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  • SOILS, SEC 2 • GLOBAL CHANGE, ENVIRON RISK ASSESS, SUSTAINABLE LAND USE • RESEARCH ARTICLE
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Does history matter? Temperature effects on soil microbial biomass and community structure based on the phospholipid fatty acid (PLFA) analysis

Abstract

Background, aim, and scope

Temperature is an important environmental factor regulating soil microbial biomass, activity, and community. Soils from different climatic regions may have very different dominant soil microbes, which are acclimated to the local conditions like temperature. Changing soil temperature especially warming has been shown to increase the mortality rate of soil microbes. However, little is known about the responses of soil microbes coming from different climatic regions to different incubation temperatures. The objective of this study was to examine the temperature effects on microbial biomass and community of soils collected from cold, intermediate, and hot natural sites.

Materials and methods

Soils were collected from northern (Heilongjiang province), central (Jiangsu province), and southern (Guangxi province) China, these soils having very different temperature histories. The Heilongjiang soil was from the coldest region with a mean annual temperature of 1.2°C, the Jiangsu soil was intermediate with a mean annual temperature of 15.7°C, and Guangxi soil was from the hottest area, with a mean annual temperature of 21.2°C. These three soils were incubated at 4°C, 15°C, 25°C, and 35°C for up to 56 days. Phospholipid fatty acid (PLFA) analyses were conducted on days 0, 3, 7, 14, 28, and 56 to track the dynamics of soil microbes.

Results

Soil microbial biomass indexed by total phospholipid fatty acid concentration decreased with increasing incubation temperature, with that of the Heilongjiang soil decreasing most. At the end of incubation, the biomass at 35°C in the Heilongjiang, Jiangsu, and Guangxi soils had declined to 65%, 72%, and 96% of the initial biomass, respectively. The PLFA patterns shifted with increasing temperatures in all the soils, especially at 35°C; the change was biggest in the Heilongjiang soil.

Discussion

History does have effects on soil microbes responding to environmental stress. Soil microbial biomass and PLFA profiles shifted least in the Guangxi soil with the hottest temperature history and most in the Heilongjiang soil with the coldest temperature, indicating that the distribution of free-living microorganisms is influenced by climatic factors. The majority of soil microorganisms coming from the hot regions are more adapted to high temperature (35°C) compared to those from the cold area. There are some regular changes of PLFA profiles when increasing incubation temperature to 35°C. However, the effect of incubation temperature on soil microbial community structure was inconclusive. As PLFA profile community structure is the phenotypic community structure. Genotype experiments are required to be done in future studies for the better understanding of soil microbes in different climate regions with concerning temperature variation.

Conclusions

With the increasing incubation temperature, soil microbial biomass and PLFA profiles shifted most in the soil with the coldest temperature history and least in the soil with the hottest temperature. History does matter in determining soil microbial dynamics when facing thermal stress.

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References

  1. Agricultural Chemistry Committee of China (1983) Conventional methods of soil and agricultural chemistry analysis. Science, Beijing (In Chinese)

  2. Bausenwein U, Gattinger A, Langer U, Embacher A, Hartmann H-P, Sommer M, Munch JC, Schloter M (2008) Exploring soil microbial communities and soil organic matter: variability and interactions in arable soils under minimum tillage practice. Appl Soil Ecol 40:67–77

  3. Cho JC, Tiedje JM (2000) Biogeography and degree of endemicity of fluorescent Pseudomonas strains in soil. Appl Environ Microbiol 66:5448–5456

  4. Fierer N, Jackson RB (2006) The diversity and biogeography of soil bacterial communities. PNAS 103:626–631

  5. Frostegård Å, Tunlid A, Bååth E (1993) Phospholipid fatty acid composition, biomass, and activity of microbial communities from two soil types experimentally exposed to different heavy metals. Appl Environ Microbiol 59:3605–3617

  6. Grayston SJ, Campbell CD, Bardgett RD, Mawdsley JL, Clegg CD, Ritz K, Griffiths BS, Rodwell JS, Edwards SJ, Davies WJ, Elston DJ, Millard P (2004) Assessing shifts in microbial community structure across a range of grasslands of differing management intensity using CLPP, PLFA and community DNA techniques. Appl Soil Ecol 25:63–84

  7. Grisi B, Grace C, Brookes PC, Benedetti A, Dell’abate MT (1998) Temperature effects on organic matter and microbial biomass dynamics in temperate and tropical soils. Soil Biol Biochem 30:1309–1315

