Journal of Soils and Sediments

, Volume 11, Issue 6, pp 990–999

Joint toxicity of three plant protection products to Triticum aestivum (L.) and Brassica rapa (L.)

  • Miguel João G. Santos
  • Amadeu M. V. M. Soares
  • Susana Loureiro
SOILS, SEC 4 • ECOTOXICOLOGY • RESEARCH ARTICLE

Abstract

Purpose

Few studies have been conducted to evaluate the effects of mixtures of chemicals in terrestrial environment. Thus, it seems important to evaluate if the combined application of pesticides currently used in agricultural fields may pose a risk to terrestrial plants.

Materials and methods

The effects of binary combinations of glyphosate, dimethoate, and spirodiclofen, applied to LUFA 2.2 soil, on the growth (determined following ISO guidelines) of the two species of plants (Brassica rapa and Triticum aestivum) were evaluated by the two reference models for mixture toxicity: concentration addition and independent action.

Results and discussion

Results of single exposure to the three pesticides indicated a decrease in shoot length and fresh weight of the two plant species only at the highest concentrations tested (approximately 54 times the recommended dose of glyphosate, 37 times the recommended dose of dimethoate, and 22 times the recommended dose of spirodiclofen). Synergism was only found in the combination of glyphosate and dimethoate when the independent action model was fitted to the shoot length and fresh weight of T. aestivum.

Conclusions

The results obtained in the present work seem to corroborate that these pesticides have no detrimental effects when applied at recommended doses. The joint toxicity effect of the pesticides was mainly antagonistic.

