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Effect of organic matter manipulation on the seasonal variations in microbial composition and enzyme activities in a subtropical forest of China

  • Xiaohua Wan
  • Xiang Li
  • Changpeng Sang
  • Zhihong Xu
  • Zhiqun HuangEmail author
Soils, Sec 1 • Soil Organic Matter Dynamics and Nutrient Cycling • Research Article
  • 24 Downloads

Abstract

Purpose

The aim of this study was to determine the impacts of reduced aboveground and belowground C inputs on the community composition of soil microbes and enzyme activities in a seasonal context.

Materials and methods

Litterfall removal, root exclusion, and stem girdling treatments under a subtropical conifer plantation growing on a coarse texture of sandy soil in southeast China were employed. One year after the initiation of the treatments, we measured the soil microbial biomass, community composition, and enzyme activities, including hydrolytic and oxidative extracellular enzymes on a seasonal basis. Soil inorganic N, dissolved organic C and N, and available P were also determined.

Results and discussion

Seasonal variations of soil microbial composition and enzyme activities were attributed to soil temperature and moisture and soil nutrient availability. Girdling treatments significantly increased the abundances of gram-negative bacteria and actinomycetes in winter when soil temperature, moisture, and available nutrients were at the lowest level among the four seasons. Girdling alone and girdling combined with litter removal and root trenching significantly decreased the cellobiohydrolase, β-glucosidase, β-1,4-N-acetylglucosaminidase, and acid phosphatase activities in autumn. These hydrolytic enzyme activities were significantly correlated with soil moisture, NH4+, DOC, and available P. We also found a significant relationship between hydrolytic enzyme activities and the ratio of gram-positive to gram-negative bacteria.

Conclusions

Plant belowground C allocation, soil temperature, and moisture drove the seasonal patterns of soil microbial composition and enzyme activities. Labile C input by root exudates is a key determinant of ecosystem functions mediated by soil microbes such as microbial decomposition processes.

Keywords

Enzyme activity Girdling Litterfall removal Root trenching Soil microbial composition 

Notes

Funding information

The research was supported by the National Science Fund for Distinguished Young Scholars (31625007), the National Natural Science Foundation of China (41371269 and 31600495), Fujian Natural Science Foundation (2018J01714), and the project funded by Education Department of Fujian Province (JAT160113).

Supplementary material

11368_2019_2300_MOESM1_ESM.docx (49 kb)
ESM 1 (DOCX 49 kb).

