Journal of Soils and Sediments

, Volume 17, Issue 6, pp 1579–1587 | Cite as

Differential uptake of soluble organic and inorganic nitrogen by two fruit species: Dimocarpus longan Lour. and Eriobotrya japonica Lindl.

Soils, Sec 1 • Soil Organic Matter Dynamics and Nutrient Cycling • Research Article
First Online:
Received:
Accepted:
  • 155 Downloads

Abstract

Purpose

The aim of this study was to investigate whether two subtropical fruit species, Dimocarpus longan Lour. (Wulonglin) and Eriobotrya japonica Lindl. (Zaozhong 6), could directly take up soluble organic nitrogen (N), and if there was differential uptake of different forms of N between the two species.

Materials and methods

The stable isotopic tracing experiment was carried out using three 15N-labeled tracers [glycine-2-13C-15N, (15NH4)2SO4, and K(15NO3)] to examine the short-term (2, 6, and 72 h) uptake and distribution pattern of N within the plant in 1-year-old seedlings of both fruit species.

Results and discussion

After glycine-2-13C-15N application, the ratios of 13C to 15N concentration in the roots and whole seedlings of both Wulonglin and Zaozhong 6 were close to 1:1 at 2 and 6 h, respectively, showing that both fruit species could directly take up intact glycine. The 15NH4 + absorbed by Wulonglin and Zaozhong 6 whole seedlings in the (15NH4)2SO4 treatment were 13.2 and 9.2 times higher than glycine-derived-15N, while 15NO3 − absorbed in the K(15NO3) treatment were 27.7 and 7.7 times higher than glycine-derived-15N in the glycine-2-13C-15N treatment, respectively. This indicated that soluble organic N might not be the dominant N source taken up by both fruit species. Results also showed that Wulonglin preferred NO3 −, while Zaozhong 6 preferred NH4 +. The greater uptake of 15NH4 + and 15NO3 − than glycine-derived-15N by both fruit species might be related to their long-term adaptation to the supply of large quantity of inorganic N via fertilization. To elucidate the mechanisms responsible for the differential uptake of different forms of N by these two fruit species, further studies on mycorrhizal associations, transporter genes, and glycine metabolisms are warranted.

Conclusions

This study has demonstrated that both D. longan Lour. and E. japonica Lindl. seedlings were able to take up intact glycine directly, while the NO3 − and NH4 + were still dominant N forms absorbed by both fruit species.

Keywords

Dimocarpus longan Lour. Eriobotrya japonica Lindl. Glycine-2-13C-15N Isotope tracing Uptake 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgements

The authors would like to thank two anonymous referees for their critical comments, which have greatly improved the quality of this manuscript. This study was funded by the National Natural Science Foundation of China (40671086) and Provincial Natural Science Foundation of Fujian (2015J01090). The authors would like to thank the Fruit Research Institute, Fujian Academy of Agricultural Sciences, China, for providing Dimocarpus longan Lour (Wulonglin) and Eriobotrya japonica Lindl (Zaozhong 6) seedlings and Miss Xuemei Gao and Xiumei Chen for their assistance in soil sampling and the isotopic tracing experiment.

Supplementary material

11368_2016_1635_MOESM1_ESM.docx (35 kb)
ESM 1 (DOCX 34 kb)

