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Nitrapyrin affects the abundance of ammonia oxidizers rather than community structure in a yellow clay paddy soil

  • Yan Gu
  • Wenhai Mi
  • Yinan Xie
  • Qingxu Ma
  • Lianghuan Wu
  • Zhaoping Hu
  • Feng Dai
Soils, Sec 5 • Soil and Landscape Ecology • Research Article
  • 12 Downloads

Abstract

Purpose

Yellow clay paddy soil (Oxisols) is a low-yield soil with low nitrogen use efficiency (NUE) in southern China. The nitrification inhibitor nitrapyrin (2-chloro-6- (tricholoromethyl)-pyridine, CP) has been applied to improve NUE and reduce environmental pollution in paddy soil. However, the effects of nitrapyrin combined with nitrogen fertilizers on ammonia oxidizers in yellow clay paddy soil have not been examined.

Materials and methods

A randomized complete block design was set with three treatments: (1) without nitrogen fertilizer (CK), (2) common prilled urea (PU), and (3) prilled urea with nitrapyrin (NPU). Soil samples were collected from three treatments where CK, PU, and NPU had been repeatedly applied over 5 years. Soil samples were analyzed by quantitative PCR and 454 high-throughput pyrosequencing of the amoA gene to investigate the influence of nitrapyrin combined with nitrogen on the abundance and community structure of ammonia oxidizers in yellow clay paddy soil.

Results and discussion

The potential nitrification rate (PNR) of the soil was significantly correlated with the abundances of both ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). Application of urea significantly stimulated AOA and AOB growth, whereas nitrapyrin exhibited inhibitory effects on AOA. Phylogenetic analysis showed that the most dominant operational taxonomic units (OTUs) of AOA and AOB were affiliated with the Nitrosotalea cluster and Nitrosospira cluster 12, respectively. AOA and AOB community structures were not altered by urea and nitrapyrin application.

Conclusions

Nitrogen fertilization stimulated nitrification and increased the population sizes of AOA and AOB. Nitrapyrin affected the abundance, but not community structure of ammonia oxidizers in yellow clay soil. Our results suggested that nitrapyrin improving NUE and inhibiting PNR was attributable to the inhibition of AOA growth.

Keywords

454 pyrosequencing Ammonia-oxidizing archaea Ammonia-oxidizing bacteria Nitrapyrin Paddy soil 

Notes

Acknowledgements

We would like to thank Editage for English language editing.

Funding

This work was supported by the National Key Research and Development Program of China (2016YFD0200102), the National Key Basic Research Program of China (2015CB150502), and the Key Research And Development Program of Zhejiang Province (2015C03011).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yan Gu
    • 1
    • 2
  • Wenhai Mi
    • 3
  • Yinan Xie
    • 1
    • 2
  • Qingxu Ma
    • 1
    • 2
  • Lianghuan Wu
    • 1
    • 2
    • 4
  • Zhaoping Hu
    • 4
  • Feng Dai
    • 5
  1. 1.Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, College of Environmental and Resource SciencesZhejiang UniversityHangzhouChina
  2. 2.Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource SciencesZhejiang UniversityHangzhouChina
  3. 3.College of Environmental Science and EngineeringYangzhou UniversityYangzhouChina
  4. 4.State Key Laboratory of Nutrition Resources Integrated UtilizationKingenta Ecological Engineering Group Co. Ltd.LinyiChina
  5. 5.Zhejiang Aofutuo Chemical Co. Ltd.ShangyuChina

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