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Can mouth part deformities of Chironomus riparius serve as indicators for water and sediment pollution? A laboratory approach

Abstract

Purpose

The significance of chironomids mouthpart deformities as suitable indicators for pollutant contamination of natural waters and sediments has been investigated and discussed for several decades. Uncertainties still exist as further laboratory studies, with different pollutants and with the same experimental design are required.

Materials and methods

In this study, the effects of four substances (i.e., nickel chloride, chlorpyrifos, imidacloprid and thiacloprid) were tested on the mouthpart deformity rates and patterns in Chironomus riparius. These compounds were investigated either individually or in mixtures.

Results and discussion

No significant increase in the frequency of mouthpart deformities was found using different single substance treatments when compared to the controls. Consequently no concentration–effect relationships between substance concentration and deformity frequency were detected. In mixture experiments an increase in mouthpart deformities of C. riparius exposed to imidacloprid–thiacloprid mixtures was detected. This indicated that the effects of single substances and mixtures on mouthpart deformity frequency may differ considerably.

Conclusions

The findings in this study from different laboratory approaches in combination with the published literature questions the reliability of chironomids mouthpart deformities as indicators of freshwater and sediment contamination by toxic substances.

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References

  1. Ankley GT, Call DJ, Cox JS, Kahl MD, Hoke RA, Kosian PA (1994) Organic carbon partitioning as a basis for predicting the toxicity of chlorpyrifos in sediments. Environ Toxicol Chem 13(4):621–626

  2. Bailey HC, Deanovic L, Reyes E, Kimball T, Larson K, Cortright K, Connor V, Hinton DE (2000) Diazinon and chlorpyrifos in urban waterways in Northern California, USA. Environ Toxicol Chem 19(1):82–87

  3. Belden JB, Lydy MJ (2000) Impact of atrazine on organosphosphate insecticide toxicity. Environ Toxicol Chem 19(9):2266–2274

  4. Bhattacharyay G, Sadhu AK, Mazumdar A, Chaudhuri PK (2005) Antennal deformities of chironomid larvae and their use in biomonitoring of heavy metal pollutants in the river Damodar of West Bengal, India. Environ Monit Assess 108(1):67–84

  5. Bird GA (1994) Use of chironomid deformities to assess environmental degradation in the Yamaska River, Quebec. Environ Monit Assess 30(2):163–175

  6. Bird GA, Schwartz WJ, Joseph DL (1995) The effect of 210 PB and stable lead on the induction of menta deformities in Chironomus tentas larvae and on their growth and suvival. Environ Toxicol Chem 14(12):2125–2130

  7. Bleeker EAJ, Leslie HA, Groenendijk D, Plans M, Admiraal W (1999) Effects of exposure to azaarenes on emergence and mouthpart development in the midge Chironomus riparius (Diptera: Chironomidae). Environ Toxicol Chem 18(8):1829–1834

  8. Callaghan A, Hirthe G, Fisher T, Crane M (2001) Effect of short-term exposure to chlorpyrifos on developmental parameters and biochemical biomarkers in Chironomus riparius Meigen. Ecotox Environ Safe 50(1):19–24

  9. Chau YK, Kulikovsky-Cordeiro OTR (1995) Occurrence of nickel in the Canadian environment. Environ Rev 3(1):95–120

  10. Dermott RM (1991) Deformities in larval Procladius spp. and dominant chironomini from the St. Clair River. Hydrobiologia 219(1):171–185

  11. Dias V, Vasseur C, Bonzom J-M (2008) Exposure of Chironomus riparius larvae to uranium: effects on survival, development time, growth, and mouthpart deformities. Chemosphere 71(3):574–581

  12. Dickman M, Rygiel G (1996) Chironomid larval deformity frequencies, mortality, and diversity in heavy-metal contaminated sediments of a Canadian riverine wetland. Environ Int 22(6):693–703

  13. Dow AgroSciences (2008) About chlorpyrifos: In: LLC DA, Dow AgroSciences LLC (ed)

  14. Escher BI, Hermens JLM (2002) Modes of action in ecotoxicology: their role in body burdens, species sensitivity, QSARs, and mixture effects. Environ Sci Technol 36(20):4201–4217

