known to be present in kava roots, were identified, and seven compounds, including 2,5,8-trimethyl-1-naphthol, 5-methyl-1-phenylhexen-3-yn-5-ol, 8,11-octadecadienoic acid-methyl ester, 5,7-(OH)2-4′-one-6,8-dimethylflavanone, pinostrobin chalcone and 7-dimethoxyflavanone-5-hydroxy-4′, were identified for the first time. Glutathione (26.3 mg/g) was found in the water extract. Dihydro-5,6-dehydrokavain (DDK) was present at a higher level than methysticin and desmethoxyyagonin, indicating that DDK is also a major constituent of kava roots. Acetone was the most effective solvent in terms of maximum yield and types of kava lactones isolated, followed by water and chloroform, whereas hexane, methanol, and ethanol were less effective as solvents. Total phenolic and antioxidant activity varied among the extracting solvents, with acetone and chloroform producing the highest effects, followed by water, while methanol, ethanol and hexane were less effective.
KeywordsAntioxidant Extracting solvent Kava lactone Kava root Piper methysticum Total phenolics
The authors express sincere thanks to the Grand-in-Aid for Scientific Research (No. 18880020) by Japan Society for the Promotion of Science (JSPS) to Dr. Tran Dang Xuan.
- 3.Singh YN, Blumenthal M (1997) Kava, an overview. HerbalGram 39:34–55Google Scholar
- 4.Chevallier A (1996) The encyclopedia of medicinal plants. DK Publ, New YorkGoogle Scholar
- 5.Leung AY, Foster S (1996) Encyclopedia of common natural ingredients. Wiley, New YorkGoogle Scholar
- 6.Mors WB, Magalhaes MT, Gottlieb OR (1962) Naturally occurring aromatic derivatives of monocyclic α-pyrones. In: Par R (ed) Progress in the chemistry of organic natural products, vol 20. Vienna New York Springer, pp 131–161Google Scholar
- 7.Sahelian R (1998) Clinical uses of kava. In kava, the miracle antianxiety herb. St Martin’s Press, New York, pp 85–88Google Scholar
- 10.Stickel F, Baumuller HM, Seitz K, Vasilakis D, Seitz G, Seitz HK, Schuppan D (2002) Hepatitis induced by kava (Piper methysticum rhizoma). J Hetatol 39:62–67Google Scholar
- 11.Zou L, Henderson GL, Harkey MR, Sakai Y, Li A (2004) Effects of kava (kava–kava, ‘Awa', Yaqona, Piper methysticum) on c-DNA-expressed cytochrome P450 enzymes and human cryopreserved hepatocytes. Phytomedicine 11:285–294Google Scholar
- 23.Bilia AR, Scalise L, Bergonzi MC, Vincieri FF (2004) Analysis of kavalactones from Piper methysticum (kava-kava). J Chromatogr B 812:203–214Google Scholar