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Ambient temperature reduction extends lifespan via activating cellular degradation activity in an annual fish (Nothobranchius rachovii)

Abstract

Ambient temperature reduction (ATR) can extend the lifespan of organisms, but the underlying mechanism is poorly understood. In this study, cellular degradation activity was evaluated in the muscle of an annual fish (Nothobranchius rachovii) reared under high (30 °C), moderate (25 °C), and low (20 °C) ambient temperatures. The results showed the following: (i) the activity of the 20S proteasome and the expression of polyubiquitin aggregates increased with ATR, whereas 20S proteasome expression did not change; (ii) the expression of microtubule-associated protein 1 light chain 3-II (LC3-II) increased with ATR; (iii) the expression of lysosome-associated membrane protein type 2a (Lamp 2a) increased with ATR, whereas the expression of the 70-kD heat shock cognate protein (Hsc 70) decreased with ATR; (iv) lysosome activity increased with ATR, whereas the expression of lysosome-associated membrane protein type 1 (Lamp 1) did not change with ATR; and (v) the expression of molecular target of rapamycin (mTOR) and phosphorylated mTOR (p-mTOR) as well as the p-mTOR/mTOR ratio did not change with ATR. These findings indicate that ATR activates cellular degradation activity, constituting part of the mechanism underlying the longevity-promoting effects of ATR in N. rachovii.

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Abbreviations

ATR:

Ambient temperature reduction

LC3-II:

Microtubule-associated protein 1 light chain 3-II

Lamp 2a:

Lysosome-associated membrane protein type 2a

Hsc 70:

70-kD heat shock cognate protein

Lamp 1:

Lysosome-associated membrane protein type 1

mTOR:

Molecular target of rapamycin

p-mTOR:

Phosphorylated mTOR

ROS:

Reactive oxygen species

CAT:

Catalase

GPx:

Glutathione peroxidase

Mn-SOD:

Manganese-superoxide dismutase

CMA:

Chaperone-mediated autophagy

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Acknowledgments

This work was supported by grants NSC 99-2311-B-182-002-MY3, CMRPD1C0081, and CMRPD180353 from the Ministry of Science and Technology, Taiwan, and the Chang Gung Memorial Hospital, Linkou, Taiwan.

Conflict of interest

The authors have declared that there are no conflicts of interest in relation to the subject of this study.

Author contributions

CH conceived and designed the experiments, analyzed the data, and prepared the manuscript; CH and CL performed the experiments.

Author information

Correspondence to Chin-Yuan Hsu.

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Cite this article

Lu, C., Hsu, C. Ambient temperature reduction extends lifespan via activating cellular degradation activity in an annual fish (Nothobranchius rachovii). AGE 37, 33 (2015). https://doi.org/10.1007/s11357-015-9775-z

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Keywords

  • Aging
  • Temperature
  • Degradation
  • Autophagy
  • Fish