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Phytoremediation for soils contaminated by phenanthrene and pyrene with multiple plant species

  • ISMESS 2009 * RESEARCH ARTICLE
  • Published:
Journal of Soils and Sediments Aims and scope Submit manuscript

Abstract

Purpose

The remediation of soil polluted by polycyclic aromatic hydrocarbons (PAHs) is of great importance due to the persistence and carcinogenic properties of PAHs. Phytoremediation has been regarded as a promising alternative among suggested approaches. For the establishment of highly effective remediation method and better understanding of the remediation mechanisms by plants, the potentials of three plant species and their planting patterns on the remediation efficacy were studied by pot experiments.

Materials and methods

Soils were amended with phenanthrene (Phe) or pyrene (Pyr) at five levels ranging from 0∼322 mg kg–1, then incubated for 30 days. At each level of PAHs, four treatments were set as: (a) treatments 1, no plant and microbe-inhibited with addition of 0.1%NaN3; (b) treatments 2, no plant and without the addition of NaN3; (c) treatments 3, microbe-inhibited and planted; (d) treatments 4, planted and without inhibition of microbes. For each planted treatment, single cropping of rape (Medicago sativa), alfalfa (Brassica campestris), and white clover (Trifolium repens), and mixed cropping of rape with alfalfa and white clover were adapted. Seedlings of 7 days old were then cultivated. After 70 days cultivation, soil and plant of each treatment were sampled for analyses of PAHs.

Results and discussion

Rape, alfalfa, and white clover all significantly promoted the degradation of PAHs in soils; alfalfa and white clover showed higher efficiencies for the removal of PAHs. Averagely, about 41.46% of Phe or 33.69% of Pyr were removed from soils after 70-day plantation of alfalfa, and 38.75% of Phe or 36.29% of Pyr removed by white clover, as compared to the much lower degradation rates of 22.57% of Phe or 18.24% of Pyr for non-planted controls. Mixed cropping significantly enhanced the remediation efficiencies as compared to single cropping; about 74.87% of Phe or 62.81% of Pyr were removed by mixed cropping of rape and alfalfa, and 72.01% of Phe or 68.44% of Pyr by mixed cropping of rape and white clover.

Conclusions

The presence of vegetation significantly promoted the dissipation of Phe or Pyr in soils and remediation efficiency varied greatly among plant species and cropping patterns. Rape had the lowest ability for the removal of PAHs, while alfalfa showed highest ability for the remediation of Phe and white clover was most effective for Pyr. Mixed cropping of rape with alfalfa or white clover was however far better for the remediation of soil PAHs than single cropping.

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References

  • Anderson TA, Guthrie EA, Walton BT (1993) Bioremediation in the rhizosphere. Environ Sci Technol 27:2630–2637

    Article  CAS  Google Scholar 

  • Chen YC, Banks MK, Schwab AP (2003) Pyrene degradation in the rhizosphere of Tall Fescue (Festuca arundinacea) and Switch grass (Panicum virgatum L.). Environ Sci Technol 37:5778–5782

    Article  CAS  Google Scholar 

  • Child R, Miller CD, Liang Y, Narasimham G, Chatterton J, Sims RC, Britt D, Anderson AJ (2007a) Polycyclic aromatic hydrocarbon-degrading Mycobacterium isolates: their association with plant roots. Appl Microbiol Biotechnol 75:655–663

    Article  CAS  Google Scholar 

  • Child R, Miller CD, Liang Y, Sims RC, Anderson AJ (2007b) Pyrene mineralization by Mycobacterium sp strain KMS in a Barley rhizosphere. J Environ Qual 36(5):1260–1265

    Article  CAS  Google Scholar 

  • Chiou CT, Sheng GY, Manes M (2001) A partition-limited model for plant uptake of organic contaminants from soil and water. Environ Sci Technol 35:1437–1444

    Article  CAS  Google Scholar 

  • Corgie SC, Joner EJ, Leyval C (2003) Rhizospheric degradation of phenanthrene is a function of proximity to roots. Plant Soil 257(1):143–150

    Article  CAS  Google Scholar 

  • Daniele F, Ivano V, Sun CG, Colin E, Snape CM, Anthony EF (2004) Source apportionment of polycyclic aromatic hydrocarbons in acoastal lagoon by molecular and isotopic characterization. Mar Chem 84:123–135