Keywords

Fresh biomass Higher plants Mixture toxicity Pesticides Shoot growth 

References

  1. Abo-El-Seod MA, Frost M (1998) Biochemical changes in wheat plants as affected by residues of dimethoate and pirimicarb. Environ Manag Health 9:188–193CrossRefGoogle Scholar
  2. An Y-J, Lee W-M (2007) Decreased toxicity to terrestrial plants associated with a mixture of methyl tert-butyl ether and its metabolite tert-butyl alcohol. Environ Toxicol Chem 26:1711–1716CrossRefGoogle Scholar
  3. Andersen ME, Dennison JE (2004) Mechanistic approaches for mixture risk assessments—present capabilities with simple mixtures and future directions. Environ Toxicol Pharm 16:1–11CrossRefGoogle Scholar
  4. Biediger DL, Baumann PA, Weaver DN, Chandler JM, Merkle MG (1992) Interactions between primisulfuron and selected soil-applied insecticides in corn (Zea mays). Weed Technol 6:807–812Google Scholar
  5. Bielecki K, Sarapuk J, Kleszczyńska H (2004) Binary mixtures of (n-phosphonomethyl)-glycine with new aminophosphonates. Z Naturforsch 59:515–518Google Scholar
  6. Blackburn LG, Boutin C (2003) Subtle effects of herbicide use in the context of genetically modified crops: a case study with glyphosate (Roundup). Ecotoxicology 12:271–285CrossRefGoogle Scholar
  7. Bliss CI (1939) The toxicity of poisons applied jointly. Ann Appl Biol 26:585–615CrossRefGoogle Scholar
  8. Cedergreen N, Streibig JC (2005) Can the choice of endpoint lead to contradictory results of mixture-toxicity experiments? Environ Toxicol Chem 24:1676–1683CrossRefGoogle Scholar
  9. Chopra SI, Nandra KS (1969) Effect of thiometon on the germination of sarson (Brassica campestris Linn. var Brown sarson). J Agr Food Chem 17:805–806CrossRefGoogle Scholar
  10. Chowdhury ABMNU, Jepson PJ, Ford MG, Frampton GK (2005) The role of cuticular waxes and surface roughness in determining the insecticidal efficacy of deltamethrin and dimethoate applied as emulsifiable concentrates to leaf surfaces. Int J Pest Manag 51:253–263CrossRefGoogle Scholar
  11. De Jong FMW, De Snoo GR, Van de Zande JC (2008) Estimated nationwide effects of pesticide spray drift on terrestrial habitats in the Netherlands. J Environ Manag 86:721–730CrossRefGoogle Scholar
  12. De Zwart D, Posthuma L (2005) Complex mixture toxicity for single and multiple species: proposed methodologies. Environ Toxicol Chem 24:2665–2676CrossRefGoogle Scholar
  13. Dekker JH, Chandler K (1985) Herbicide effect on the viability of quackgrass (Agropyron repens) rhizome buds. Can J Plant Sci 65:1057–64CrossRefGoogle Scholar
  14. Eurostat (2007) The use of plant protection products in the European Union. Data 1992–2003. Eurostat Statistical books, 2007 edn. European Communities, LuxembourgGoogle Scholar
  15. Glass RL (1987) Adsorption of glyphosate by soils and clay minerals. J Agric Food Chem 35:497–500CrossRefGoogle Scholar
  16. Greco W, Unkelbach HD, Pöch G, Sühnel J, Kundi M, Bodeker W (1992) Consensus on concepts and terminology for combined-action assessment: the Saariselkä agreement. Arch Complex Environ Studies 4:65–69Google Scholar
  17. Greco WR, Bravo G, Parsons JC (1995) The search for synergy: a critical review from a response durface perspective. Pharmacol Rev 47:332–385Google Scholar
  18. Hanley ME, Whiting MD (2005) Insecticides and arable weeds: effects on germination and seedling growth. Ecotoxicology 14:483–490CrossRefGoogle Scholar
  19. Hardman JM, Franklin JL, Moreau DL, Bostanian NJ (2003) An index for selective toxicity of miticides to phytophagous mites and their predators on orchard trials. Pest Manag Sci 59:1324–1332CrossRefGoogle Scholar
  20. ISO (1995) Soil quality—Determination of the effects of pollutants on soil flora—Part 2: Effects of chemicals on the emergence and growth of higher plants. ISO/DIS 11269–2. International Organization for Standardization Geneve, SwitzerlandGoogle Scholar
  21. ISO (2004) Soil quality—biological methods—chronic toxicity in higher plants., ISO/DIS 22030, International Organization for Standardization, Geneva, SwitzerlandGoogle Scholar
  22. Jonker MJ, Svendsen C, Bedaux JJM, Bongers M, Kammenga JM (2005) Significance testing of synergistic/antagonistic, dose level-dependent, or dose ratio-dependent effects in mixture dose-response analysis. Env Toxicol Chem 24:2701–2713CrossRefGoogle Scholar
  23. Kalsch W, Junker T, Römbke J (2006) A chronic plant test for the assessment of contaminated soils. Part 1: method development. J Soils Sediments 6:37–45CrossRefGoogle Scholar
  24. Loewe S, Muischnek H (1926) Effect of combinations: mathematical basis of problem. N-S Arch Ex Path Ph 114:313–326CrossRefGoogle Scholar
  25. Long SM, Reicheinberg F, Lister LJ, Hankard PM, Townsend J, Mayer P, Wright J, Holmstrup M, Svendsen C, Spurgeon DJ (2009) Combined chemical (fluoranthene) and drought effects on Lumbricus rubellus demonstrate the applicability of the independent action model for multiple stressor assessment. Environ Toxicol Chem 28:629–636CrossRefGoogle Scholar
  26. Loureiro S, Amorim MJB, Campos B, Rodrigues SMG, Soares AMVM (2009) Assessing joint toxicity of chemicals in Enchytraeus albidus (Enchytraeidae) and Porcellionides pruinosus (Isopoda) using avoidance behaviour as an endpoint. Environ Pollut 157:625–632CrossRefGoogle Scholar
  27. Loureiro S, Svendsen C, Ferreira ALG, Pinheiro C, Ribeiro F, Soares AMVM (2010) Toxicity of three binary mixtures to Daphnia magna: comparing chemicals mode of action and deviations from conceptual models. Environ Toxicol Chem 29:1716–1276CrossRefGoogle Scholar
