Chronic toxicity of contaminated sediments on reproduction and histopathology of the crustacean Gammarus fossarum and relationship with the chemical contamination and in vitro effects
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The aim of the present study was to investigate possible relationships between the sediment contaminants and the occurrence of intersex in situ. Two of the studied sediments were from polluted sites with increased occurrence of intersex crustaceans (Lake Pilnok, black coal mining area in the Czech Republic, inhabited by the crayfish Pontastacus leptodactylus population with 18% of intersex; creek Lockwitzbach in Germany with Gammarus fossarum population with about 7% of intersex).
Materials and methods
Sediments were studied by a combined approach that included (1) determination of concentrations of metals and traditionally analyzed organic pollutants such as polychlorinated biphenyls, pesticides, and polycyclic aromatic hydrocarbons (PAHs); (2) examination of the in vitro potencies to activate aryl hydrocarbon (AhR), estrogen (ER), and androgen receptor-mediated responses; and (3) in vivo whole sediment exposures during a 12-week reproduction toxicity study with benthic amphipod G. fossarum.
Results and discussion
Investigations showed that Lake Pilnok was highly contaminated by powdered waste coal, contained high concentrations of PAHs (up to 12 μg/g dry weight), and exhibited various effects in biotests (high concentrations of AhR and ER agonists were determined by in vitro assays with H4IIE.luc cells and yeast luciferase reporter gene assays). Less pronounced effects were observed in Lockwitzbach and Steinlach creek sediments. Long-term in vivo laboratory exposures with G. fossarum resulted in significant mortalities and sex-specific toxicities (reflected in hepatopancreas histopathology). Significant effects on the reproduction-related parameters were observed at Lake Pilnok sediments, which elevated numbers of newly hatched individuals and stimulated reproduction cycle in females (larger portions of mature oocytes in comparison to other variants).
Results of the present study indicate that sediments from Lake Pilnok contain a large portion of dioxin-like, estrogenic, and anti-androgenic compounds, which stimulated fecundity in G. fossarum. Although some effects might be attributed to PAHs, most of the bioactive compounds could not be detected by traditional instrumental analyses. Possibly, bioavailable fractions of the maceral (solid coal mass rich in organic compounds) could have contributed to the observed activities, but only few studies investigated its biological effects, and it will require further research. The present study emphasizes the need for integrated assessment of contaminated sediments to elucidate their ecotoxicological impacts.
KeywordsAndrogenicity Estrogenicity Fecundity In vitro Reproduction toxicity Sediments
The research was supported by the Czech Ministry of Education (the INCHEMBIOL framework project MSM0021622412 and project ENVISCREEN 2B08036). The authors greatly acknowledge Dr. Marko Virta (University of Helsinki, Finland, for providing us with the yeast cell lines) and Dr. Zdeněk Ďuriš (University of Ostrava, Czech Republic, for initial inputs to the investigation). A scholarship of E.M. was provided by Deutsche Bundesstiftung Umwelt (DBU, Germany).
- ASTM (1979) Annual book of ASTM standards. Part 26—gaseous fuels, coal and coke, atmospheric analysis. American Society for Testing and Materials, PhiladelphiaGoogle Scholar
- FDEP (2003) Development and evaluation of numerical sediment quality assessment guidelines for Florida inland waters. Florida Department of Environmental Protection, TallahasseeGoogle Scholar
- Harrison FW (1992) Crustacea. In: Harrison FW, Humes AG (eds) Microscopic anatomy of invertebrates. Wiley-Liss, New York, pp 529–617Google Scholar
- Jungmann D, Köhler A, Köhler H-R, Ladewig V, Licht O, Ludwichowski K-U, Schirling M, Triebskorn R, Nagel R. (2004a) Umweltchemikalien mit Wirkung auf das Hormonsystem—TV 5: Wirkung von Xenohormonen in aquatischen Ökosystemen. Report nr F + E-Vorhaben 299 65 221/05, German Federal Environmental Agency (UBA), BerlinGoogle Scholar
- Kizu R, Okamura K, Toriba A, Kakishima H, Mizokami A, Burnstein KL, Hayakawa K (2003) A role of aryl hydrocarbon receptor in the antiandrogenic effects of polycyclic aromatic hydrocarbons in LNCaP human prostate carcinoma cells. Arch Toxicol 77:335–343Google Scholar
- Millennium Ecosystem Assessment (2005) Ecosystems and human well-being: Wetlands and water. Synthesis. World Resources Institute, WashingtonGoogle Scholar
- Richardson KC, Jarret L, Finke EH (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technology 35:313–325Google Scholar
- Sanchez P, Alonso C, Fernandez C, Vega MM, Garcia MP, Tarazona JV (2005) Evaluation of a multi-species test system for assessing acute and chronic toxicity of sediments and water to aquatic invertebrates—effects of pentachlorophenol on Daphnia magna and Chironomus prasinus. J Soils Sediments 5(1):53–58CrossRefGoogle Scholar
- Schill RO, Görlitz H, Köhler H-R (2003) Laboratory stimulation of a mining accident: acute toxicity, hsc/hsp70 response, and recovery from stress in Gammarus fossarum (Crustacea, Amphipoda) exposed to a pulse of cadmium. Biol Metal 16:391–401Google Scholar
- Schirling M, Triebskorn R, Köhler HR (2004) Variation in stress protein levels (hsp70 and hsp90) in relation to oocyte development in Gammarus fossarum (Koch 1835). Invertebr Reprod Dev 45:161–167Google Scholar
- Watts MM, Pascoe D, Carroll K (2002) Population responses of the freshwater amphipod Gammarus pulex (L.) to an environmental estrogen, 17 alpha-ethinylestradiol. Environ Toxicol Chem 21:445–450Google Scholar