Journal of Natural Medicines

, Volume 61, Issue 2, pp 131–137

Anticancer properties of panduratin A isolated from Boesenbergia pandurata (Zingiberaceae)

  • Chandra Kirana
  • Graham Peter Jones
  • Ian Roland Record
  • Graeme Howie McIntosh
Original Paper


Extract of Boesenbergia pandurata (Kaempferia pandurata) (Zingiberaceae) has been used as a replacement for K. rotunda, the main ingredient of a popular traditional tonic called “jamu” especially for women in Indonesia. From our previous study, ethanolic extract of B. pandurata showed strong inhibitory effects on the growth of cancer cells, similar to ethanolic extract of Curcuma longa. C. longa and its bioactive compound, curcumin, have shown potential anticancer activity in in vitro and in vivo studies and have undergone clinical trials. Panduratin A, a chalcone derivative isolated from B. pandurata, was found to inhibit the growth of MCF-7 human breast cancer and HT-29 human colon adenocarcinoma cells with an IC50 of 3.75 and 6.56 µg/ml, respectively. Panduratin A arrested cancer cells labelled with Annexin-V and propidium iodide in the G0/G1 phase and induced apoptosis in a dose-dependent manner. In an animal model study, male Sprague–Dawley rats were fed with AIN diet containing ethanolic extracts prepared from the equivalent of 4% by weight of dried rhizomes of B. pandurata and C. longa. Aberrant crypt foci (ACFs) were induced by two subcutaneous doses (15 mg/kg body weight) of azoxymethane (AOM) 1 week apart. The rats were killed 10 weeks later, and the ACFs were assessed in the colon. At the dose given to rats, it appeared that the extracts were not toxic. Total ACFs were slightly reduced by B. pandurata extract compared to control group but not significantly different. Extract of B. pandurata may have a protective effect against colon cancer but additional studies using different models, such as a breast cancer model, need to be carried out.


Panduratin A Boesenbergia pandurata Zingiberaceae Apoptosis Cell cycle arrest Azoxymethane Aberrant crypt foci 