References

  1. Baldrian P (2009) Ectomycorrhizal fungi and their enzymes in soils: is there enough evidence for their role as facultative soil saprotrophs? Oecologia 161(4):657–660CrossRefGoogle Scholar
  2. Bardgett RD, Bowman WD, Kaufmann R, Schmidt SK (2005) A temporal approach to linking aboveground and belowground ecology. Trends Ecol Evol 20(11):634–641CrossRefGoogle Scholar
  3. Barea J, Pozo MJ, Azco’n R, Azco’n-Aguilar C (2005) Microbial co-operation in the rhizosphere. J Exp Bot 56(417):1761–1778CrossRefGoogle Scholar
  4. Binkley D, Stape JL, Takahashi EN, Ryan MG (2006) Treegirdling to separate root and heterotrophic respiration in two Eucalyptus stands in Brazil. Oecologia 148:447–454CrossRefGoogle Scholar
  5. Brant JB, Myrold DD, Sulzman EW (2006) Root controls on soil microbial community structure in forest soils. Oecologia 148(4):650–659CrossRefGoogle Scholar
  6. Brockett BFT, Prescott CE, Grayston SJ (2012) Soil moisture is the major factor influencing microbial community structure and enzyme activities across seven biogeoclimatic zones in western Canada. Soil Biol Biochem 44(1):9–20CrossRefGoogle Scholar
  7. Chen DM, Zhou LX, Wu JP, Hsu J, Lin YBFSL (2012) Tree girdling affects the soil microbial community by modifying resource availability in two subtropical plantations. Appl Soil Ecol 53(2):108–115CrossRefGoogle Scholar
  8. Conn C, Dighton J (2000) Litter quality influences on decomposition, ectomycorrhizal community structure and mycorrhizal root surface acid phosphatase activity. Soil Biol Biochem 32(4):489–496CrossRefGoogle Scholar
  9. Drenovsky R, Vo D, Graham K, Scow K (2004) Soil water content and organic carbon availability are major determinants of soil microbial community composition. Microb Ecol 48(3):424–430CrossRefGoogle Scholar
  10. Fekete I, Varga C, Kotroczó Z, Tóth JA, Várbiró G (2011) The relation between various detritus inputs and soil enzyme activities in a Central European deciduous forest. Geoderma 167-168(8):15–21CrossRefGoogle Scholar
  11. Frostegård Å, Tunlid A, Bååth E (2011) Use and misuse of PLFA measurements in soils. Soil Biol Biochem 43(8):1621–1625CrossRefGoogle Scholar
  12. Högberg P, Nordgren A, Buchmann N, Taylor AF, Ekblad A, Högberg MN, Nyberg G, Ottosson-Lofvenius M, Read DJ (2001) Large-scale forest girdling shows that current photosynthesis drives soil respiration. Nature 411:789–792CrossRefGoogle Scholar
  13. Högberg M, Högberg P, Myrold D (2007) Is microbial community composition in boreal forest soils determined by pH, C-to-N ratio, the trees, or all three? Oecologia 150(4):590–601CrossRefGoogle Scholar
  14. Jones DL, Hodge A, Kuzyakov Y (2004) Plant and mycorrhizal regulation of rhizodeposition. New Phytol 163(3):459–480CrossRefGoogle Scholar
  15. Kaiser C, Koranda M, Kitzler B, Fuchslueger L, Schnecker J, Schweiger P, Rasche F, Zechmeister-Boltenstern S, Sessitsch A, Richter A (2010) Belowground carbon allocation by trees drives seasonal patterns of extracellular enzyme activities by altering microbial community composition in a beech forest soil. New Phytol 187(3):843–858CrossRefGoogle Scholar
  16. Karolien D, Dries R, Mihiricwmanimel W, Peter L, Pascal B (2009) Microbial community composition and rhizodeposit-carbon assimilation in differently managed temperate grassland soils. Soil Biol Biochem 41(1):144–153CrossRefGoogle Scholar
  17. Koranda M, Kaiser C, Fuchslueger L, Kitzler B, Sessitsch A, Zechmeisterboltenstern S, Richter A (2013) Seasonal variation in functional properties of microbial communities in beech forest soil. Soil Biol Biochem 60(100):95–104CrossRefGoogle Scholar
  18. Kotroczó Z, Veres Z, Fekete I, Krakomperger Z, Tóth JA, Lajtha K, Tóthmérész B (2014) Soil enzyme activity in response to long-term organic matter manipulation. Soil Biol Biochem 70(2):237–243CrossRefGoogle Scholar
  19. Kramer C, Gleixner G (2008) Soil organic matter in soil depth profiles: distinct carbon preferences of microbial groups during carbon transformation. Soil Biol Biochem 40(2):425–433CrossRefGoogle Scholar
  20. Kramer C, Trumbore S, Fröberg M, Dozal LMC, Zhang DC, Xu XM, Santos GM, Hanson PJ (2010) Recent (4 year old) leaf litter is not a major source of microbial carbon in a temperate forest mineral soil. Soil Biol Biochem 42(7):1028–1037CrossRefGoogle Scholar
  21. Kuzyakov Y, Cheng W (2001) Photosynthesis controls of rhizosphere respiration and organic matter decomposition. Soil Biol Biochem 33(14):1915–1925CrossRefGoogle Scholar
  22. Laclau JP, Levillain J, Deleporte P, Nzila JDD, Bouillet JP, André LS, Versini A, Mareschal L, Nouvellon Y, M’Bou AT (2010) Organic residue mass at planting is an excellent predictor of tree growth in Eucalyptus plantations established on a sandy tropical soil. For Ecol Manag 260(12):2148–2159CrossRefGoogle Scholar
  23. Landesman WJ, Dighton J (2010) Response of soil microbial communities and the production of plant-available nitrogen to a two-year rainfall manipulation in the New Jersey Pinelands. Soil Biol Biochem 42(10):1751–1758CrossRefGoogle Scholar
  24. Li YJ, Yang XD, Zou XM, Wu JH (2009) Response of soil nematode communities to tree girdling in a subtropical evergreen broad-leaved forest of southwest China. Soil Biol Biochem 41(5):877–882CrossRefGoogle Scholar
  25. López-Mondéjar R, Zühlke D, Becher D, Riedel K, Baldrian P (2016) Cellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems. Sci Rep 6:25279CrossRefGoogle Scholar
