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Journal of Biomedical Science

, Volume 12, Issue 6, pp 949–959 | Cite as

Chrysoeriol potently inhibits the induction of nitric oxide synthase by blocking AP-1 activation

  • Doo-Youn Choi
  • Jeong Yong Lee
  • Mi-Ran Kim
  • Eun-Rhan Woo
  • Yoon Gyoon Kim
  • Keon Wook Kang
Article

Summary

Chrysoeriol is a flavonoid with antioxidant and anti-inflammatory activities. Despite the large number of studies performed on its biological activities, no clear picture of its mode of action has emerged. In the present study, we isolated chrysoeriol from the leaves of Digitalis purpurea (foxglove), and studied its effect on the induction of the inducible nitric oxide synthase (iNOS) gene, and the mechanism of this induction in Raw264.7 macrophages. Chrysoeriol pretreatment potently inhibited the release of NO in the cells treated with lipopolysaccharide (LPS), and Western blot and RT-PCR analyses revealed that chrysoeriol inhibited the LPS-induced inductions of iNOS gene. Moreover, it is known that the activations of nuclear factor-κB (NF-κB) and activator protein 1 (AP-1) are crucial steps in the transcriptional activation of the iNOS gene. Here, we found that chrysoeriol selectively suppressed AP-1 activation, and that activation of AP-1 is likely to be essential for iNOS induction in LPS-treated macrophages. This presumed inhibitory effect on AP-1 activation by chrysoeriol may be associated with its potent NO blocking and anti-inflammatory effects.

Keywords:

Anti-inflammatory activity AP-1 Chrysoeriol iNOS Nitric oxide 

Abbreviations

AP-1

activator protein-1

DMEM

Dulbecco’s modified Eagle’s medium

ERK

Extracellular signal-regulated kinase

FCS

fetal calf serum

iNOS

inducible nitric oxide synthase

JNK

c-Jun N-terminal kinase

LPS

lipopolysaccharide

NF-κB

nuclear Factor-κB

NO

nitric oxide

S16r

S16 ribosomal protein

SDS

sodium dodecyl sulfate

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Notes

Acknowledgements

This work was supported in part by a grant from the Regional University Research Program (E-00216; Kang K.W.) funded by Korea Research Foundation and the Plant Diversity Research Center 21st Century Frontier Research Program funded by the Korean Ministry of Science and Technology (Grant No. PF0320505-00; Woo E.).

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Copyright information

© National Science Council Taipei 2005

Authors and Affiliations

  • Doo-Youn Choi
    • 1
  • Jeong Yong Lee
    • 2
  • Mi-Ran Kim
    • 1
  • Eun-Rhan Woo
    • 1
    • 2
  • Yoon Gyoon Kim
    • 3
  • Keon Wook Kang
    • 2
  1. 1.Research Center for Proteineous MaterialsChosun UniversityGwangjuSouth Korea
  2. 2.College of PharmacyChosun UniversityGwangjuSouth Korea
  3. 3.Department of Pharmacology, College of MedicineDangook UniversityCheonanSouth Korea

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