Journal of Soils and Sediments

, Volume 19, Issue 4, pp 1959–1969 | Cite as

Evolution over years of structural characteristics of humic acids in Black Soil as a function of various fertilization treatments

  • Jiuming Zhang
  • Tingting AnEmail author
  • Fengqin Chi
  • Dan Wei
  • Baoku Zhou
  • Xiaoyu Hao
  • Liang Jin
  • Jingkuan WangEmail author
Soils, Sec 5 • Soil and Landscape Ecology • Research Article



Humic substances are the relatively stable and recalcitrant pool of soil organic carbon in the agricultural system. Humic acid (HA) is an active part of soil humic substances. However, little information exists on how structural characteristics of HA evolved with fertilization years in Black Soil.

Materials and methods

Soil samples were collected in 1997, 2002, 2008, and 2012 from the Long-term Fertilization Station of Black Soil. The methods of 13C-nuclear magnetic resonance spectroscopy, elemental composition analysis, and infrared spectroscopy were employed to analyze the structure of HA.

Results and discussion

Long-term fertilization had no significant (P > 0.05) effect on the ratio of O and S to C in HA. The ratio of H to C in HA increased with fertilization years after the application of organic manure (M) single or combined with chemical NPK fertilizers. The ratios of aliphatic C to aromatic C, and alkyl C to O-alkyl C in soil HA increased by 7.72% and 20.3%, respectively, in MNPK and M treatments, whereas NPK treatment declined the ratio of aliphatic C to aromatic C by 2.67% compared with CK treatment.


The HA structure tends to become aliphatic and simplified with years of organic manure combined with NPK fertilizers. To save costs, organic manure was only applied once within the period of crop rotation and was combined with NPK every year to satisfy crop demands for soil nutrients.


Black soil Humic acid Long-term fertilization Structural characteristics 


Funding information

This work was supported by the National Key Research and Development Program of China (2018YFD0200407, 2016YFD0300806), National Natural Science Foundation of China (41601247, 41771284), and the Key Fund of Heilongjiang Academy of Agricultural Sciences (2018KYJL011).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jiuming Zhang
    • 1
    • 2
  • Tingting An
    • 2
    Email author
  • Fengqin Chi
    • 1
  • Dan Wei
    • 3
  • Baoku Zhou
    • 1
  • Xiaoyu Hao
    • 1
  • Liang Jin
    • 1
  • Jingkuan Wang
    • 2
    Email author
  1. 1.Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Key Laboratory of Soil Environment and Plant Nutrition of Harbin, Institute of Soil and Fertilizer and Environment ResourcesHeilongjiang Academy of Agricultural SciencesHarbinChina
  2. 2.College of Land and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer ResourcesShenyang Agricultural UniversityShenyangChina
  3. 3.Beijing Academy of Agriculture and Forestry SciencesInstitute of Plant Nutrition and ResourcesBeijingChina

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