  15. Fisher TC, Crane M, Callaghan A (2000) An optimized microtiterplate assay to detect acetylcholinesterase activity in individual Chironomus riparius Meigen. Environ Toxicol Chem 19(7):1749–1752

  16. Fowlkes MD, Michael JL, Crisman TL, Prenger JP (2003) Effects of the herbicide imazapyr on benthic macroinvertebrates in a logged pond cypress dome. Environ Toxicol Chem 22(4):900–907

  17. Gerhardt A, Bisthoven LJ (1995) Behavioural, developmental and morphological responses of Chironomus gr. thummi larvae (Diptera, Nematocera) to aquatic pollution. Aquat Ecosys Stress Recovery (Formerly Journal of Aquatic Ecosystem Health) 4(3):205–214

  18. Gerhardt A, Janssens de Bisthoven L (2006) Life cycle test of aquatic stages of Chironomus riparius exposed to Ni, Chlorpyrifos alone and in combination. SETAC Europe Annual Meeting 2006. The Hague, The Netherlands, p 1

  19. Gerhardt A, Orendt C, Dettinger-Klemm A, Janssens de Bisthoven L, Michiels S, Otto C-J, Vogt C (2006) Chironomiden: potential und Einsatzmöglichkeiten im Biomonitoring von Gewässern—ein Überblick. DGL-Mitteilungen I:49–57

  20. Gilliom RJ, Barbash JE, Crawford CG, Hamilton PA, Martin JD, Nakagaki N, Nowell LH, Scott JC, Stackelberg PE, Thelin GP et al (2006) Pesticides in the nation’s streams and ground water, 1992–2001: the quality of our nation’s waters. Report nr 1-411-30955-3. p 172

  21. Groenendijk D, Postma J, Kraak M, Admiraal W (1998) Seasonal dynamics and larval drift of Chironomus riparius (Diptera) in a metal contaminated lowland river. Aquat Ecol 32(4):341–351

  22. Hämäläinen H (1999) Critical appraisal of the indexes of chironomid larval deformities and their use in bioindication. Ann Zool Fenn 36:179–186

  23. Janssens De Bisthoven L, Nuyts P, Goddeeris B, Ollevier F (1998a) Sublethal parameters in morphologically deformed Chironomus larvae: clues to understanding their bioindicator value. Freshwater Biol 39(1):179–191

  24. Janssens de Bisthoven L, Vermeulen A, Ollevier F (1998b) Experimental induction of morphological deformities in Chironomus riparius larvae by chronic exposure to copper and lead. Arch Environ Contam Toxicol 35(2):249–256

  25. Janssens de Bisthoven L, Postma J, Vermeulen A, Goemans G, Ollevier F (2001) Morphological deformities in Chironomus riparius Meigen larvae after exposure to cadmium over several generations. Water Air Soil Poll 129(1):167–179

  26. Jemec A, Tišler T, Drobne D, Sepčić K, Fournier D, Trebše P (2007) Comparative toxicity of imidacloprid, of its commercial liquid formulation and of diazinon to a non-target arthropod, the microcrustacean Daphnia magna. Chemosphere 68:1408–1418

  27. Jeschke P, Nauen R (2008) Neonicotinoids from zero to hero in insecticide chemistry. Pest Manag Sci 64:1084–1098

  28. Jeyasingham K, Ling N (1997) Head capsule deformities in Chironomus zealandicus (Diptera: Chironomidae), influence of site and substrate. New Zeal J Mar Fresh 31:175–184

  29. Jeyasingham K, Ling N (2000) Seasonal influence on head capsule deformities in Chironomus zealandicus (Hudson) (Diptera: Chironomidae). Hydrobiologia 427(1):75–82

  30. Jonker MJ, Svendsen C, Bedaux JJM, Bongers M, Kammenga JE (2005) Significance testing of synergistic/antagonistic, dose level-dependent, or dose ratio-dependent effects in mixture dose-response analysis. Environ Toxicol Chem 24(10):2701–2713

  31. Kasprzak KS (1987) Nickel. Adv Mod Environ Toxicol 2:145–183

  32. Kwak IS, Lee W (2005) Mouthpart deformity and developmental retardation exposure of Chironomus plumosus (Diptera: Chironomidae) to tebufenozide. Bull Environ Contam Toxicol 75(5):859–865