  8. He Y, Xu J, Ma Z, Wang H, Wu Y (2007) Profiling of PLFA: implications for nonlinear spatial gradient of PCP degradation in the vicinity of Lolium perenne L. roots. Soil Biol Biochem 39:1121–1129

  9. He Y, Xu J, Lv X, Ma Z, Wu J, Shi J (2009) Does the depletion of pentachlorophenol in root-soil interface follow a simple linear dependence on the distance to root surgaces? Soil Biol Biochem . doi:10.1016/j.soilbio.2008.11.016

  10. Hill GT, Mitkowski NA, Aldrich-Wolfe L, Emele LR, Jurkonie DD, Ficke A, Maldonado-Ramirez S, Lynch ST, Nelson EB (2000) Methods for assessing the composition and diversity of soil microbial communities. Appl Soil Ecol 15:25–36

  11. Jeffery S, Harris JA, Rickson RJ, Ritz K (2007) Microbial community phenotypic profiles change markedly with depth within the first centimetre of the arable soil surface. Soil Biol Biochem 39:1226–1229

  12. Joergensen RG, Brookes PC, Jenkinson DS (1990) Survival of the soil microbial biomass at elevated temperatures. Soil Biol Biochem 22:1129–1136

  13. Kieft TL, Ringelberg DB, White DC (1994) Changes in ester-linked phospholipid fatty acid profiles of subsurface bacteria during starvation and desiccation in a porous medium. Appl Environ Microbiol 60:3292–3299

  14. Martiny JB, Bohannan BJM, Brown JH, Colwell RK, Fuhrman JA, Green JL, Horner-Devine MC, Kane M, Krumins JA, Kuske CR, Morin PJ, Naeem S, Øvreås L, Reysenbach A, Smith VH, Staley JT (2006) Microbial biogeography: putting microorganisms on the map. Nature 4:102–112

  15. Petersen SO, Klug MJ (1994) Effects of sieving, storage and incubation temperature on the phospholipid fatty acid profile of a soil microbial community. Appl Environ Microbiol 60:2421–2430

  16. Pettersson M, Bååth E (2003) Temperature-dependent changes in the soil bacterial community in limed and unlimed soil. FEMS Microbiol Ecol 45:13–21

  17. Russell NJ, Fukunaga N (1990) A comparison of thermal adaptation of membrane lipids in psychrophilic and thermophilic bacteria. FEMS Microbiol Rev 75:171–182

  18. Schimel J, Balser TC, Wallenstein M (2007) Microbial stress-response physiology and its implications for ecosystem function. Ecology 88:1386–1394

  19. Staddon WJ, Trevors JT, Dunchesne LC, Colombo CA (1998) Soil microbial diversity and community structure across a climatic gradient in western Canada. Biodivers Conserv 7:1081–1092

  20. Vyverman W, Verleyen E, Sabbe K, Vanhoutte K, Sterken M, Hodgson DA, Mann DG, Juggins S, Vijver BVD, Jones V, Flower R, Roberts D, Chepurnov VA, Kilroy C, Vanormelingen P, Wever AD (2007) Historical processes constrain patterns in global diatom diversity. Ecology 88:1924–1931

  21. Wu Y, Ding N, Wang G, Xu J, Wu J, Brookes PC (2009) Effects of different soil weights, storage times and extraction methods on soil phospholipid fatty acid analyses. Geoderma 150:171–178

  22. Zogg G, Zak D, Ringelberg D, MacDonald N, Pregitzer K, White D (1997) Compositional and functional shifts in microbial communities due to soil warming. Soil Sci Soc Am J 61:475–481

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Acknowledgements

This work was jointly supported by the National Basic Research Program of China (2005CB121104), and the National Natural Science Foundation of China (40671092, 40425007). We would like to thank Richard H Bromilow for editing the manuscript.

Author information

Correspondence to Jianming Xu.

Additional information

Responsible editor: Chengrong Chen

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Wu, Y., Yu, X., Wang, H. et al. Does history matter? Temperature effects on soil microbial biomass and community structure based on the phospholipid fatty acid (PLFA) analysis. J Soils Sediments 10, 223–230 (2010). https://doi.org/10.1007/s11368-009-0118-5

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Keywords

  • History
  • Phospholipid fatty acid (PLFA) analysis
  • Soil microbial biomass
  • Soil microbial community
  • Temperature