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Journal of Soils and Sediments

, Volume 10, Issue 3, pp 423–433 | Cite as

Chronic toxicity of contaminated sediments on reproduction and histopathology of the crustacean Gammarus fossarum and relationship with the chemical contamination and in vitro effects

  • Edita Mazurová
  • Klára Hilscherová
  • Tereza Šídlová-Štěpánková
  • Heinz-R. Köhler
  • Rita Triebskorn
  • Dirk Jungmann
  • John P. Giesy
  • Luděk Bláha
SEDIMENTS, SEC 3 • SEDIMENT MANAGEMENT AT THE RIVER BASIN SCALE • RESEARCH ARTICLE

Abstract

Purpose

The aim of the present study was to investigate possible relationships between the sediment contaminants and the occurrence of intersex in situ. Two of the studied sediments were from polluted sites with increased occurrence of intersex crustaceans (Lake Pilnok, black coal mining area in the Czech Republic, inhabited by the crayfish Pontastacus leptodactylus population with 18% of intersex; creek Lockwitzbach in Germany with Gammarus fossarum population with about 7% of intersex).

Materials and methods

Sediments were studied by a combined approach that included (1) determination of concentrations of metals and traditionally analyzed organic pollutants such as polychlorinated biphenyls, pesticides, and polycyclic aromatic hydrocarbons (PAHs); (2) examination of the in vitro potencies to activate aryl hydrocarbon (AhR), estrogen (ER), and androgen receptor-mediated responses; and (3) in vivo whole sediment exposures during a 12-week reproduction toxicity study with benthic amphipod G. fossarum.

Results and discussion

Investigations showed that Lake Pilnok was highly contaminated by powdered waste coal, contained high concentrations of PAHs (up to 12 μg/g dry weight), and exhibited various effects in biotests (high concentrations of AhR and ER agonists were determined by in vitro assays with H4IIE.luc cells and yeast luciferase reporter gene assays). Less pronounced effects were observed in Lockwitzbach and Steinlach creek sediments. Long-term in vivo laboratory exposures with G. fossarum resulted in significant mortalities and sex-specific toxicities (reflected in hepatopancreas histopathology). Significant effects on the reproduction-related parameters were observed at Lake Pilnok sediments, which elevated numbers of newly hatched individuals and stimulated reproduction cycle in females (larger portions of mature oocytes in comparison to other variants).

Conclusions

Results of the present study indicate that sediments from Lake Pilnok contain a large portion of dioxin-like, estrogenic, and anti-androgenic compounds, which stimulated fecundity in G. fossarum. Although some effects might be attributed to PAHs, most of the bioactive compounds could not be detected by traditional instrumental analyses. Possibly, bioavailable fractions of the maceral (solid coal mass rich in organic compounds) could have contributed to the observed activities, but only few studies investigated its biological effects, and it will require further research. The present study emphasizes the need for integrated assessment of contaminated sediments to elucidate their ecotoxicological impacts.

Keywords

Androgenicity Estrogenicity Fecundity In vitro Reproduction toxicity Sediments 

Notes

Acknowledgements

The research was supported by the Czech Ministry of Education (the INCHEMBIOL framework project MSM0021622412 and project ENVISCREEN 2B08036). The authors greatly acknowledge Dr. Marko Virta (University of Helsinki, Finland, for providing us with the yeast cell lines) and Dr. Zdeněk Ďuriš (University of Ostrava, Czech Republic, for initial inputs to the investigation). A scholarship of E.M. was provided by Deutsche Bundesstiftung Umwelt (DBU, Germany).

Supplementary material

11368_2009_166_MOESM1_ESM.doc (56 kb)
Supplementary Table 1 Proportion of intersex individuals in natural populations of Gammarus fossarum from Steinlach Creek and Lockwitzbach Creek and among animals after 12-week laboratory exposures to the four studied sediments (DOC 56 kb)
11368_2009_166_MOESM2_ESM.doc (56 kb)
Supplementary Table 2 The classification criteria for hepatopancreas histology of Gammarus fossarum. The impairment of three different criteria was evaluated on a four-graded scale (DOC 56 kb)
11368_2009_166_MOESM3_ESM.doc (57 kb)
Supplementary Table 3 Concentrations of toxic metals in sediments from investigated sites (DOC 57 kb)
11368_2009_166_MOESM4_ESM.doc (80 kb)
Supplementary Fig. 1 The size distribution of individuals of Gammarus fossarum found in the natural population ((a) in total, 62 males and 50 females examined) and at the end of 12 weeks of exposures to control sediment (b), sediment from the Lockwitzbach Creek (c), and sediment from Lake Pilnok (d, e). Presented are means (±standard deviation) of cephalothorax size of males, females and juveniles/subadults ((b, e) four replicate aquaria; (c, d) three replicate aquaria) (DOC 80 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Edita Mazurová
    • 1
  • Klára Hilscherová
    • 1
  • Tereza Šídlová-Štěpánková
    • 1
  • Heinz-R. Köhler
    • 2
  • Rita Triebskorn
    • 2
    • 3
  • Dirk Jungmann
    • 4
  • John P. Giesy
    • 5
    • 6
    • 7
    • 8
  • Luděk Bláha
    • 1
  1. 1.Faculty of Science, RECETOX, Research Centre for Environmental Chemistry and EcotoxicologyMasaryk UniversityBrnoCzech Republic
  2. 2.Animal Physiological EcologyUniversity of TübingenTübingenGermany
  3. 3.Steinbeis-Transfer Center for Ecotoxicology and EcophysiologyRottenburgGermany
  4. 4.Institute of HydrobiologyDresden University of TechnologyDresdenGermany
  5. 5.Department of Veterinary Biomedical Sciences and Toxicology CentreUniversity of SaskatchewanSaskatoonCanada
  6. 6.Zoology Department, National Food Safety and Toxicology Center, and Center for Integrative ToxicologyMichigan State UniversityEast LansingUSA
  7. 7.Biology and Chemistry DepartmentCity University of Hong KongKowloon, Hong KongChina
  8. 8.School of the EnvironmentNanjing UniversityNanjingChina

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