, Volume 35, Issue 4, pp 1205–1217

Biomarkers of oxidative stress, antioxidant defence and inflammation are altered in the senescence-accelerated mouse prone 8

  • Banu Bayram
  • Sibylle Nikolai
  • Patricia Huebbe
  • Beraat Ozcelik
  • Stefanie Grimm
  • Tilman Grune
  • Jan Frank
  • Gerald Rimbach


In this study we compared biomarkers of oxidative stress, stress response, antioxidant defence and inflammation between mice (n = 10 per group, female, 7 months old) with an accelerated (SAMP8) and a normal ageing phenotype (SAMR1). As compared to SAMR1 mice, SAMP8 mice exhibited higher levels of lipid peroxides and protein carbonyls as well as a lower activity of the proteasomal subunit β-5. Furthermore, heme oxygenase-1 and paraoxonase-1 (PON-1) status was lower in SAMP8 mice indicating impaired stress response. Biomarkers of inflammation such as C-reactive protein and serum amyloid P were elevated in SAMP8 mice. Interestingly, impaired stress response and increased inflammation in SAMP8 mice were associated with elevated concentrations of ascorbic acid and α-tocopherol in the liver. An age-dependent increase in hepatic vitamin E and a decline in PON-1 gene expression were also observed in aged compared to young C57BL/6 mice.


SAMP8 Accelerated ageing Stress response Proteasomal activity Ascorbic acid Tocopherol 


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Copyright information

© American Aging Association 2012

Authors and Affiliations

  • Banu Bayram
    • 1
    • 2
  • Sibylle Nikolai
    • 1
  • Patricia Huebbe
    • 1
  • Beraat Ozcelik
    • 2
  • Stefanie Grimm
    • 3
  • Tilman Grune
    • 3
  • Jan Frank
    • 4
  • Gerald Rimbach
    • 1
  1. 1.Institute of Human Nutrition and Food ScienceChristian-Albrechts-UniversityKielGermany
  2. 2.Department of Food EngineeringIstanbul Technical UniversityIstanbulTurkey
  3. 3.Institute of Nutrition, Department of Nutritional ToxicologyFriedrich Schiller UniversityJenaGermany
  4. 4.Institute of Biological Chemistry and NutritionUniversity of HohenheimStuttgartGermany

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