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Journal of Soils and Sediments

, Volume 20, Issue 1, pp 584–593 | Cite as

Microbiological analysis of cadmium-contaminated sediments during biostabilization with indigenous sulfate-reducing bacteria

  • Weihua Peng
  • Xiaomin Li
  • Manli Lin
  • Wenhong FanEmail author
Sediments, Sec 5 • Sediment Management • Research Article

Abstract

Purpose

Sulfate-reducing bacteria (SRB) have received particular attention in the bioremediation of sediments contaminated with heavy metals. In this study, indigenous SRB were used to stabilize Cd in sediments spiked with different Cd concentrations (≤ 600 mg kg−1).

Materials and methods

The study investigated the Cd leaching efficiency from sediments after 166 days (d) of biotreatment and assessed the bacterial community and bacteria relationship in sediments during SRB biostabilization.

Results and discussion

The study found that the Cd leaching efficiency of sediments was reduced by 18.1–40.3% (29.4 ± 8.7%) after 166 days of biotreatment. During the biostabilization, the bacterial community in sediments significantly changed, particularly after 61 days of biotreatment. At the family level, the identified dominant bacteria (mean abundance > 3%) included Bacillaceae, norank Nitrospira, Anaerolineaceae, Nitrospinaceae, Streptococcaceae, and Hydrogenophilaceae. The study also speculated the complex relationships between these bacteria. The relative abundance of Desulfobacteraceae and Desulfobulbaceae in sediments was enhanced after biotreatment. Bacillaceae and Streptococcaceae may play a negative role in Cd biostabilization and inhibited SRB biological activity. However, Anaerolineaceae and Hydrogenophilaceae may have commensalism and mutualism relationships, respectively, with typical SRB. The presence of Nitrospinacea and norank Nitrospira may reduce the inhibitive effect of denitrifying bacteria on SRB, thereby exhibiting a positive effect on biologic sulfate reduction and Cd biostabilization.

Conclusions

Indigenous SRB treatment increased Cd stability in sediments and changed bacterial community. During SRB biostabilization, complex relationships between bacteria in sediments were speculated, including competition, syntrophism, and antagonism. These results provide insights for better regulating and controlling SRB biostabilization.

Keywords

Biostabilization Cadmium Heavy metals Microbiological analysis Sediment Sulfate-reducing bacteria 

Notes

Funding information

This work was supported by the National Natural Science Foundation of China (51778031, 21707006, and 51708012), University Natural Science Research Project of Anhui Province (KJ2017A445), Excellent Youth Talent Support Program of Suzhou University (SZXYQNL2017002), Natural Science Projects of Colleges and Universities in Anhui Province (KJ2018A0450), and Natural Science Foundation of Anhui Province (1708085QE125).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Resources and Civil EngineeringSuzhou UniversitySuzhouPeople’s Republic of China
  2. 2.School of Space and EnvironmentBeihang UniversityBeijingPeople’s Republic of China
  3. 3.National Engineering Research Center of Coal Mine Water Hazard ControllingSuzhou UniversitySuzhouPeople’s Republic of China
  4. 4.Key Laboratory of Mine Water Resource Utilization of Anhui Higher Education InstituteSuzhou UniversitySuzhouPeople’s Republic of China
  5. 5.Beijing Advanced Innovation Center for Big Data-Based Precision MedicineBeihang UniversityBeijingPeople’s Republic of China

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