References

  1. Bennett JN, Prescott CE (2004) Organic and inorganic nitrogen nutrition of western red cedar, western hemlock and salal in mineral N-limited cedar–hemlock forests. Oecologia 141: 468–476Google Scholar
  2. Britto DT, Kronzucker HJ (2002) NH4 + toxicity in higher plants: a critical review. J Plant Physiol. 159:567–584Google Scholar
  3. Bush DR (1993) Proton-Coupled Sugar and Amino Acid Transporters in Plants. Annu Rev Plant Physio 44:513–542Google Scholar
  4. Cao XC, Wu LH, Yuan L, Li XY, Zhu YH, Jin QY (2015) Uptake and uptake kinetics of nitrate, ammonium and glycine by pakchoi seedlings (Brassica campestris L. ssp. Chinensis L. Makino). Sci Hortic-Amsterdam 186:247–253Google Scholar
  5. Chapin FS, Moilanen L, Kielland K (1993) Preferential use of organic nitrogen for growth by a non-mycorrhizal arctic sedge. Nature 361:150–153CrossRefGoogle Scholar
  6. Choi WJ, Chang SX, Hao XY (2005) Soil retention, and tree uptake and tree resorption of 15NH4NO3 and NH4 15NO3 applied to trembling and hybrid aspens at planting. Can J For Res 35:823–831CrossRefGoogle Scholar
  7. Chen JZ (2011) Fruit cultivation (south version), 4th edition. China Agriculture Press, Beijing, pp 53–87Google Scholar
  8. Chen YT, Lai ZX, Chen JY, Zheng WL (2006) Advances in the studies of loquat germplasm. Subtropical Agri Res 2:5–10Google Scholar
  9. De Angeli A, Monachello D, Ephritikhine G, Frachisse JM, Thomine S, Gambale F, Barbier-Brygoo H (2006) The nitrate/proton antipoter AtClCa mediates nitrate accumulation in plant vacuoles. Nature 442:939–942Google Scholar
  10. Fischer WN, André B, Rentsch D, Krolkiewicz S, Tegeder M, Breitkreuz K, Frommer WB (1998) Amino acid transport in plants. Trends Plant Sci 3:188–195Google Scholar
  11. Ge TD (2008) A study on the characteristic of organic nitrogen uptake by tomato and the behavior of soluble organic nitrogen in soils. Shanghai Jiao Tong University 10:62–70Google Scholar
  12. Gao JQ, Mo Y, Xu XL, Zhang XW, Yu FH (2014) Spatiotemporal variations affect uptake of inorganic and organic nitrogen by dominate plant species in an alpine wetland. Plant Soil 381:21–278Google Scholar
  13. Gu XL, Li YP, Liang WH, Liu YQ, Dong DC (2008) The survey of longan industry development in China. Chinese Agri Sci Bulletin 24:470–474Google Scholar
  14. Hawkins HJ, Johansen A, George E (2000) Uptake and transport of organic and inorganic nitrogen by arbuscular mycorrhizal fungi. Plant Soil 226:275–285CrossRefGoogle Scholar
  15. Hawkins HJ, George E (2001) Reduce 15N-nitrogen transport through arbuscular mycorrhizal hyphae Triticum aestivum L. supplied with ammonium vs. nitrate nutrition. Botany 87:303–311CrossRefGoogle Scholar
  16. Jin VL, Evans RD (2010) Elevated CO2 increases plant uptake of organic and inorganic N in the desert shrub Larrea tridentata. Oecologia 163:257–266CrossRefGoogle Scholar
  17. Kahmen A, Livesley SJ, Arndt SK (2009) High potential but low actual glycine uptake of dominant plant species in three Australian land-use types with intermediate N availability. Plant Soil 325:109–121CrossRefGoogle Scholar
  18. Lipson DA, Monson RK (1998) Plant-microbe competition for soil amino acids in the alpine tundra: effects of freeze-thaw and dry-rewet events. Oecologia 113:406–414CrossRefGoogle Scholar
  19. Lipson DA, Näsholm T (2001) The unexpected versatility of plants: organic nitrogen use and availability in terrestrial ecosystems. Oecologia 128:305–361CrossRefGoogle Scholar
  20. Malagoli M, Dal Canal A, Quaggiotti S (2000) Differences in nitrate and ammonium uptake between Scots pine and European larch. Plant Soil 21:1–3Google Scholar
  21. Marschner H (1995) Mineral Nutrition of Higher Plants, 2nd edition, Academic Press, Amsterdam. pp231–255Google Scholar
