Journal of Natural Medicines

, Volume 62, Issue 1, pp 63–66 | Cite as

The relationship between 1-deoxynojirimycin content and α-glucosidase inhibitory activity in leaves of 276 mulberry cultivars (Morus spp.) in Kyoto, Japan

  • Kazuhisa Yatsunami
  • Masatoshi Ichida
  • Satoshi Onodera


The relationship between 1-deoxynojirimycin (DNJ) content and α-glucosidase inhibitory activity in mulberry (Morus) leaves is discussed. Mulberry leaves were collected from the Center for Bioresource Field Science, Kyoto Institute of Technology, Kyoto, Japan on 19 May, 9 July, and 9 August, 2003. Mulberry leaves were extracted with 75% ethanol. The inhibitory activity for rat intestinal crude enzyme was measured using maltose. The content of DNJ in the extracts was measured using HPLC. The mean DNJ content in the 0.04–0.06% range was high in collected samples. The inhibitory activities in July and August were higher (P < 0.01) than in May, and the activity in July was higher (P < 0.01) than in August. A strong correlation (r = 0.901, r2 = 0.811, n = 15) existed between DNJ content and α-glucosidase inhibition in leaves of Morus bombycis harvested in July. Similarly, correlation coefficients of the other mulberry varieties in July were higher than they were in May or August. The inhibitory activity and the DNJ content of Morus latifolia in August were lower than for any other mulberry variety. These results show that the high inhibitory cultivars harvested in July, except for M. latifolia, are more suited to products that contain high DNJ contents.


Mulberry Morus spp. 1-Deoxynojirimycin α-Glucosidase Inhibitor Relationship 



This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (KAKENHI: 18510206).


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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2007

Authors and Affiliations

  • Kazuhisa Yatsunami
    • 1
  • Masatoshi Ichida
    • 2
  • Satoshi Onodera
    • 3
  1. 1.Faculty of AgricultureTamagawa UniversityTokyoJapan
  2. 2.Center for Bioresource Field ScienceKyoto Institute of TechnologyKyotoJapan
  3. 3.Department of Clinical and Biomedical SciencesShowa Pharmaceutical UniversityTokyoJapan

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