  33. Langer-Jaesrich M, Köhler H-R, Gerhardt A (2009) Assessing the toxicity of the insecticide thiacloprid on Chironomus riparius (Insecta: Diptera) using multiple endpoints. Arch Environ Contam Toxicol .online available doi: 10.1007/s00244-009-9420-x

  34. Lydy MJ, Belden JB, Ternes MA (1999) Effects of temperature on the toxicity of m-parathion, chlorpyrifos, and pentachlorobenzene to Chironomus tentans. Arch Environ Contam Toxicol 37(4):542–547

  35. Madden C, Suter P, Nicholson B, Austin A (1992) Deformities in chironomid larvae as indicators of pollution (pesticide) stress. Aquat Ecol 26(2):551–557

  36. Maienfisch P, Haettenschwiler J, Rindlisbacher A, Decock A, Wellmann H, Kayser H (2003) Azido-neonicotinoids as candidate photoaffinity probes for insect nicotinic acetylcholine receptors. Int J Chem 57:710–714

  37. Martinez EA, Moore BC, Schaumloffel J, Dasgupta N (2001) Induction of morphological deformities in Chironomus tentans exposed to zinc-and lead-spiked sediments. Environ Toxicol Chem 20(11):2475–2481

  38. Martinez EA, Moore BC, Schaumloffel J, Dasgupta N (2003) Morphological abnormalities in Chironomus tentans exposed to cadmium-and copper-spiked sediments. Ecotox Environ Safe 55(2):204–212

  39. Matthiessen P (2008) An assessment of endocrine disruption in mollusks and the potential for developing internationally standardized mollusk life cycle test guidelines. Integr Environ Assess Manag 4(3):274–284

  40. Meregalli G, Ollevier F (2001) Exposure of Chironomus riparius larvae to 17[alpha]-ethynylestradiol: effects on survival and mouthpart deformities. Sci Total Environ 269(1–3):157–161

  41. Meregalli G, Vermeulen AC, Ollevier F (2000) The use of chironomid deformation in an in situ test for sediment toxicity. Ecotox Environ Safe 47(3):231–238

  42. Meregalli G, Pluymers L, Ollevier F (2001) Induction of mouthpart deformities in Chironomus riparius larvae exposed to 4-n-nonylphenol. Environ Poll 111(2):241–246

  43. Moore MT, Huggett DB, Gillespie JWB, Rodgers JJH, Cooper CM (1998) Comparative toxicity of chlordane, chlorpyrifos, and aldicarb to four aquatic testing organisms. Arch Environ Contam Toxicol 34(2):152–157

  44. Nazarova L, Riss H, Kahlheber A, Werding B (2004) Some observations of buccal deformities in chironomid larvae (Diptera: Chironomidae) from the Ciénaga Grande de Santa Marta, Colombia. Caldasia 26(1):275–290

  45. OECD (2004a) OECD Guidelines for the testing of chemicals 218: sediment-water chironomid toxicity test using spiked sediment. p 21

  46. OECD (2004b) OECD Guidelines for the testing of chemicals 219: sediment-water chironomid toxicity test using spiked water. p 21

  47. Oehlmann J, Schulte-Oehlmann U, Tillmann M, Markert B (2000) Effects of endocrine disruptors on prosobrach snails (Mollusca: Gastropoda) in the laboratory. Part I: Bisphenol A und Octylphenol as xeno-estrogens. Ecotoxicology 9(6):383–397

  48. Pfeuffer RJ, Matson F (2001) Pesticide surface water quality report: March 2001 sampling event. South Florida Water Management District, USA

  49. Postma JF, Davids C (1995) Tolerance induction and life cycle changes in cadmium-exposed Chironomus riparius (Diptera) during consecutive generations. Ecotox Environ Safe 30(2):195–202

  50. Reynolds S, Ferrington L (2001) Temporal and taxonomic patterns of mouthpart deformities in larval midges (Diptera: Chironomidae) in relation to sediment chemistry. J Freshwater Ecol 16(1):15–27

  51. Richardson RJ (1995) Assessment of the neurotoxic potential of chlorpyrifos relative to other organophosphorus compounds: a critical review of the literature. J Toxicol Environ Health 44(2):135–165