  26. Markp W, Donaldr Z (2006) Response of oxidative enzyme activities to nitrogen deposition affects soil concentrations of dissolved organic carbon. Ecosystems 9(6):921–933CrossRefGoogle Scholar
  27. Mccarthy AJ, Williams ST (1992) Actinomycetes as agents of biodegradation in the environment--a review. Gene 115(1–2):189–192CrossRefGoogle Scholar
  28. Nemergut DR, Cleveland CC, Wieder WR, Washenberger CL, Townsend AR (2010) Plot-scale manipulations of organic matter inputs to soils correlate with shifts in microbial community composition in a lowland tropical rain forest. Soil Biol Biochem 42(12):2153–2160CrossRefGoogle Scholar
  29. Pisani O, Lin LH, Lun OOY, Lajtha K, Nadelhoffer KJ, Simpson AJ, Simpson MJ (2016) Long-term doubling of litter inputs accelerates soil organic matter degradation and reduces soil carbon stocks. Biogeochem 127(1):1–14CrossRefGoogle Scholar
  30. Rustad LE, Huntington TG, Boone RD (2000) Controls on soil respiration: implications for climate change. Biogeochem 48(1):1–6CrossRefGoogle Scholar
  31. Saiya-Cork KR, Sinsabaugh RL, Zak DR (2002) The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol Biochem 34(9):1309–1315CrossRefGoogle Scholar
  32. Sardans J, Peñuelas J (2005) Drought decreases soil enzyme activity in a Mediterranean Quercus ilex L. forest. Soil Biol Biochem 37(3):455–461Google Scholar
  33. Schimel J, Balser TC, Wallenstein M (2007) Microbial stress-response physiology and its implications for ecosystem function. Ecology 88(6):1386–1394CrossRefGoogle Scholar
  34. Schmidt MWI, Torn MS, Abiven S, Dittmar T, Guggenberger G, Janssens IA, Kleber M, Kögelknabner I, Lehmann J, Manning DAC (2011) Persistence of soil organic matter as an ecosystem property. Nature 478(7367):49–56CrossRefGoogle Scholar
  35. Sinsabaugh R, Lauber C, Weintraub M, Ahmed B, Allison S, Crenshaw C, Contosta A, Cusack D, Frey S, Gallo M (2008) Stoichiometry of soil enzyme activity at global scale. Ecol Lett 11(11):1252–1264CrossRefGoogle Scholar
  36. Stevenson BA, Hunter DWF, Rhodes PL (2014) Temporal and seasonal change in microbial community structure of an undisturbed, disturbed, and carbon-amended pasture soil. Soil Biol Biochem 75:175–185CrossRefGoogle Scholar
  37. Štursová M, Žifčáková L, Leigh MB, Burgess R, Baldrian P (2012) Cellulose utilization in forest litter and soil: identification of bacterial and fungal decomposers. FEMS Microbiol Ecol 80:735–746CrossRefGoogle Scholar
  38. Swallow M, Quideau SA, Mackenzie MD, Kishchuk BE (2009) Microbial community structure and function: the effect of silvicultural burning and topographic variability in northern Alberta. Soil Biol Biochem 41(4):770–777CrossRefGoogle Scholar
  39. Ushio M, Balser TC, Kitayama K (2013) Effects of condensed tannins in conifer leaves on the composition and activity of the soil microbial community in a tropical montane forest. Plant Soil 365(1–2):157–170CrossRefGoogle Scholar
  40. Veres Z, Kotroczó Z, Fekete I, Tóth JA, Lajtha K, Townsend K, Tóthmérész B (2015) Soil extracellular enzyme activities are sensitive indicators of detrital inputs and carbon availability. Appl Soil Ecol 92:18–23CrossRefGoogle Scholar
  41. Versini A, Laclau JP (2014) The role of harvest residues to sustain tree growth and soil nitrogen stocks in a tropical Eucalyptus plantation. Plant Soil 376(1–2):245–260CrossRefGoogle Scholar
  42. Wang QK, He TX, Wang SL, Li L (2013) Carbon input manipulation affects soil respiration and microbial community composition in a subtropical coniferous forest. Agric For Meteorol 178-179(17):152–160CrossRefGoogle Scholar
  43. Wardle D, Bardgett R, Klironomos J, Setälä H, van der PW, Wall D (2004) Ecological linkages between aboveground and belowground biota. Science 304(5677):1629–1633CrossRefGoogle Scholar
  44. White DC, Davis WM, Nickels JS, King JD, Bobbie RJ (1979) Determination of the sedimentary microbial biomass by extractible lipid phosphate. Oecologia 40(1):51–62CrossRefGoogle Scholar
  45. Wu JP, Liu ZF, Wang XL, Sun YX, Zhou LX, Lin YB, Fu SL (2011) Effects of understory removal and tree girdling on soil microbial community composition and litter decomposition in two Eucalyptus plantations in South China. Funct Ecol 25(4):921–931CrossRefGoogle Scholar
  46. Yarwood SA, Myrold DD, Högberg MN (2009) Termination of belowground C allocation by trees alters soil fungal and bacterial communities in a boreal forest. FEMS Microbiol Ecol 70(1):151–162CrossRefGoogle Scholar
  47. Zhao Q, Classen AT, Wang WW, Zhao XR, Mao B, Zeng DH (2016) Asymmetric effects of litter removal and litter addition on the structure and function of soil microbial communities in a managed pine forest. Plant Soil 414(1–2):1–13Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Xiaohua Wan
    • 1
    • 2
  • Xiang Li
    • 3
  • Changpeng Sang
    • 1
    • 2
  • Zhihong Xu
    • 4
  • Zhiqun Huang
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Subtropical Mountain Ecology (Funded by Ministry of Science and Technology and Fujian Province)Fujian Normal UniversityFuzhouChina
  2. 2.College of Geographical ScienceFujian Normal UniversityFuzhouChina
  3. 3.Maintenance Branch Company of State Grid Fujian Electric Power Co., Ltd.FuzhouChina
  4. 4.Environmental Futures Research Institute, School of Natural SciencesGriffith UniversityBrisbaneAustralia

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