Contribution of soil bacteria isolated from different regions into crude oil and oil product degradation
Crude oil and oil products are the most widespread environmental pollutants. The most efficient bioremediation is performed by using specific oil-degrading strains. Our objectives were to assess the role of soil bacteria, belonging to the following genera Arthrobacter, Microbacterium, Rhodococcus, Gordonia, and Acinetobacter in reduction of toxicity of environmental pollutants. Bacteria with different versatility were chosen: isolates from aromatic compounds or crude oil-contaminated soils and common representatives of the soil microflora.
Materials and methods
In this work, crude oil from the field Aschisay (Kazakhstan) of the following composition: alkanes 78%, naphthenes 6.7%, arenes 3.7%, and other compounds 11.6% was used as carbon source. To investigate the metabolic activity of microorganisms, they were cultured in flasks for 10 days under different conditions (variations in pH range, temperature, salinity, carbon source). Infrared spectrophotometry method was employed to determine the residual oil content after cultivation of bacteria. The ability of bacteria to produce biosurfactants was assessed by measuring surface tension and emulsifying activity (the Francey et al. method); localization of biosurfactants was detected.
Results and discussion
Forty-six strains from oil-spilled soils were isolated, with seven of these isolates showing the high degradation ability. Analysis of 16S-RNA gene sequences assigns these cultures to the genus Rhodococcus. Their degradation activity was then compared with the one of two rhodococci isolated from soil contaminated with chloroaromatics. The strains under study degraded crude oil, diesel fuel, and phenol; some of them destroyed benzene and naphthalene. The most active strains utilized up to 55–59% of crude oil hydrocarbons. The behavior of strains in the presence of petroleum components (benzene, toluene, nonane, decane, hexadecane) revealed bacterial persistence under severe conditions. Bacteria proved to be more sensitive to aromatic solvents than to aliphatic hydrocarbons. Most of the strains produced biosurfactants when grown on hydrophobic substrates.
The obtained results show that bacteria highly adapted to oil contaminations play an important role in the biodegradation of recalcitrant pollutants. Such strains may serve as the basis of bioaugmentation approach for soil remediation in sites with high contamination degree. Furthermore, this study highlights a significant role of common representatives of soil microflora in reducing pollution level in the soil owing to various, however, not necessary high destructive activities of soil strains.
KeywordsBacteria Contamination Degradation Oil hydrocarbons Soil
This work was supported by Russian Science Foundation (grant no. 14-14-00368) and Kazakhstanian–Russian project No. 142 “Development of a concept for monitoring contaminated soil in the Aral Sea region, and technologies for their remediation using new bioproducts.”
- Ausbel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1995) Short protocols in molecular biology, 3rd edn. John Wiley and Sons, NYGoogle Scholar
- Bello-Akinosho M, Makofane R, Adeleke R, Thantsha M, Pillay M, Chirima GJ (2016) Potential of polycyclic aromatic hydrocarbon-degrading bacterial isolates to contribute to soil fertility. Biomed Res Int, Article ID 5798593, doi: https://doi.org/10.1155/2016/5798593
- Carhart G, Hegeman G (1975) Improved method of selection for mutants of Pseudomonas putida. Appl Microbiol 30:1046–1047Google Scholar
- Chaudhary DK (2016) Bioremediation: an eco-friendly approach for polluted agriculture soil. Emer Life Sci Res 2:73–75Google Scholar
- Cirigliano M, Carman GM (1984) Isolation of bioemulsifier from Candida lipolytica. Appl Environ Microbiol 48:747–750Google Scholar
- Coronelli TV, Kalyuzhnaya ТВ (1983) Change in the ultrastructure of cells of saprotrophic mycobacteria under the influence of isoniazid. Microbiology 522(2):278–281Google Scholar
- Cui CZ, Zeng C, Wan X, Chen D, Zhang JY, Shen P (2008) Effect of rhamnolipids on degradation of anthracene by two newly isolated strains, Sphingomonas sp. 12A and Pseudomonas sp. 12B. J Microbiol Biotechnol 18(1):63–66Google Scholar
- de Carvalho CCCR (2010) Adaptation of Rhodococcus to organic solvents. In: Alvarez HM (ed) Biology of Rhodococcus. Springer-Verlag, Berlin, Heidelberg, pp 110–131Google Scholar
- Evans CGT, Herbert D, Tempest DB (1970) The continuous cultivation of microorganisms. 2. Construction of a chemostat. Meth Microbiol 2:277–327Google Scholar
- Gorlatov SN, Maltseva OV, Shevchenko VI, Golovleva LA (1989) Degradation of chlorophenols by a culture of Rhodococcus erythropolis. Mikrobiologiya (Moscow) 58:647–651Google Scholar
- Ivshina IB, Pshenichnov RA, Oborin AA (1987) Propane-oxidizing rodococci. Sverdlovsk, UNSC of the USSR Academy of Sciences (in Russian)Google Scholar
- Martínková L, Uhnáková B, Pátek M, Nešvera J, Křen V (2009) Biodegradation potential of the genus Rhodococcus. Environm Internation 35(1):162–177Google Scholar
- Plotnikova EG, Rybkina DO, Anan’ina LN, Yastrebova OV, Demakov VA (2006) Characterization of microorganisms isolated from technogenic soils of the Kama region. Russ J Ecol 4:261–268Google Scholar
- Sikkema J, de Bont JA, Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rew 59(2):201–222Google Scholar
- Solyanikova IP, Golovlev EL, Lisnyak OV, Golovleva LA (1999) Isolation and characterization of catechol 1,2-dioxygenases from Rhodococcus rhodnii strain 135 and Rhodococcus rhodochrous strain 89: comparison with analogous enzymes of the ordinary and modified ortho-cleavage pathways. Biokhimiya (Moscow) 64:824–831Google Scholar
- Solyanikova IP, Suzina NE, Egozarjan NS, Polivtseva VN, Mulyukin AL, Egorova DO, El-Registan GI, Golovleva LA (2017a) Structural and functional rearrangements in the cells of actinobacteria Microbacterium foliorum BN52 during transition from vegetative growth to a dormant state and during germination of dormant forms. Mikrobiologiya (Moscow) 86(4):463–475CrossRefGoogle Scholar
- Solyanikova IP, Suzina NE, Egozarjan NS, Polivtseva VN, Mulyukin AL, Egorova DO, El-Registan GI, Golovleva LA (2017b) The response of soil-dwelling Arthrobacter agilis Lush13 to stress impact: transition between vegetative growth and dormancy state. J Environm Sci Health Part B 52(10):745–751CrossRefGoogle Scholar
- Varjani SJ, Rana DP, Bateja S, Sharma MC, Upasani VN (2014) Screening and identification of biosurfactant (bioemulsifier) producing bacteria from crude oil contaminated sites of Gujarat, India. Int J Innovative Res Sci Eng Technol 3(2):9205–9213Google Scholar
- Zhukov DV, Murygina VP, Kalyuzhny SV (2006) Mechanisms of petroleum hydrocarbons degradation by microorganisms. Successes of modern. Biology 126(3):285–296Google Scholar