ACE I/D and ACTN3 R/X polymorphisms as potential factors in modulating exercise-related phenotypes in older women in response to a muscle power training stimuli
Genetic variation of the human ACE I/D and ACTN3 R577X polymorphisms subsequent to 12 weeks of high-speed power training on maximal strength (1RM) of the arm and leg muscles, muscle power performance (counter-movement jump), and functional capacity (sit-to-stand test) was examined in older Caucasian women [n = 139; mean age 65.5 (8.2) years; 67.0 (10.0) kg and 1.57 (0.06) m]. Chelex 100 was used for DNA extraction, and genotype was determined by PCR-RFLP methods. Muscular strength, power, and functional testing were conducted at baseline (T1) and after 12 weeks (T2) of high-speed power training. At baseline, the ACE I/D and ACTN3 R/X polymorphisms were not associated with muscle function or muscularity phenotypes in older Caucasian women. After the 12-week high-speed training program, subjects significantly increased their muscular and functional capacity performance (p < 0.05). For both polymorphisms, significant genotype-training interaction (p < 0.05) was found in all muscular performance indices, except for 1RM leg extension in the ACE I/D (p = 0.187). Analyses of the combined effects between genotypes showed significant differences in all parameters (p < 0.05) in response to high-speed power training between the power (ACTN3 RR + RX & ACE DD) versus “non-power” muscularity-oriented genotypes (ACTN3 XX & ACE II + ID)]. Our data suggest that the ACE and ACTN3 genotypes (single or combined) exert a significant influence in the muscle phenotypes of older Caucasian women in response to high-speed power training. Thus, the ACE I/D and ACTN3 R/X polymorphisms are likely factors in modulating exercise-related phenotypes in older women, particularly in response to a resistance training stimuli.
KeywordsGenotype Converting-enzyme genotype r577x genotypes Women Power
We would like to thank all the women who participated as subjects in this study as well as their mentor, Miguel Monteiro, Ph.D. of Polytechnic Institute of Bragança, for allowing them to do so. The study was developed according to a research fellowship with reference number SFRH/BD/47114/2008, funded by POPH–QREN—shared by the European Social Fund and the national funds of MCTES. The authors are also indebted to the Spanish Department of Health and Institute Carlos III of the Government of Spain [Spanish Net on Aging and frailty; (RETICEF)], Department of Health of the Government of Navarre, and Economy and Competitivity Department of the Government of Spain for financing this research with grants numbered RD06/013/1003, 87/2010, and DEP2011-24105, respectively The authors have no conflicts of interest that are directly relevant to the content of this article.
- Bouchard C, Rankinen T (2001) Individual differences in response to regular physical activity. Med Sci Sports Exerc 33:443–452Google Scholar
- Chanock SJ, Manolio T, Boehnke M, Boerwinkle E, Hunter DJ, Thomas G, Hirschhorn JN, Abecasis G, Altshuler D, Bailey-Wilson JE, Brooks LD, Cardon LR, Daly M, Donnelly P, Fraumeni JF Jr, Freimer NB, Gerhard DS, Gunter C, Guttmacher AE, Guyer MS, Harris EL, Hoh J, Hoover R, Kong CA, Merikangas KR, Morton CC, Palmer LJ, Phimister EG, Rice JP, Roberts J, Rotimi C, Tucker MA, Vogan KJ, Wacholder S, Wijsman EM, Winn DM, Collins FS (2007) Replicating genotype-phenotype associations. Nature 447(7145):655–660Google Scholar
- Costa AM, Silva AJ, Garrido ND, Louro H, Marinho DA, Marques MC et al (2009) Angiotensin-converting enzyme genotype affects skeletal muscle strength in elite athletes. J Sports Sci Med 8:410–418Google Scholar
- Garatachea N, Lucia A (2011) Genes and the ageing muscle: a review on genetic association studies. Age (Dordr). doi: 10.1007/s11357-011-9327-0
- Garatachea N, Fiuza-Luces C, Torres-Luque G, Yvert T, Santiago C, Gomez-Gallego F et al (2011) Single and combined influence of ACE and ACTN3 genotypes on muscle phenotypes in octogenarians. Eur J Appl Physiol. doi: 10.1007/s00421-011-2217-4
- Gineviciene V, Pranculis A, Jakaitiene A, Milasius K, Kucinskas V (2011) Genetic variation of the human ACE and ACTN3 genes and their association with functional muscle properties in Lithuanian elite athletes. Medicina (Kaunas) 47:284–290Google Scholar
- Gordon SE, Davis BS, Carlson CJ, Booth FW (2001) ANG II is required for optimal overload-induced skeletal muscle hypertrophy. Am J Physiol Endocrinol Metab 280:E150–E159Google Scholar
- Marsh AP, Miller ME, Rejeski WJ, Hutton SL, Kritchevsky SB (2009) Lower extremity muscle function after strength or power training in older adults. J Aging Phys Act 17:416–443Google Scholar
- Rodriguez-Romo G, Ruiz JR, Santiago C, Fiuza-Luces C, Gonzalez-Freire M, Gomez-Gallego F et al (2010) Does the ACE I/D polymorphism, alone or in combination with the ACTN3 R577X polymorphism, influence muscle power phenotypes in young, non-athletic adults? Eur J Appl Physiol 110:1099–1106CrossRefGoogle Scholar
- Scott RA, Irving R, Irwin L, Morrison E, Charlton V, Austin K et al (2010) ACTN3 and ACE genotypes in elite Jamaican and US sprinters. Med Sci Sports Exerc 42:107–112Google Scholar
- Williams AG, Day SH, Folland JP, Gohlke P, Dhamrait S, Montgomery HE (2005) Circulating angiotensin converting enzyme activity is correlated with muscle strength. Med Sci Sports Exerc 37:944–948Google Scholar
- Yu JG, Furst DO, Thornell LE (2003) The mode of myofibril remodelling in human skeletal muscle affected by DOMS induced by eccentric contractions. Histochem Cell Biol 119:383–393Google Scholar