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Journal of Soils and Sediments

, Volume 16, Issue 3, pp 831–841 | Cite as

Long-term electricity production from soil electrogenic bacteria and high-content screening of biofilm formation on the electrodes

  • Samuel Raj Babu Arulmani
  • Vimalan Jayaraj
  • Solomon RobinsonDavid JebakumarEmail author
Soils, Sec 2 • Global Change, Environ Risk Assess, Sustainable Land Use • Research Article

Abstract

Purpose

This study was conducted to determine the existence of soil bacteria in soil by soil microbial fuel cell (SMFC). The main objectives were (1) to differentiate the type of soil which will influence the electricity production, (2) to demonstrate the impact of different volume of soil in the MFC and feeding MFC for long-term electricity production, and (3) to conclude that electricity production is directly proportional to the biofilm formation on the anode surface.

Materials and methods

MudWatt kits were purchased from Keego Technologies USA, and 22 identical SMFCs were designed to study the electricity production from agricultural soil (S1) and vermicompost soil (S2). Ten milliliters of bioslurry is fed in SMFC to study the stability of electricity production at different stages. Microbes were isolated and characterized from the surface of the electrode. Biofilm analyses were done by high-content screening (HCS) system using 10 μl of acridine orange (100 μg/ml) at different stages of biofilm, and scanning electron microscopy is applied to confirm the matured biofilm on the surface of the anode.

Results and discussion

Application of bioslurry at different stages of electricity production conquers the normal energy recovery of the SMFCs and S2 soil with bioslurry sample produced the highest open circuit voltage (OCV) of 2.8 V (460 days) and S1 soil sample with bioslurry produced 1.7 V (364 days). The difference between SMFCs and MudWatt kits significantly confirms that increasing the volume of soil in the anode part increases the electricity production. The maximum OCV of S1 and S2 in MudWatt kits were 1.5 V (90 days) and 1.8 V (190 days), respectively. Increased volume of soil in our SMFCs produce maximum OCV of 1.8 V (S1 for 173 days) and 2.2 V (S2 for 240 days), and HCS analysis of biofilm at different stages reveals that electricity production is directly proportional to the biofilm formation.

Conclusions

Thus, it was concluded that the nature of soil and soil bacterium is important for the electricity production, and S2 soil sample produces maximum electricity than the S1 soil sample. Feeding of SMFCs with bioslurry aids the long-term and stabilized electricity production in both the soil samples.

Keywords

Biofilm Carbon fiber High-content screening Open-circuit voltage Soil microbial fuel cell 

Notes

Acknowledgments

This research work was supported by the Department of Biotechnology, India. The author is grateful to Mr. Keegan and the Keego Technologies LLC, Stanford, USA.

Supplementary material

ESM 1

A video of a Mini FAN motor in serial connections of MKU SMFCs (MP4 5385 kb)

11368_2015_1287_MOESM2_ESM.mp4 (7.2 mb)
ESM 2 A video of MudWatt Kit powering 1.5 V light emitting diode (LED) (MP4 7380 kb)

