Journal of Natural Medicines

, Volume 61, Issue 4, pp 443–448 | Cite as

Antinociceptive, anti-inflammatory and antipyretic effects of Muntingia calabura aqueous extract in animal models

  • Z. A. ZakariaEmail author
  • N. A. Mohd Nor Hazalin
  • S. N. H. Mohd Zaid
  • M. Abdul Ghani
  • M. H. Hassan
  • H. K. Gopalan
  • M. R. Sulaiman


The present study was carried out to elucidate the potential of aqueous extract of Muntingia calabura leaves aqueous extract (MCAE) as antinociceptive, anti-inflammatory and antipyretic agents using the formalin-, carrageenan-induced paw edema- and brewer’s yeast-induced pyrexia tests in rats. The extract was prepared by soaking the dried powdered leaves of M. calabura in distilled water (dH2O) overnight. The supernatant obtained, considered as a stock solution (100% concentration/strength), was then diluted to concentrations of 10% and 50% and used together in all experimental models. The MCAE, at concentrations of 10%, 50% and 100%, were found to show significant antinociceptive, anti-inflammatory and antipyretic activities in all tests. However, all of the activities occurred in a concentration-independent manner. The 50% and 100% concentrations of MCAE produced insignificant antinociceptive and antipyretic activities, respectively. Although the 100% concentration of MCAE produced significant (P<0.05) anti-inflammatory activity, the activity was lower than that of the 10% and 50% concentrations of MCAE. Based on the results, we conclude that the M. calabura leaves possessed antinociceptive, anti-inflammatory and antipyretic activities and, thus, justifies the Peruvian folklore claims of its medicinal values.


Muntingia calabura Aqueous extract Antinociceptive Anti-inflammatory Antipyretic 



This study was supported by a research grant of the Universiti Industri Selangor, Malaysia (project code number: 03013; project vote number: 3090103013). The authors would like to thank the Universiti Putra Malaysia for the use of their facilities.


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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2007

Authors and Affiliations

  • Z. A. Zakaria
    • 1
    Email author
  • N. A. Mohd Nor Hazalin
    • 2
  • S. N. H. Mohd Zaid
    • 2
  • M. Abdul Ghani
    • 2
  • M. H. Hassan
    • 2
  • H. K. Gopalan
    • 2
  • M. R. Sulaiman
    • 3
  1. 1.Faculty of PharmacyUniversiti Teknologi MARAShah AlamMalaysia
  2. 2.Faculty of Biotechnology and Life SciencesUniversiti Industri SelangorShah AlamMalaysia
  3. 3.Department of Biomedical Sciences, Faculty of Medicine and Health ScienceUniversiti Putra MalaysiaUPM SerdangMalaysia

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