Journal of Biomedical Science

, 15:771 | Cite as

Enhancement of ectopic bone formation by bone morphogenetic protein-2 delivery using heparin-conjugated PLGA nanoparticles with transplantation of bone marrow-derived mesenchymal stem cells

  • Sung Eun Kim
  • Oju Jeon
  • Jung Bok Lee
  • Min Soo Bae
  • Heoung-Jae Chun
  • Seong-Hwan Moon
  • Il Keun Kwon
Original Paper


This study was performed to determine if a combination of previously undifferentiated bone marrow-derived mesenchymal stem cells (BMMSCs) and exogenous bone morphogenetic protein-2 (BMP-2) delivered via heparin-conjugated PLGA nanoparticles (HCPNs) would extensively regenerate bone in vivo. In vitro testing found that the HCPNs were able to release BMP-2 over a 2-week period. Human BMMSCs cultured in medium containing BMP-2-loaded HCPNs for 2 weeks differentiated toward osteogenic cells expressing alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN) mRNA, while cells without BMP-2 expressed only ALP. In vivo testing found that undifferentiated BMMSCs with BMP-2-loaded HCPNs induce far more extensive bone formation than either implantation of BMP-2-loaded HCPNs or osteogenically differentiated BMMSCs. This study demonstrates the feasibility of extensive in vivo bone regeneration by transplantation of undifferentiated BMMSCs and BMP-2 delivery via HCPNs.


Bone marrow-derived mesenchymal stem cell Bone morphogenetic protein-2 Bone regeneration Heparin-conjugated PLGA nanoparticles 


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Copyright information

© National Science Council Taipei 2008

Authors and Affiliations

  • Sung Eun Kim
    • 1
  • Oju Jeon
    • 2
  • Jung Bok Lee
    • 1
  • Min Soo Bae
    • 1
  • Heoung-Jae Chun
    • 3
  • Seong-Hwan Moon
    • 4
  • Il Keun Kwon
    • 1
  1. 1.Department of Oral Biology and Institute of Oral Biology, School of DentistryKyung Hee UniversitySeoulSouth Korea
  2. 2.Weldon School of Biomedical Engineering and Department of PharmaceuticsPurdue UniversityWest LafayetteUSA
  3. 3.Department of Mechanical Engineering, School of EngineeringYonsei UniversitySeoulSouth Korea
  4. 4.Department of Orthopaedic Surgery, School of MedicineYonsei UniversitySeoulSouth Korea

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