Evolution over years of structural characteristics of humic acids in Black Soil as a function of various fertilization treatments
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Humic substances are the relatively stable and recalcitrant pool of soil organic carbon in the agricultural system. Humic acid (HA) is an active part of soil humic substances. However, little information exists on how structural characteristics of HA evolved with fertilization years in Black Soil.
Materials and methods
Soil samples were collected in 1997, 2002, 2008, and 2012 from the Long-term Fertilization Station of Black Soil. The methods of 13C-nuclear magnetic resonance spectroscopy, elemental composition analysis, and infrared spectroscopy were employed to analyze the structure of HA.
Results and discussion
Long-term fertilization had no significant (P > 0.05) effect on the ratio of O and S to C in HA. The ratio of H to C in HA increased with fertilization years after the application of organic manure (M) single or combined with chemical NPK fertilizers. The ratios of aliphatic C to aromatic C, and alkyl C to O-alkyl C in soil HA increased by 7.72% and 20.3%, respectively, in MNPK and M treatments, whereas NPK treatment declined the ratio of aliphatic C to aromatic C by 2.67% compared with CK treatment.
The HA structure tends to become aliphatic and simplified with years of organic manure combined with NPK fertilizers. To save costs, organic manure was only applied once within the period of crop rotation and was combined with NPK every year to satisfy crop demands for soil nutrients.
KeywordsBlack soil Humic acid Long-term fertilization Structural characteristics
This work was supported by the National Key Research and Development Program of China (2018YFD0200407, 2016YFD0300806), National Natural Science Foundation of China (41601247, 41771284), and the Key Fund of Heilongjiang Academy of Agricultural Sciences (2018KYJL011).
- Dou S, Tan SW, Xu XC, Chen EF (1991) Effect of pig manure application on the structural characteristics of humic acid in brown soil. Pedosphere 1:345–354Google Scholar
- Foley WJ, McIlwee A, Lawler I, Aragones L, Woolnough AP, Berding N (1998) Ecological applications of near infrared reflectance spectroscopy - a tool for rapid, cost-effective prediction of the composition of plant and animal tissues and aspects of animal performance. Oecologia 116:293–305CrossRefGoogle Scholar
- Giovanela M, Crespo JS, Antunes M, Adamatti DS, Fernandes AN, Barison A, Silva d CWP, Guégan R, Motelica-Heino M, Sierra MMD (2010) Chemical and spectroscopic characterization of humic acids extracted from the bottom sediments of a Brazilian subtropical microbasin. J Mol Struct 981:111–119CrossRefGoogle Scholar
- Kononova MM (1961) Soil organic matter: its nature, its role in soil formation and in soil fertility. Oxford, New YorkGoogle Scholar
- Rossi QC, Pereira GM, García CA, Berbara LLR, Gazolla RP, Perin A, González PA (2016) Effects on the composition and structural properties of the humified organic matter of soil in sugarcane strawburning: a chronosequence study in the Brazilian Cerrado of Goiás state. Agric Ecosyst Environ 15:34–43CrossRefGoogle Scholar
- Stevenson FJ (1994) Humus chemistry: genesis, composition, reactions. Wiley, New YorkGoogle Scholar
- Stevenson FJ, He XT (1990) Nitrogen in humic substances as related to soil fertility. In: McCarthy P, Clapp CE, Malcolm RL, Bloom PR (eds) Humic Substances in Soil and Crop Sciences: Selected Readings. Soil Science Society of America and American Society of Agronomy, Madison, Wisconsin, pp 91–109Google Scholar
- Zhu Z, Jin J (2013) Fertilizer use and food security in China. Plant Nutr Fertil Sci 19:259–273 (in Chinese with English abstract)Google Scholar