  1. 1.
    Wills RBH, Bone K, Morgan M (2000) Herbal products: active constituents, modes of action and quality control. Nutr Res Rev 13:47–77CrossRefPubMedGoogle Scholar
  2. 2.
    Hyene K (1987) Zingiberaceae. In: Tumbuhan Berguna Indonesia Book 1 (in Indonesian). Badan Litban Kehutanan, Jakarta, pp 567–605Google Scholar
  3. 3.
    Kirana C, Record IR, McIntosh GH, Jones GP (2003) Screening for antitumor activity of 11 species of Indonesian Zingiberaceae using human MCF-7 and HT-29 cancer cells. Pharm Biol 41:271–276CrossRefGoogle Scholar
  4. 4.
    Jaipetch T, Kanghae S, Pancharoen O, Patrick VA, Reutrakul V, Tuntiwachwuttikul P, White AH (1982) Constituents of Boesenbergia pandurata (syn Kaempferia pandurata): isolation, crystal structure and synthesis of (±)-boesenbergin A. Aust J Chem 25:351–361CrossRefGoogle Scholar
  5. 5.
    Mahidol C, Tunticachwuttikul P, Reutrakul V, Taylor WC (1984) Constituents of Boesenbergia pandurata (syn. Kaempferia pandurata). III Isolation and synthesis of (±)-boesenbergin B. Aust J Chem 37:1739–1745Google Scholar
  6. 6.
    Pandji C, Grimm C, Wray V, Witte L, Proksch P (1993) Insecticidal constituents from four species of the Zingiberaceae. Phytochemistry 34:415–419CrossRefGoogle Scholar
  7. 7.
    Surh Y-J (1999) Molecular mechanisms of chemopreventive effect of selected dietary and medicinal phenolic substances. Mutat Res 428:305–327PubMedGoogle Scholar
  8. 8.
    Murakami A, Jiwajinda S, Koshimizu K, Ohigashi H (1995) Screening for in vitro tumor promoting activities of edible plants from Thailand. Cancer Lett 95:139–146PubMedCrossRefGoogle Scholar
  9. 9.
    Trakoontivakorn G, Nakahara K, Shinmoto H, Takenaka M, Onishi-Kameyama M, Ono H, Yoshida M, Nagata T, Tsushida T (2001) Structural analysis of a novel antimutagenic compound 4-hydroxypanduratin A, and the antimutageic activity of flavonoids in a Thai spice, fingerroot (Boesenbergia pandurata Shult.), against mutagenic heterocyclic amines. J Agric Food Chem 49:3046–3050PubMedGoogle Scholar
  10. 10.
    Tuchinda P, Reutrakul V, Claeson P, Pongprayoon U, Sematong T, Santisuk T, Taylor WC (2002) Antiinflammatory cyclohexenyl chalcone derivatives in Boesenbergia pandurata. Phytochemistry 50:160–173Google Scholar
  11. 11.
    Yun JM, Kwon H, Mukhtar H, Hwang JK (2005) Induction of apoptosis by panduratin A isolated from Kaempferia pandurata in human colon caner HT-29 cells. Planta Med 71:501–507PubMedCrossRefGoogle Scholar
  12. 12.
    Pretlow TP, Barrow BJ, Ashton WS, O’Rriordan MA, Pretlow TG, et al (1991) Aberrant crypts: putative preneoplastic foci in human colonic mucosa. Cancer Res 51:1564–1567PubMedGoogle Scholar
  13. 13.
    Bird RP, Good CK (2000) The significance of aberrant crypt foci in understanding the pathogenesis of colon cancer. Toxicol Lett 112–113:395–402PubMedCrossRefGoogle Scholar
  14. 14.
    Corpet DE, Tache S (2002) Most effective colon cancer chemopreventive agents in rats: a systematic review of aberrant crypt foci and tumor data, ranked by potency. Nutr Cancer 43:1–21PubMedCrossRefGoogle Scholar
  15. 15.
    Tuntiwachwuttikul P, Pancharoen O, Reutrakul V, Byrne LT (1984) (1′RS, 2′SR, 6′RS)-(2-6-dihydroxy-4-methoxyphenyl)[3′-methyl-2′-(3″-methylbut-2″-enyl)-6′-phenylcyclohex-3′-enyl]methanone (panduratin-A)-a constituent of the red rhizomes of a variety of Boesenbergia pandurata. Aust J Chem 37:449–453CrossRefGoogle Scholar
  16. 16.
    He XG, Lin LZ, Lian LZ, Lindenmaier M (1998) Liquid chromatography electrospray mass spectrometric analysis of curcuminoids and sesquiterpenoids in turmeric (Curcuma longa). J Chromatogr A 818:127–132CrossRefGoogle Scholar
  17. 17.
    Hansen MB, Nielsen SE, Berg K (1989) Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 119:203–210PubMedCrossRefGoogle Scholar
  18. 18.
    Reeves PG, Neilsen FH, Fahey GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951PubMedGoogle Scholar
  19. 19.
    Holy JM (2002) Curcumin disrupts mitotic spindle structure and induces micronucleation in MCF-7 breast cancer cells. Mutat Res 518:71–84PubMedGoogle Scholar
  20. 20.
    Azuine MA, Bhide SV (1992) Chemopreventive effect of turmeric against stomach and skin tumor induced by chemical carcinogens in Swiss mice. Nutr Cancer 17:77–83PubMedCrossRefGoogle Scholar
  21. 21.
    Fahey JW, Stephenson K (2002) Pinostrobin from honey and Thai ginger (Boesenbergia pandurata): a potent flavonoid inducer of mammalian phase-2 chemoprotective and antioxidant enzymes. J Agric Food Chem 50:7472–7476PubMedCrossRefGoogle Scholar
  22. 22.
    Schultz M, Dutta S, Tew KD (1997) Inhibitors of glutathione S-transferases as therapeutic agents. Adv Drug Deliver Rev 26:91–104CrossRefGoogle Scholar
  23. 23.
    Reddy B, Rao C, Seibert K (1996) Evaluation of cyclooxygenase-2 inhibitor for potential chemopreventive properties in colon carcinogenesis. Cancer Res 56:4566–4569PubMedGoogle Scholar

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2006

Authors and Affiliations

  • Chandra Kirana
    • 1
    • 2
  • Graham Peter Jones
    • 3
  • Ian Roland Record
    • 1
  • Graeme Howie McIntosh
    • 1
  1. 1.CSIRO Human NutritionAdelaide BCAustralia
  2. 2.Department of BiologyFaculty of Mathematics and Natural Sciences, Brawijaya UniversityMalangIndonesia
  3. 3.Faculty of Science, School of Agriculture, Food and WineAdelaide UniversityAdelaideAustralia

Personalised recommendations