AGE

, Volume 35, Issue 1, pp 159–170

Postprandial antioxidant gene expression is modified by Mediterranean diet supplemented with coenzyme Q10 in elderly men and women

  • Elena M. Yubero-Serrano
  • Lorena Gonzalez-Guardia
  • Oriol Rangel-Zuñiga
  • Nieves Delgado-Casado
  • Javier Delgado-Lista
  • Pablo Perez-Martinez
  • Antonio Garcia-Rios
  • Javier Caballero
  • Carmen Marin
  • Francisco M. Gutierrez-Mariscal
  • Francisco J. Tinahones
  • Jose M. Villalba
  • Isaac Tunez
  • Francisco Perez-Jimenez
  • Jose Lopez-Miranda
Article

Abstract

Postprandial oxidative stress is characterized by an increased susceptibility of the organism towards oxidative damage after consumption of a meal rich in lipids and/or carbohydrates. We have investigated whether the quality of dietary fat alters postprandial gene expression and protein levels involved in oxidative stress and whether the supplementation with coenzyme Q10 (CoQ) improves this situation in an elderly population. Twenty participants were randomized to receive three isocaloric diets each for 4 weeks: Mediterranean diet supplemented with CoQ (Med + CoQ diet), Mediterranean diet (Med diet), saturated fatty acid-rich diet (SFA diet). After 12-h fast, volunteers consumed a breakfast with a fat composition similar to that consumed in each of the diets. Nrf2, p22phox and p47phox, superoxide dismutase 1 and 2 (SOD1 and SOD2), glutathione peroxidase 1 (GPx1), thiorredoxin reductase (TrxR) gene expression and Kelch-like ECH associating protein 1 (Keap-1) and citoplasmic and nuclear Nrf2 protein levels were determined. Med and Med + CoQ diets induced lower Nrf2, p22phox, p47phox, SOD1, SOD2 and TrxR gene expression and higher cytoplasmic Nrf2 and Keap-1 protein levels compared to the SFA diet. Moreover, Med + CoQ diet produced lower postprandial Nrf2 gene expression and lower nuclear Nrf2 protein levels compared to the other diets and lower GPx1 gene expression than the SFA diet. Our results support the antioxidant effect of a Med diet and that exogenous CoQ supplementation has a protective effects against free radical overgeneration through the lowering of postprandial oxidative stress modifying the postprandial antioxidant protein levels and reducing the postprandial expression of antioxidant genes in peripheral blood mononuclear cells.

Keywords

CoQ10 Mediterranean diet Oxidative stress Gene expression 

Abbreviations

Apo

Apolipoprotein

CAT

Catalase

CoQ

Coenzyme Q10

GPx

Gluthatione peroxidase

H2O2

Hydrogen peroxide

Keap-1

Kelch-like ECH associating protein 1

Med diet

Mediterranean diet

Med + CoQ diet

Mediterranean supplemented with CoQ

MUFA

Monounsaturated fatty acid

PBMCs

Peripheral blood mononuclear cells

PUFA

Polyunsaturated fatty acid

ROS

Reactive oxygen species

SFA diet

Saturated fatty acid-rich diet

SOD

Superoxide dismutase

TG

Triacylglycerol

TrxR

Thiorredoxin reductase

Supplementary material

11357_2011_9331_MOESM1_ESM.doc (34 kb)
ESM 1DOC 33 kb
11357_2011_9331_MOESM2_ESM.doc (42 kb)
ESM 2DOC 41 kb
11357_2011_9331_MOESM3_ESM.doc (44 kb)
ESM 3DOC 44 kb
11357_2011_9331_MOESM4_ESM.doc (37 kb)
ESM 4DOC 37 kb

Copyright information

© American Aging Association 2011

Authors and Affiliations

  • Elena M. Yubero-Serrano
    • 1
  • Lorena Gonzalez-Guardia
    • 1
  • Oriol Rangel-Zuñiga
    • 1
  • Nieves Delgado-Casado
    • 1
  • Javier Delgado-Lista
    • 1
  • Pablo Perez-Martinez
    • 1
  • Antonio Garcia-Rios
    • 1
  • Javier Caballero
    • 2
  • Carmen Marin
    • 1
  • Francisco M. Gutierrez-Mariscal
    • 1
  • Francisco J. Tinahones
    • 1
    • 3
  • Jose M. Villalba
    • 4
  • Isaac Tunez
    • 5
  • Francisco Perez-Jimenez
    • 1
  • Jose Lopez-Miranda
    • 1
    • 6
  1. 1.Lipids and Atherosclerosis UnitIMIBIC/Reina Sofia University Hospital/University of Cordoba and CIBER Fisiopatologia Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos IIICórdobaSpain
  2. 2.Clinical Analysis ServiceReina Sofia University HospitalCordobaSpain
  3. 3.Hospital Virgen de la VictoriaMálaga and CIBER Fisiopatologia Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos IIICordobaSpain
  4. 4.Department of Cell Biology, Physiology and ImmunologyUniversity of CordobaCordobaSpain
  5. 5.Department of Biochemistry and Molecular Biology (IMIBIC), Faculty of MedicineUniversity of CordobaCordobaSpain
  6. 6.Lipids and Atherosclerosis Unit at Reina Sofia University HospitalCórdobaSpain

Personalised recommendations