  28. Martikainen ETP, Krogh PH (1999) Effects of soil organic matter content and temperature on toxicity of dimethoate to Folsomia fimetaria (Collembola: Isotomiidae). Environ Toxicol Chem 18:865–872Google Scholar
  29. Martin ML, Ronco AE (2006) Effect of mixtures of pesticides used in the direct seeding technique on nontarget plant seeds. Bull Environ Contam Toxicol 77:228–236Google Scholar
  30. Matzke M, Stolte S, Böschen A, Filser J (2008) Mixture effects and predictability of combination effects in imidazolium based ionic liquids as well as imidazolium based ionic liquids and cadmium on terrestrial plants (Triticum aestivum) and limnic green algae (Scenedesmus vacuolatus). Green Chem 10:784–792CrossRefGoogle Scholar
  31. Munkegaard M, Abbaspoor M, Cedergreen N (2008) Organophosphorous insecticides as herbicide synergists on the green algae Pseudokirchneriella subcapitata and the aquatic plant Lemna minor. Ecotoxicology 17:29–35Google Scholar
  32. Munshi SK, Kumari A, Sanadi S, Bakhetia DRC, Sukhija PS (1987) Influence of systemic insecticides on lipid biosynthesis in seeds of Indian mustard (Brassica juncea L.). Plant Sci 53:201–208CrossRefGoogle Scholar
  33. Nauen R (2005) Spirodiclofen: mode of action and resistance risk assessment in Tetranychid pest mites. J Pestic Sci 30:272–274Google Scholar
  34. Ogg AG Jr, Young FL (1991) Effects of preplant treatment interval and tillages on herbicide toxicity to winter wheat (Triticum aestivum). Weed Tech 5:291–296Google Scholar
  35. Olszyk D, Pfleeger T, Lee HE, Plocher M (2010) Phytotoxicity assay for seed production using Brassica rapa L. Integrated Environ Assess Manag 6:725–734CrossRefGoogle Scholar
  36. Petersen IL, Hansen HCB, Ravn HW, Sørensen JC, Sørensen H (2007) Metabolic effects in rapeseed (Brassica napus, L.) seedlings after root exposure to glyphosate. Pest Biochem Physiol 89:220–229CrossRefGoogle Scholar
  37. Raudomis L (2006) Comparative toxicity of spirodiclofen and lambdacihalotrin to Tetranychus urticae, Tarsonemus pallidus and predatory mite Amblyseius andersoni in a strawberry site under field conditions. Agron Res 4:317–322Google Scholar
  38. Reddy KN, Rimando AM, Duke SO, Nandula VK (2008) Aminomethylphosphonic acid accumulation in plant species treated with glyphosate. J Agric Food Chem 56:2125–2130CrossRefGoogle Scholar
  39. Ribo JM (1986) “Roundup” (Glyphosate): a TRC technical review. Toxicology Research Centre, University of Saskatchewan, SaskatchewanGoogle Scholar
  40. Santos MJG, Soares AMVM, Loureiro S (2010) Joint effects of three plant protection products to the terrestrial isopod Porcellionides pruinosus and the collembolan Folsomia candida. Chemosphere 80:1021–1030CrossRefGoogle Scholar
  41. Shepherd J, Arthur E (2002) Mobility Determination of BAJ2740 by Soil Thin-Layer Chromatography in Four Soils: Lab Project Number: BJ182101: 110343. Unpublished study prepared by Bayer Corporation. 54 pGoogle Scholar
  42. Soltani N, Shropshire C, Sikkema PH (2009) Sensitivity of winter wheat to preplant and preemergence glyphosate tankmixes. Crop Prot 28:449–452CrossRefGoogle Scholar
  43. Song NH, Yin XL, Chen GF, Yang H (2007) Biological responses of wheat (Triticum aestivum) plants to the herbicide chlorotoluron in soils. Chemosphere 68:1779–1787CrossRefGoogle Scholar
  44. Sprankel P, Meggitt WF, Penner P (1975) Adsorption, mobility, and microbial degradation of glyphosate by soils. Weed Sci 23:229–234Google Scholar
  45. Spurgeon DJ, Jones OA, Dorne JL, Svendsen C, Swain S, Stürzenbaum SR (2010) Systems toxicology approaches for understanding the joint effects of environmental chemical mixtures. Sci Total Environ 408:3725–2734CrossRefGoogle Scholar
  46. Steinrucken HC, Amrhein N (1980) The herbicide glyphosate is a potent inhibitor of 5-enolylpyruvyl-shikimic acid-3-phosphate. Biochem Biophys Res Comm 94:1207–1212CrossRefGoogle Scholar
  47. Turgut C, Formin A (2002) Sensitivity of the rooted macrophyte Myryophyllum aquaticum (Vell.) Verdcourt to seventeen pesticides determined on the basis of EC50. Bull Environ Contam Toxicol 69:601–608CrossRefGoogle Scholar
  48. Vieira MM (2009) Vendas de produtos fitofarmacêuticos em Portugal em 2008. Direcção Geral de Agricultura e Desenvolvimento Rural. Ministério da Agricultura e do Desenvolvimento Rural, PortugalGoogle Scholar
  49. Wachendorff U, Nauen R, Schnorbach HJ, Rauch N, Elbert A (2002) The biological profile of Spirodiclofen (Envidor®)—a new selective tetronic acid acaricide. Bayer Crop Sci J 73:149–176Google Scholar
  50. Wolf C, Schnorbach H-J (2002) Ecobiological profile of the acaricide spirodiclofen. Bayer Crop Sci J 55:177–906Google Scholar
  51. Xiang W-S, Wang X-J, Ren T-R, Ju X-L (2005) Expression of a wheat cytochrome P450 monooxygenase in yeast and its inhibition by glyphosate. Pest Manag Sci 61:402–406CrossRefGoogle Scholar
  52. Yenish JP, Young F (2000) Effect of preharvest glyphosate application on seed and seedling quality of spring wheat (Triticum aestivum). Weed Tech 14:212–217CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Miguel João G. Santos
    • 1
  • Amadeu M. V. M. Soares
    • 1
  • Susana Loureiro
    • 1
  1. 1.Departamento de Biologia and CESAMUniversidade de AveiroAveiroPortugal

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