References

  1. Bond DR, Holmes DE, Tender LM, Lovley DR (2002) Electrode-reducing microorganisms that harvest energy from marine sediments. Science 295:483–485CrossRefGoogle Scholar
  2. Commault AS, Lear G, Novis P, Weld RJ (2014) Photosynthetic biocathode enhances the power output of a sediment-type microbial fuel cell. New Zeal J Bot 52(1):48–59CrossRefGoogle Scholar
  3. Daniel A, Leonard M, Tender J, Zeikus G, Park D, Lovley DR (2006) Harvesting energy from the marine sediment–water interface II kinetic activity of anode materials. Biosens Bioelectron 21:2058–2063CrossRefGoogle Scholar
  4. Davis R, Joseph S, Janssen H (2005) Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. Appl Environ Microbiol 71(2):826–834CrossRefGoogle Scholar
  5. Dulon S, Parot S, Délia M-L, Bergel A (2007) Electroactive biofilms: new means for electrochemistry. J Appl Electrochem 37:173–179CrossRefGoogle Scholar
  6. Dunaj SE, Joseph J, Vallino ME, Hines MG, Kobyljanec G, Juliette N, Varga R (2012) Relationships between soil organic matter, nutrients, bacterial community structure, and the performance of microbial fuel cells. Environ Sci Technol 46:1914–1922CrossRefGoogle Scholar
  7. Eulalio A, Mano M, Ferro MD, Zentilin L, Sinagra G, Zacchigna S, Giacca M (2012) Functional screening identifies miRNAs inducing cardiac regeneration. Nature 492:376–381CrossRefGoogle Scholar
  8. Ferrey S (2012) Earth, air, water and fire: the classical elements confront land and energy. J Land Use Envtl L 27:259Google Scholar
  9. Fullera ME, Kruczeka J, Schustera RL, Sheehanb PL, Arientic PM (2003) Bioslurry treatment for soils contaminated with very high concentrations of 2,4,6-trinitrophenylmethylnitramine (tetryl). J Hazard Mater 100(1–3):245–257CrossRefGoogle Scholar
  10. Govindarajan G, Santhi VS, Solomon RDJ (2014) Antimicrobial potential of phylogenetically unique actinomycete, Streptomyces sp. JRG-04 from marine origin. Biology 42:305–311CrossRefGoogle Scholar
  11. Huan D, Cheng WY, Fan Z, Chuan HZ, Zheng C, Juan HX, Feng Z (2014) Factors affecting the performance of single-chamber soil microbial fuel cells for power generation. Pedosphere 24(3):330–338CrossRefGoogle Scholar
  12. Huang L, Cheng S, Rezaei F, Logan BE (2009) Reducing organic loads in industrial effluents using microbial fuel cells. Environ Technol 30:499–504CrossRefGoogle Scholar
  13. Huang DY, Zhou SG, Chen Q, Zhao B, Yuan Y, Zhuang L (2011) Enhanced anaerobic degradation of organic pollutants in a soil microbial fuel cell. Chem Eng J 172:647–653CrossRefGoogle Scholar
  14. Huang Y, Zhen H, Kan J, Manohar AK, Nealson KH, Mansfeld F (2012) Electricity generation from a floating microbial fuel cell. Bioresour Technol 114:308–313CrossRefGoogle Scholar
  15. Ishii S, Hotta Y, Watanabe K (2008) Methanogenesis versus electrogenesis: morphological and phylogenetic comparisons of microbial communities. Biosci Biotechnol Biochem 72:286–294CrossRefGoogle Scholar
  16. Jose PA, Solomon RDJ (2012) Phylogenetic diversity of actinomycetes cultured from coastal multipond solar saltern in Tuticorin, India. Aquat Biosyst 8:23CrossRefGoogle Scholar
  17. Junyeong A, Bongkyu K, Jonghyeon N, How Y, Chang S (2013) Comparison in performance of sediment microbial fuel cells according to depth of embedded anode. Bioresour Technol 127:138–142CrossRefGoogle Scholar
  18. Logan BE, Call D, Cheng S, Hamelers HV, Sleutels TH, Jeremiasse AW, Rozendal RA (2010) Microbial electrolysis cells for high yield hydrogen gasproduction from organic matter. Environ Sci Technol 42:8630–8640CrossRefGoogle Scholar
  19. Lovley DR (2006) Bug juice: harvesting electricity with microorganisms. Nat Rev Microbiol 4:497–508CrossRefGoogle Scholar
  20. Lowy DA, Tender LM (2008) Harvesting energy from the marine sediment-water interface III. Kinetic activity of quinone- and antimony-based anode materials. J Power Sources 185(1):70–75CrossRefGoogle Scholar
