Zusammenfassung
Zum Nachweis der Helicobacter-pylori-Infektion stehen eine große Anzahl von Methoden zur Verfügung. Glücklicherweise ist die Qual bei der Auswahl geringer als vermutet: die unterschiedlichen Testverfahren kommen differenziert zum Einsatz. Grundsätzlich werden invasive von nichtinvasiven Nachweismethoden unterschieden. Bei den invasiven Methoden stellen Kultur, Histologie und Urease-Schnelltest die Standardverfahren da. Bei den nichtinvasiven Verfahren sind der 13C-Harnstoff-Atemtest und der Stuhltest die Methoden der Wahl. Einige Neuerungen, Weiterentwicklungen und Innovation hat es in letzter Zeit gegeben. Auch diese sollen hier kritisch beleuchtet werden.
Abstract
Many different methods are available for detection of Helicobacter pylori infection. The selection of the method of choice is not that difficult because every test has a field of application. In principle invasive and non-invasive methods are differentiated. Culture, histology, and rapid urease test are the standard invasive detection methods. Methods of choice for non-invasive detection of H. pylori are the 13C urea breath test and the stool antigen test. Several improvements, developments and innovations have been described recently and are critically discussed in this review.
Literatur
Best LM, Haldane DJ, Keelan M et al. (2003) Multilaboratory comparison of proficiencies in susceptibility testing of Helicobacter pylori and correlation between agar dilution and E test methods. Antimicrob Agents Chemother 47: 3138–3144
Chey WD (2000) Accurate diagnosis of helicobacter pylori. 14C-urea breath test. Gastroenterol Clin North Am 29: 895–902
el Zimaity HM, Graham DY, al Assi MT et al. (1996) Interobserver variation in the histopathological assessment of Helicobacter pylori gastritis. Hum Pathol 27: 35–41
Figueiredo C, Machado JC, Pharoah P et al. (2002) Helicobacter pylori and interleukin 1 genotyping: an opportunity to identify high-risk individuals for gastric carcinoma. J Natl Cancer Inst 94: 1680–1687
Gatta L, Vakil N, Ricci C et al. (2004) Effect of proton pump inhibitors and antacid therapy on 13C urea breath tests and stool test for Helicobacter pylori infection. Am J Gastroenterol 99: 823–829
Gisbert JP, Abraira V (2006) Accuracy of Helicobacter pylori diagnostic tests in patients with bleeding peptic ulcer: a systematic review and meta-analysis. Am J Gastroenterol 101: 848–863
Gisbert JP, de la Morena F, Abraira V (2006) Accuracy of monoclonal stool antigen Test for the diagnosis of H. pylori infection: a systematic review and meta-analysis. Am J Gastroenterol 101: 1921–1930
Gisbert JP, Pajares JM (2004) Review article: C-urea breath test in the diagnosis of Helicobacter pylori infection – a critical review. Aliment Pharmacol Ther 20: 1001–1017
Glocker E, Kist M (2004) Rapid detection of point mutations in the gyrA gene of Helicobacter pylori conferring resistance to ciprofloxacin by a fluorescence resonance energy transfer-based real-time PCR approach. J Clin Microbiol 42: 2241–2246
Glocker E, Berning M, Gerrits MM et al. (2005) Real-time PCR screening for 16S rRNA mutations associated with resistance to tetracycline in Helicobacter pylori. Antimicrob Agents Chemother 49: 3166–3170
Kiesslich R, Goetz M, Burg J et al. (2005) Diagnosing Helicobacter pylori in vivo by confocal laser endoscopy. Gastroenterology 128: 2119–2123
Labenz J, Barsch G, Peitz U et al. (1996) Validity of a novel biopsy urease test (HUT) and a simplified 13C-urea breath test for diagnosis of Helicobacter pylori infection and estimation of the severity of gastritis. Digestion 57: 391–397
Leodolter A, Dominguez-Munoz JE, Von Arnim U et al. (1999) Validity of a modified 13C-urea breath test for pre- and posttreatment diagnosis of Helicobacter pylori infection in the routine clinical setting. Am J Gastroenterol 94: 2100–2104
Leodolter A, Dominguez-Munoz JE, Von Arnim U et al. (1999) Citric acid or orange juice for the 13C-urea breath test: the impact of pH and gastric emptying. Aliment Pharmacol Ther 13: 1057–1062
Leodolter A, Vaira D, Bazzoli F et al. (2003) European multicentre validation trial of two new non-invasive tests for the detection of Helicobacter pylori antibodies: urine-based ELISA and rapid urine test. Aliment Pharmacol Ther 18: 927–931
Leodolter A, Wolle K, Peitz U et al. (2003) Helicobacter pylori genotypes and expression of gastritis in erosive gastro-oesophageal reflux disease. Scand J Gastroenterol 38: 498–502
Loy CT, Irwig LM, Katelaris PH, Talley NJ (1996) Do commercial serological kits for Helicobacter pylori infection differ in accuracy? A meta-analysis. Am J Gastroenterol 91: 1138–1144
Malfertheiner P, Holtmann G, Peitz U et al. (2001) [Guidelines of the German Society of Digestive and Metabolic Diseases for treatment of dyspepsia]. Z Gastroenterol 39: 937–956
Malfertheiner P, Megraud F, O’Morain C et al. (2002) Current concepts in the management of Helicobacter pylori infection – the Maastricht 2–2000 consensus report. Aliment Pharmacol Ther 16: 167–180
Oleastro M, Menard A, Santos A et al. F (2003) Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori. J Clin Microbiol 41: 397–402
Pantoflickova D, Scott DR, Sachs G et al. (2003) 13C urea breath test (UBT) in the diagnosis of Helicobacter pylori: why does it work better with acid test meals? Gut 52: 933–937
Peitz U, Hackelsberger A, Malfertheiner P (1999) A practical approach to patients with refractory Helicobacter pylori infection, or who are re-infected after standard therapy. Drugs 57: 905–920
Schabereiter-Gurtner C, Hirschl AM, Dragosics B et al. (2004) Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens. J Clin Microbiol 42: 4512–4518
Scholte GH, van Doorn LJ, Cats A et al. (2002) Genotyping of Helicobacter pylori in paraffin-embedded gastric biopsy specimens: relation to histological parameters and effects on therapy. Am J Gastroenterol 97: 1687–1695
Tseng CA, Wang WM, Wu DC (2005) Comparison of the clinical feasibility of three rapid urease tests in the diagnosis of Helicobacter pylori infection. Dig Dis Sci 50: 449–452
Uemura N, Okamoto S, Yamamoto S et al. (2001) Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 345: 784–789
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Leodolter, A., Labenz, J. Diagnostik der Helicobacter-pylori-Infektion. Gastroenterologe 1, 253–258 (2006). https://doi.org/10.1007/s11377-006-0043-8
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DOI: https://doi.org/10.1007/s11377-006-0043-8