Isolation and identification of antioxidants from Pedilanthus tithymaloides
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A bioassay-guided methodology utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH), the trolox equivalent antioxidant capacity (TEAC), and reducing power assays, as well as an assessment of scavenging properties against O 2 ·− , H2O2, HOCl, ROO·, ·NO, and ONOO− were used to find the main antioxidant principles of Pedilanthus tithymaloides (Euphorbiaceae), a shrub used in traditional Cuban medicine. The principles were identified as kaempferol 3-O-β-D-glucopyranoside-6′′-(3-hydroxy-3-methylglutarate), quercitrin, isoquercitrin, and scopoletin. The contents of total phenolics and flavonoids were found to be 76.0 ± 4.8 mg of gallic acid equivalents/g extract and 9.8 ± 0.4 mg of rutin equivalents/g extract, respectively.
KeywordsPedilanthus tithymaloides Flavonoid Coumarin Antioxidant activity Anti-inflammatory activity
REQUIMTE for financial support, Fundação para a Ciência e Tecnologia and Fundo Social Europeu for post-doc (Matthew S) and PhD (Costa D) grants, and Programme Alβan for scholarship E0D033475CU.
- 1.Abreu P, Matthew S, González T, Costa D, Segundo MA, Fernandes E (2006) Anti-inflammatory and antioxidant activity of a medicinal tincture from Pedilanthus tithymaloides. Life Sci 78:1578–1585, and references cited thereinGoogle Scholar
- 3.Harborne JB (1994) The flavonoids. Chapman & Hall, LondonGoogle Scholar
- 17.Comalada M, Camuesco D, Sierra S, Ballester I, Xaus J, Gálvez J, Zarzuelo (2005) A in vivo quercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-кB pathway. Eur J Immunol 35:584–592Google Scholar
- 19.Kim JS, Kim JC, Shim SH, Jin EJL, Bae K, Son KH, Kim HP, Kang SS, Chang HW (2006) Chemical constituents of the root of Dystaenia takeshimana and their anti-inflammatory activity. Arch Pharm Res 8:617–623Google Scholar