  21. Patil SA, Harnisch F, Koch C, Hübschmann T, Fetzer I, Alessandro A, Martínez C, Müller S, Schroder U (2011) Electroactive mixed culture derived biofilms in microbial bioelectrochemical systems: the role of pH on biofilm formation, performance and composition. Bioresour Technol 102:9683–9690CrossRefGoogle Scholar
  22. Reimers CE, Leonard MT, Stephanie F, Wei W (2001) Harvesting energy from the marine sediment-water interface. Environ Sci Technol 35:192–195CrossRefGoogle Scholar
  23. Rezaei F, Richard TL, Brennan RA, Logan BE (2007) Substrate-enhanced microbial fuel cells for improved remote power generation from sediment based systems. Environ Sci Technol 41(11):4053–4058CrossRefGoogle Scholar
  24. Sajana TK, Ghangrekar M, Mitra A (2014) Effect of presence of cellulose in the freshwater sediment on the performance of sediment microbial fuel cell. Bioresour Technol 155:84–90CrossRefGoogle Scholar
  25. Samuel B, Jebakumar S, Prathipa R, Kumar M (2013) Production of electricity from agricultural soil and dye industrial effluent soil using microbial fuel cell. Int J Res Eng Tech 02(10):140–148CrossRefGoogle Scholar
  26. Sara DJ, Vallino JJ, Hines ME, Gay M, Kobyljanec C, Juliette N, Varga R (2012) Relationships between soil organic matter, nutrients, bacterial community structure, and the performance of microbial fuel cells. Environ Sci Technol 46:1914–1922CrossRefGoogle Scholar
  27. Singh A, Sharma RK, Agrawal M, Fiona M (2010) Health risk assessment of heavy metals via dietary intake of foodstuffs from the wastewater irrigated site of a dry tropical area of India. Food Chem Toxicol 48:611–619CrossRefGoogle Scholar
  28. Song S, Cai HT, Yan ZH, Zhao ZH, Jiang H (2012) Various voltage productions by microbial fuel cells with sedimentary inocula taken from different sites in one freshwater lake. Bioresour Technol 108:68–75CrossRefGoogle Scholar
  29. Torres CI, Brown K, Parameswaran P, Marcus P, Wanger AK, Gorby YA, Rittmann BE (2009) Selecting anode-respiring bacteria based on anode potential: phylogenetic, electrochemical, and microscopic characterization. Environ Sci Technol 43:9519–9524CrossRefGoogle Scholar
  30. Wang A, Cheng H, Ren N, Cui D, Lin N, Weimin W (2012) Sediment microbial fuel cell with floating biocathode for organic removal and energy recovery. Front Environ Sci Eng 6(4):569–574CrossRefGoogle Scholar
  31. Xu X, Zhao L, Wu MS (2014) Removal and changes of sediment organic matter and electricity generation by sediment microbial fuel cells and amorphous ferric hydroxide. Chem Biochem Eng Q 28(4):561–566CrossRefGoogle Scholar
  32. Yohann RT, Matthieu P, Arnaud C, Olivier B, Olivier S, Frédéric B (2013) A single sediment-microbial fuel cell powering a wireless telecommunication system. J Power Sources 241:703–708CrossRefGoogle Scholar
  33. Yuan Y, Zhou S, Zhuang L (2010) A new approach to in situ sediment remediation based on air-cathode microbial fuel cells. J Soils Sediments 10:1427–1433CrossRefGoogle Scholar
  34. Zhang M, Hongwei W (2015) Bioslurry as a fuel leaching characteristics of alkali and alkaline earth metallic species from biochar by bio-oil model compounds. Energy Fuels 29(4):2535–2541CrossRefGoogle Scholar
  35. Zhang T, Gannon SM, Nevin KP, Franks AE, Lovley DR (2010) Stimulating the anaerobic degradation of aromatic hydrocarbons in contaminated sediments by providing an electrode as the electron acceptor. Environ Microbiol 12(4):1011–1020CrossRefGoogle Scholar
  36. Zhao J, Li X, Ren Y, Wang X, Jian C (2012) Electricity generation from Taihu Lake cyanobacteria by sediment microbial fuel cells. J Chem Technol Biotechnol 87(11):1567–1573Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Samuel Raj Babu Arulmani
    • 1
  • Vimalan Jayaraj
    • 1
  • Solomon RobinsonDavid Jebakumar
    • 1
    Email author
  1. 1.Department of Molecular Microbiology, School of BiotechnologyMadurai Kamaraj UniversityMaduraiIndia

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