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The better suppression of pepper Phytophthora blight by arbuscular mycorrhizal (AM) fungus than Purpureocillium lilacinum alone or combined with AM fungus

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Abstract

Purpose

Phytophthora blight caused by Phytophthora capsici (Pc) is one of the most economically destructive soilborne diseases of pepper (Capsicum annum L.) on a global scale. Biocontrol using antagonistic microbes, such as Purpureocillium lilacinum (Pl) and arbuscular mycorrhizal (AM) fungus Funneliformis caledonium (Fc), is one of the significant strategies for ecologically sound plant disease management. The purpose of this work was to investigate the sole and combined suppression of pepper Phytophthora blight by Fc and Pl.

Materials and methods

The 14-week pot experiment with three pepper plants per pot included five treatments: control (non-inoculation), inoculation with Pc, inoculation with Pc and Pl (Pc + Pl), inoculation with Pc and Fc (Pc + Fc), and inoculation with Pc, Pl, and Fc (Pc + Pl + Fc). Pots were randomly arranged with eight replicates per treatment. The incidence and severity of Phytophthora blight at plant full productive stage were recorded. The biomasses and N, P, and K concentrations of pepper shoots, roots, and fruits were all measured. In addition, root mycorrhizal colonization rate and soil pH, phosphatase activity, and available P concentration were also tested.

Results and discussion

The inoculation of Pc induced both high incidence (92%) and severity (33%) of pepper Phytophthora blight, and the alleviating effects of Pl, Fc, and Pl + Fc were 46%, 79%, and 59%, respectively. The Fc significantly increased (P < 0.05) root mycorrhizal colonization, nutrient (N, P, and K) acquisition, plant biomass, and fruit yield of pepper, while Pl only significantly increased (P < 0.05) plant nutrient (N and P) acquisition and tended to increase the fruit yield. The Pc, Pl, and Fc all had additive effects on decreasing soil pH, but only Fc significantly increased (P < 0.05) soil phosphatase activity and available P concentration, contributing partly to the elevated P acquisition as well as the increased P concentrations in both shoot and root.

Conclusions

Compared with the sole inoculation of Fc, the extra inoculation of Pl had negative effects on mycorrhizal colonization, soil P mobilization, and plant growth and nutrient acquisition. It suggests that AM fungus (Fc) has the superiority of formatting symbioses with plant roots and enhancing soil P mobilization for the suppression of pepper Phytophthora blight compared with Pl, and the joint application of different fungal agents to improve plant health needs careful consideration.

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Acknowledgments

We would like to acknowledge Ms. Yu Zhang and Ms. Hongmin Liu for their assistance in the pot experiment and three anonymous reviewers for their suggestions on manuscript revision.

Funding

This work was supported by the National Key R&D Program (2017YFD0200603) of China, the National Natural Science Foundation (No.41671265) of China, the Knowledge Innovation Program (ISSASIP1634) of Chinese Academy of Sciences (CAS), and the Talents Project (Y412010009) of State Key Laboratory of Soil and Sustainable Agriculture, China. Junli Hu is supported by the fellowship of the Youth Innovation Promotion Association (No. 2016285), CAS.

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Correspondence to Xiangui Lin.

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Hu, J., Hou, S., Li, M. et al. The better suppression of pepper Phytophthora blight by arbuscular mycorrhizal (AM) fungus than Purpureocillium lilacinum alone or combined with AM fungus. J Soils Sediments 20, 792–800 (2020). https://doi.org/10.1007/s11368-019-02438-9

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Keywords

  • Funneliformis caledonium
  • Nutrient acquisition
  • Phytophthora capsici
  • Soil phosphatase
  • Soil available P
  • Soil pH