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Glycine input induces the synaptic facilitation in salamander rod photoreceptors

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Abstract

Glycinergic synapses in photoreceptors are made by centrifugal feedback neurons in the network, but the function of the synapses is largely unknown. Here we report that glycinergic input enhances photoreceptor synapses in amphibian retinas. Using specific antibodies against a glycine transporter (GlyT2) and glycine receptor β subunit, we identified the morphology of glycinergic input in photoreceptor terminals. Electrophysiological recordings indicated that 10 μM glycine depolarized rods and activated voltage-gated Ca2+ channels in the neurons. The effects facilitated glutamate vesicle release in photoreceptors, meanwhile increased the spontaneous excitatory postsynaptic currents in Off-bipolar cells. Endogenous glycine feedback also enhanced glutamate transmission in photoreceptors. Additionally, inhibition of a Cl uptake transporter NKCC1 with bumetanid effectively eliminated glycine-evoked a weak depolarization in rods, suggesting that NKCC1 maintains a high Cl level in rods, which causes to depolarize in responding to glycine input. This study reveals a new function of glycine in retinal synaptic transmission.

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Acknowledgement

Grants: This work was supported by the National Eye Institute grant (EY14161, WS).

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Correspondence to Wen Shen.

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Shen, W., Jiang, Z. & Li, B. Glycine input induces the synaptic facilitation in salamander rod photoreceptors. J Biomed Sci 15, 743 (2008). https://doi.org/10.1007/s11373-008-9263-x

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Keywords

  • Interplexiform cells
  • Cl transporters
  • Glycinergic synapses
  • Retinas