    Google Scholar 

  • Gao YZ, Zhu LZ (2004) Plant uptake, accumulation and translocation of phenanthrene and pyrene in soils. Chemosphere 55:1169–1178

    Article  CAS  Google Scholar 

  • Gao YZ, Zhu LZ (2005) Phytoremediation for phenanthrene and pyrene contaminated soils. J Environ Sci (China) 17(1):14–18

    CAS  Google Scholar 

  • Gerhardt KE, Huang XD, Glick BR, Greenberg BM (2009) Phytoremediation and rhizoremediation of organic soil contaminants: potential and challenges. Plant Sci 176:20–30

    Article  CAS  Google Scholar 

  • Haritash AK, Kaushik CP (2009) Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. J Hazard Mater 169:1–15

    Article  CAS  Google Scholar 

  • Huang XD, El-Alawi Y, Donna MP, Bernard RG, Bruce MG (2004) Responses of three grass species to creosote during phytoremediation. Environ Pollut 130:453–463

    Article  CAS  Google Scholar 

  • Jennifer LK, John NK, Hung L, Jack TT (2005) The effects of perennial ryegrass and alfalfa on microbial abundance and diversity in petroleum-contaminated soil. Environ Pollut 133:455–465

    Article  CAS  Google Scholar 

  • Joner EJ, Leyval C (2003) Rhizosphere gradients of polycyclic aromatic hydrocarbon (PAH) dissipation in two industrial soils and the impact of arbuscular mycorrhiza. Environ Sci Technol 37:2371–2375

    Article  CAS  Google Scholar 

  • Khan AA, Wang RF, Cao WW, Doerge DR, Wennerstrom D, Cerniglia CE (2001) Molecular cloning, nucleotide sequence, and expression of genes encoding a polycyclic aromatic ring dioxygenase from Mycobacterium sp strain PYR-1. Appl Microbiol Biotechnol 67:3577–3585

    CAS  Google Scholar 

  • Khan S, Hesham A, Gu Q, Liu S, He JZ (2009) Biodegradation of pyrene and catabolic genes in contaminated soils cultivated with Lolium multiflorum L. J Soils Sediments 9:482–491

    Article  CAS  Google Scholar 

  • Kipoponlou AM, Manoli E, Samara C (1999) Bioconcentration of polycyclic aromatic hydrocarbons in vegetables grown in an industrial area. Environ Pollut 106:369–380

    Article  Google Scholar 

  • Lee SH, Lee WS, Lee CH, Kim JG (2008) Degradation of phenanthrene and pyrene in rhizosphere of grasses and legumes. J Hazard Mater 153:892–898

    Article  CAS  Google Scholar 

  • Lewis HS, Schwab AP, Banks MK (2001) Phytoremediation of aged petroleum sludge: effect of irrigation techniques and scheduling. J Environ Qual 30:1516–1522

    Article  Google Scholar 

  • Liliana G, Maria AR (2004) Potential of extra cellular enzymes in remediation of polluted soils: a review. Enzyme Microb Technol 35:339–354

    Article  CAS  Google Scholar 

  • Liste HH, Alexander M (2000) Plant-promoted pyrene degradation in soil. Chemosphere 40:7–10

    Article  CAS  Google Scholar 

  • Macek T, Mackova M, Kas J (2000) Research review paper exploitation of plants for the removal of organics in environmental remediation. Biotechnol Adv 18:23–34

    Article  CAS  Google Scholar 

  • Maila MP, Randima P, Cloete TE (2005) Multispecies and monoculture rhizoremediation of polycyclic aromatic hydrocarbons (PAHs) from the soil. Int J Phytoremediat 7(2):87–98

    Article  CAS  Google Scholar 

  • Menzie CA, Potocki BB, Santodonato J (1992) Exposure to carcinogenic PAHs in the environment. Environ Sci Technol 26:1278–1284

    Article  CAS  Google Scholar 

  • Muratova A, Hübner T, Tischer S, Turkovskaya O, Möder M, Kuschk P (2003) Plant-rhizosphere-microflora association during phytoremediation of PAH-contaminated soil. Int J Phytoremediat 5(2):137–151

    Article  CAS  Google Scholar 

  • Pan SW, Wei SQ, Yuan X, Cao SX (2008a) Mechanisms of the removal and remediation of phenanthrene and pyrene in soil by dwarf lilyturf. Acta Ecologica Sinica (in Chinese) 28(8):3654–3661

