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Journal of Natural Medicines

, Volume 62, Issue 1, pp 117–121 | Cite as

Authentication of Stephania tetrandra S. Moore (Fang Ji) and differentiation of its common adulterants using microscopy and HPLC analysis

  • Vaishali C. JoshiEmail author
  • Bharathi Avula
  • Ikhlas A. Khan
Note

Abstract

Stephania tetrandra S. Moore (Hang Fang Ji) is used in traditional Chinese medicine as a diuretic, an antiphlogistic, and an antirheumatic. The name “fang ji” is applied to at least four different genera of plants, including Aristolochia fangchi Y. C. Wu ex L. D. Chow and S. M. Hwang, Cocculus orbiculatus (L.) DC., Stephania tetrandra S. Moore, and Sinomenium acutum Rehder and E. H. Wilson. Due to similarity in the use of their common names, Stephania tetrandra S. Moore is often confused with Aristolochia fangchi Y. C. Wu ex L. D. Chow and S. M. Hwang, which has potentially dangerous consequences. To aid rapid and easy differentiation between the roots of these four species, so as to avoid possible contamination, detailed macroscopic and microscopic observations were made using stereo-and light-microscopy. The powdered samples were further analyzed using HPLC.

Keywords

Authentication Stephania tetrandra Aristolochia fangchi Cocculus orbiculatus Sinomenium acutum Microscopy and HPLC 

Notes

Acknowledgments

This work was funded in part by the Food and Drug Administration contract “Botanical Dietary Supplements: Science-Base for Authentication”, FD-U-002071.01. We are also thankful to Beijing Yuke Botanical Development Co. Ltd. China and Division of Chinese Materia Medica for providing authenticated samples and to Roy Upton for his comments and suggestions.

References

  1. 1.
    De Smet P, Keller K, Hansel R (1992) In: Chandler R (ed) Adverse effects of herbal drugs, vol 1. Springer, New YorkGoogle Scholar
  2. 2.
    Chen HA, Xu GJ, Jin R, Xu LS (1990) Hong Kong samples of the traditional Chinese medicine “Fang Ji” contains aristolochic acid toxins. China J Chin Mat Med 15:707–708Google Scholar
  3. 3.
    Zhu M, Phillipson JD (1996) Hong Kong samples of the traditional Chinese medicine “fang ji” contain aristolochic acid toxins. Int J Pharmacognosy 34:283–289CrossRefGoogle Scholar
  4. 4.
    Huang KC (1999) The pharmacology of Chinese Herbs. CRC, Boca RatonGoogle Scholar
  5. 5.
    Venhaerweghem JL, Depierreux M, Tielemans C, Abramowicz D, Dratwa M, Jadoul M, Richard C, Vandervelde D, Verbeelen D, Vanhaelen-Faster R (1993) Rapidly progressive interstitial renal fibrosis in young women: association with slimming regimen including Chinese herbs. Lancet 341:387–391CrossRefGoogle Scholar
  6. 6.
    Nortier JL, Deschodt-Lanckman MM, Simon S, Thielemans NO, de Prez EG, Depierreux MF, Tielemans CL, Richard C, Lauwerys RR, Bernard AM, Vanherweghem JL (1997) Proximal tubular injury in Chinese herbs nephropathy: monitoring by neutral endopeptidase enzymuria. Kidney Int 51:288–293PubMedCrossRefGoogle Scholar
  7. 7.
    Antonia JC, Geoffrey M, Terry C, Geoffrey CK, Simmonds MSJ, Pusey CD (2002) Aristolochic acid as a causative factor in a case of Chinese herbal nephropathy. Nephrol Dial Transpl 17:524–525CrossRefGoogle Scholar
  8. 8.
    Blatter A, Reich E (2004) Qualitative and quantitative HPTLC method for quality control of Stephania tetrandra. J Liquid Chromatogr Relat Technol 27:2087–2100CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2007

Authors and Affiliations

  • Vaishali C. Joshi
    • 1
    Email author
  • Bharathi Avula
    • 1
  • Ikhlas A. Khan
    • 1
    • 2
  1. 1.National Center for Natural Products Research, Research Institute of Pharmaceutical Science, School of PharmacyUniversity of MississippiUniversityUSA
  2. 2.Department of Pharmacognosy, School of PharmacyUniversity of MississippiUniversityUSA

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