  52. Schulz R (2001) Rainfall-induced sediment and pesticide input from orchards into the Lourens River, Western Cape, South Africa: importance of a single event. Water Res 35(8):1869–1876

  53. Servia M, Cobo F, Gonzalez MA (1998) Deformities in larval Prodiamesa olivacea (Meigen, 1818) (Diptera, Chironomidae) and their use as bioindicators of toxic sediment stress. Hydrobiologia 385:153–162

  54. Servia M, Cobo F, González M (2000) Seasonal and interannual variations in the frequency and severity of deformities in larvae of Chironomus riparius (Meigen, 1804) and Prodiamesa olivacea (Meigen, 1818) (Diptera, Chironomidae) collected in a polluted site. Environ Monit Assess 64(3):617–626

  55. Süß A, Bischoff G, Mueller A, Buhr L (2006) Chemisch-biologisches Monitoring zu Pflanzenschutzmittelbelastungen und Lebensgemeinschaften in Gräben des Alten Landes. Nachrichtenbl Deut Pflanzenschutzd 58:28–42

  56. Urk Gv, Kerkurm F, Smit H (1992) Life cycle patterns, density, and frequency of deformities in chironomus larvae (Diptera: Chironomidae) over a contaminated sediment gradient. Can J Fish Aquat Sci 49(11):2291–2299

  57. Vedamanikam V, Shazili N (2009) Observations of mouthpart deformities in the chironomus larvae exposed to different concentrations of nine heavy metals. Toxicol Environ Chem 91(1):57–63

  58. Vermeulen A, Dall P, Lindegaard C, Ollevier F, Goddeeris B (1998) Improving the methodology of chironomid deformation analysis for sediment toxicity assessment: a case study in three Danish lowland streams. Arch Hydrobiol 144(1):103–125

  59. Vermeulen AC, Liberloo G, Dumont P, Ollevier F, Goddeeris B (2000a) Exposure of Chironomus riparius larvae (diptera) to lead, mercury and [beta]-sitosterol: effects on mouthpart deformation and moulting. Chemosphere 41(10):1581–1591

  60. Vermeulen AC, Liberloo G, Ollevier F, Goddeeris B (2000b) Ontogenesis, transfer and repair of mouthpart deformities during moulting in Chironomus riparius (Diptera, Chironomidae). Arch Hydrobiol 147(3):401–415

  61. Warwick WF (1990) Morphological deformities in chironomidae (Diptera) larvae from the Lac St. Louis and Laprairie Basins of the St. Lawrence River. J Great Lakes Res 16(2):185–208

  62. Watts MM, Pascoe D, Carroll K (2003) Exposure to 17[alpha]-ethinylestradiol and bisphenol A—effects on larval moulting and mouthpart structure of Chironomus riparius. Ecotoxicol Environ Saf 54(2):207–215

  63. Welshons W, Thayer K, Judy B, Taylor J, Curran E, Saal FSv (2003) Large effects from small exposures. I. Mechanisms for endocrine-disrupting chemicals with estrogenic activity. Environ Health Perspect 111(8):994–1006

  64. Wiederholm T (1984) Incidence of deformed chironomid larvae (Diptera:Chironomidae) in Swedish lakes. Hydrobiologia 109:243–249

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Acknowledgements

The study was supported by the EU Integrated Project NoMiracle (Novel Methods for Integrated Risk assessment of Cumulative Stressors in Europe; http://nomiracle.jrc.it) contract No. 003956 under the EU-theme ‘Global Changes and Ecosystems’ topic ‘Development of risk assessment methodologies’, coordinated by Hans Løkke at NERI, DK-8600 Sikeborg, Denmark, granted to Almut Gerhardt, LimCo International.

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Correspondence to Miriam Langer-Jaesrich.

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Responsible editor: Klara Hilscherova

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Langer-Jaesrich, M., Köhler, H. & Gerhardt, A. Can mouth part deformities of Chironomus riparius serve as indicators for water and sediment pollution? A laboratory approach. J Soils Sediments 10, 414–422 (2010). https://doi.org/10.1007/s11368-010-0195-5

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Keywords

  • Chironomids
  • Mixture effects
  • Mouthpart deformities
  • Pollutant indicator