    CAS  Google Scholar 

  • Pan SW, Wei SQ, Yuan X, Cao SX (2008b) The removal and remediation of phenanthrene and pyrene in soil by mixed cropping of alfalfa and rape. Agri Sci China 7(11):101–110

    Google Scholar 

  • Paterson S, Mackay D (1994) A model of organic chemical uptake by plants from soil and the atmosphere. Environ Sci Technol 28:2259–2265

    Article  CAS  Google Scholar 

  • Parrish ZD, Banks MK, Schwab AP (2005) Assessment of contaminant lability during phytoremediation of polycyclic aromatic hydrocarbons impacted soil. Environ Pollut 137:187–197

    Article  CAS  Google Scholar 

  • Reilley KA, Banks MK, Schwab AP (1996) Dissipation of polycyclic aromatic hydrocarbons in the rhizosphere. J Environ Qual 25:212–218

    CAS  Google Scholar 

  • Ryan K, Miya MK (2001) Enhanced phenanthrene biodegradation in soil by slender oat root exudates and root debris. J Environ Qual 30:1911–1918

    Article  Google Scholar 

  • Schnoor JL, Licht LA, McCutcheon SC, Wolfe NL, Carreira LH (1995) Phytoremediation of organic and nutrient contaminants. Environ Sci Technol 29:318–323

    Article  Google Scholar 

  • Siciliano S, Germida JJ, Banks K, Greer CW (2003) Changes in microbial community composition and function during a polyaromatic hydrocarbon phytoremediation field trial. Appl Environ Microbiol 69:483–489

    Article  CAS  Google Scholar 

  • Tsao DT (2003) Overview of phytotechnologies. Adv Biochem Eng Biotech 78:4–50

    Google Scholar 

  • Toner EJ, Lceyval C (2003) Rhizosphere gradients of polycyclic aromatic hydrocarbons (PAHs) dissipation in two industrial soil and the impact of arhuscular mycorrhiza. Environ Sci Technol 37:2371–2375

    Article  CAS  Google Scholar 

  • Walton BT, Anderson TA (1988) Structural properties of organic chemicals as predictors of biodegradation and microbial toxicity in soils. Chemosphere 17:1501–1507

    Article  Google Scholar 

  • Wang M, Jones KC (1994) Uptake of chlorobenzencs by carrots from spiked and sewage sludge-amended soil. Environ Sci Technol 28:1260–1267

    Article  CAS  Google Scholar 

  • Wang YX, Oyaizua H (2009) Evaluation of the phytoremediation potential of four plant species for dibenzofuran-contaminated soil. J Hazard Mater 168:760–764

    Article  CAS  Google Scholar 

  • Wilson SC, Jones KC (1993) Remediation of soil contaminated with polynuclear aromatic hydrocarbons (PAHs): a review. Environ Pollut 81:229–249

    Article  CAS  Google Scholar 

  • Xu SY, Chen YX, Wu WX, Wang KX, Lin Q, Liang XQ (2006) Enhanced dissipation of phenanthrene and pyrene in spiked soils by combined plants cultivation. Sci Total Environ 363:206–215

    Article  CAS  Google Scholar 

  • Yi H, Crowley DE (2007) Biostimulation of PAH degradation with plants containing high concentrations of linoleic acid. Environ Sci Technol 41(12):4382–4388

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was financially supported by the National Scientific and Technological Supporting Program of China (2007BAD87B10-5), and by the “211” Ecology National Key Discipline of Southwest University.

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Authors and Affiliations

  1. Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education in China), College of Resources and Environment, Southwest University, Chongqing, 400716, People’s Republic of China

    Shiqiang Wei & Shengwang Pan

  2. Key Laboratory of Agricultural Resources and Environment in Chongqing, Chongqing, 400716, People’s Republic of China

    Shiqiang Wei & Shengwang Pan

  3. Environment Science and Engineering Institute of Chengdu University, Chengdu, 610106, China

    Shengwang Pan

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  1. Shiqiang Wei
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  2. Shengwang Pan
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Correspondence to Shiqiang Wei.

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Responsible editor: Jianming Xu

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Wei, S., Pan, S. Phytoremediation for soils contaminated by phenanthrene and pyrene with multiple plant species. J Soils Sediments 10, 886–894 (2010). https://doi.org/10.1007/s11368-010-0216-4

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  • DOI: https://doi.org/10.1007/s11368-010-0216-4

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