Biomarkers of oxidative stress, antioxidant defence and inflammation are altered in the senescence-accelerated mouse prone 8
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In this study we compared biomarkers of oxidative stress, stress response, antioxidant defence and inflammation between mice (n = 10 per group, female, 7 months old) with an accelerated (SAMP8) and a normal ageing phenotype (SAMR1). As compared to SAMR1 mice, SAMP8 mice exhibited higher levels of lipid peroxides and protein carbonyls as well as a lower activity of the proteasomal subunit β-5. Furthermore, heme oxygenase-1 and paraoxonase-1 (PON-1) status was lower in SAMP8 mice indicating impaired stress response. Biomarkers of inflammation such as C-reactive protein and serum amyloid P were elevated in SAMP8 mice. Interestingly, impaired stress response and increased inflammation in SAMP8 mice were associated with elevated concentrations of ascorbic acid and α-tocopherol in the liver. An age-dependent increase in hepatic vitamin E and a decline in PON-1 gene expression were also observed in aged compared to young C57BL/6 mice.
KeywordsSAMP8 Accelerated ageing Stress response Proteasomal activity Ascorbic acid Tocopherol
BB is supported by TUBITAK (The Scientific and Technological Research Council of Turkey). JF is supported by grant no. FR 2478/4-1 from the German Research Foundation (DFG) and grant no. 0315679A from the German Federal Ministry of Education and Research (BMBF). GR is supported by the BMBF and the DFG Cluster of Excellence “Inflammation at Interfaces”.
- Chondrogianni N, Tzavelas C, Pemberton AJ, Nezis IP, Rivett AJ, Gonos ES (2005) Overexpression of proteasome β-5 subunit increases the amount of assembled proteasome and confers ameliorated response to oxidative stress and higher survival rates. J Biol Chem 280:11840–11850PubMedCrossRefGoogle Scholar
- Cuesta S, Kireev R, García C, Rancan L, Vara E, Tresguerres JA (2012) Melatonin can improve insulin resistance and aging-induced pancreas alterations in senescence-accelerated prone male mice (SAMP8). Age. doi: 10.1007/s11357-012-9397-7
- Packer L, Weber SU, Rimbach G (2001) Molecular aspects of α-tocotrienol antioxidant action and cell signalling. J Nutr 131:369–373Google Scholar
- Tsakiris S, Karikas GA, Parthimos T, Tsakiris T, Bakogiannis C, Schulpis KH (2009) Alpha-tocopherol supplementation prevents the exercise-induced reduction of serum paraoxonase 1/arylesterase activities in healthy individuals alpha-T vs PON 1/aryl activities. Eur J Clin Nutr 63:215–221PubMedCrossRefGoogle Scholar
- Wagner AE, Boesch-Saadatmandi C, Breckwoldt D, Schrader C, Schmelzer C, Döring F, Hashida K, Hori O, Matsugo S, Rimbach G (2011) Ascorbic acid partly antagonizes resveratrol mediated heme oxygenase-1 but not paraoxonase-1 induction in cultured hepatocytes—role of the redox-regulated transcription factor Nrf2. BMC Complement Altern Med 11:1–8PubMedCrossRefGoogle Scholar