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G-395A polymorphism in the promoter region of the KLOTHO gene associates with reduced cognitive impairment among the oldest old


This study aimed to examine the possible association between G-395A polymorphism in the promoter region of the KLOTHO gene and cognitive impairment among Chinese nonagenarians and centenarians. This study is a secondary analysis of the Project of Longevity and Aging in Dujiangyan (PLAD) study. Community-dwelling Chinese people aged 90 years or older were included. G-395A (rs1207568) genotyping in the promoter region of the KLOTHO gene was performed using the TaqMan allelic discrimination assay. Cognitive function was assessed with the mini-mental status examination (MMSE). A total of 706 participants (68.0 % female; mean age 93.5 ± 3.6 years) were included. The KLOTHO G-395A polymorphism genotype frequencies for the whole sample were 2.0 % AA, 30.3 % GA, and 67.7 % GG. The GG genotype frequencies for the cognitive impairment and control groups were 70.2 and 62.7 %, respectively. Cognitive impairment prevalence was significantly lower in the GA+AA group than in the GG genotype group (61.4 vs. 69.0 %, p = 0.044). GA+AA genotype subjects had a significantly lower risk of cognitive impairment (odds ratio 0.66; 95 % confidence interval 0.44 to 0.98) than GG genotype individuals after adjusting for age, gender, and other relevant risk factors. KLOTHO G-395A polymorphism associates with reduced cognitive impairment in a sample of Chinese nonagenarians and centenarians.

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The authors thank the staff of the Department of Geriatrics, Dujiangyan Hospital, and all participants and legal proxies for their great contributions.

Author information

Correspondence to Ming Yang.

Ethics declarations

Written informed consent was obtained from all subjects or their legal proxies. The Research Ethics Committee of Sichuan University approved the study protocol.

Conflict of interests

The authors declare that they have no competing interests.

Funding sources

This work was supported by the Project of Science and technology Bureau of Sichuan Province (2006Z09-006-4) and the Health Care Committee of Sichuan Province Health Department (2012-GANJIANBAN-7). The sponsor had no role in the design, methods, data collection, analysis, and preparation of this paper.

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Hao, Q., Ding, X., Gao, L. et al. G-395A polymorphism in the promoter region of the KLOTHO gene associates with reduced cognitive impairment among the oldest old. AGE 38, 7 (2016). https://doi.org/10.1007/s11357-015-9869-7

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  • KLOTHO gene
  • Cognitive impairment
  • Single nucleotide polymorphism