  22. Matsumoto S, Ae N, Yamagata M (2000) Possible direct uptake of organic nitrogen from soil by chingensai (Brassica campestris L.) and carrot (Daucus carota L.). Soil Biol Biochem 32:1301–1310CrossRefGoogle Scholar
  23. Näsholm T, Ekblad A, Nordin A, Giesler R, Högberg M, Högberg P (1998) Boreal forest plants take up organic nitrogen. Nature 392:914–916CrossRefGoogle Scholar
  24. Näsholm T, Huss-Danell K, Högberg P (2001) Uptake of glycine by field grown wheat. New Phytol 150:59–63CrossRefGoogle Scholar
  25. Näsholm T, Kielland K, Ganeteg U (2009) Uptake of organic nitrogen by plants. New Phytol 12:31–48CrossRefGoogle Scholar
  26. Pfautsch S, Gessler A, Adams MA, Rennenberg H (2009) Using amino-N pools and fluxes to identify contributions of understorey Acacia spp. to overstorey Eucalyptus regnans and stand N uptake in temperate Australia. New Phytol 183:1097–1113CrossRefGoogle Scholar
  27. Rayment G, Higginson F (1992) Australian laboratory handbook of soil and water chemical methods. Inkata Press, MelbourneGoogle Scholar
  28. Schmidt SGR(1999) Glycine metabolism by plant roots and its occurrence in Australian plant communities. Aust J Plant Physiol 26:253–264Google Scholar
  29. Soil Survey Staff (1999) Soil Taxonomy. A basic system of soil classification for making and interpreting soil surveys, 2nd edition. Agricultural Handbook 436, Natural Resources Conservation Service, USDA, Washington DC, USAGoogle Scholar
  30. Taylor AFS, Gebauer G, Read DJ (2004) Uptake of nitrogen and carbon from double-labelled (N-15 and C-13) glycine by mycorrhizal pine seedlings. New Phytol 164:383–388CrossRefGoogle Scholar
  31. Tang XD (2007) Study on the arbuscular mycorrhizas and output predicting model of longan. Thesis for Master Degree, Fujian Normal University, pp 11–23Google Scholar
  32. Tang QY, Zhang CX (2013) Data Processing System (DPS) software with experimental design. Insect Sci 20:254–260Google Scholar
  33. Thornton B (2001) Uptake of glycine by non-mycorrhizal Lolium perenne. J Exp Bot 52:1315–1322Google Scholar
  34. Warren CR (2009) Uptake of inorganic and amino acid nitrogen from soil by Eucalyptus regnans and Eucalyptus pauciflora seedlings. Tree Physiol 29:401–409Google Scholar
  35. Wang WY, Ma YG, Xu J, Wang HC, Zhu JF, Zhou HK (2012) The uptake diversity of soil nitrogen nutrients by main plant species in Kobresia humilis alpine meadow on the Qinghai-Tibet Plateau. Sci China (Earth Science) 55:1688–1695CrossRefGoogle Scholar
  36. Wei LL, Chen CR, Xu ZH, Näsholm T (2013) Direct uptake and rapid decrease of organic nitrogen by Wollemia nobilis. Biol Fertil Soils 49:1247–1252CrossRefGoogle Scholar
  37. Weigelt A, Bol R, Bardgett RD (2005) Preferential uptake of soil nitrogen forms by grassland plant species. Oecologia 142:627–635CrossRefGoogle Scholar
  38. Wang XL (2006) A preliminary study on arbuscular mycorrhizas of loquat. Thesis for Master Degree, South China Agricultural University, pp 1–29Google Scholar
  39. Xu XL, Hua OY, Kuzyakov Y, Richter A, Wanek W (2006) Significance of organic nitrogen acquisition for dominant plant species in an alpine meadow on the Tibet plateau, China. Plant Soil 285:221–231CrossRefGoogle Scholar
  40. Tanaka Y, yano K (2005) Nitrogen delivery to maize via mycorrhizal hyphae depends on the form of N supplied. Plant Cell Environ 28:1247–1254CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.College of Resource and EnvironmentFujian Agriculture and Forestry UniversityFuzhouChina
  2. 2.Key Lab of Soil Environment Health and Regulation in FujianFujian Agriculture and Forestry UniversityFuzhouChina
  3. 3.Australian Rivers Institute, Griffith School of EnvironmentGriffith UniversityNathanAustralia

Personalised recommendations