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AGE

, Volume 34, Issue 2, pp 329–339 | Cite as

Age-related changes in the function of autophagy in rat kidneys

  • Jing Cui
  • Xue-Yuan BaiEmail author
  • Suozhu Shi
  • Shaoyuan Cui
  • Quan Hong
  • Guangyan Cai
  • Xiangmei ChenEmail author
Article

Abstract

Autophagy is a highly regulated intracellular process for the degradation of cytoplasmic components, especially protein aggregates and damaged organelles. It is essential for maintaining healthy cells. Impaired or deficient autophagy is believed to cause or contribute to aging and age-related disease. In this study, we investigated the effects of age on autophagy in the kidneys of 3-, 12-, and 24-month-old Fischer 344 rats. The results revealed that autophagy-related gene (Atg)7 was significantly downregulated in kidneys of increasing age. The protein expression level of the autophagy marker light chain 3/Atg8 exhibited a marked decline in aged kidneys. The levels of p62/SQSTM1 and polyubiquitin aggregates, representing the function of autophagy and proteasomal degradation, increased in older kidneys. The level of 8-hydroxydeoxyguanosine, a marker of mitochondrial DNA oxidative damage, was also increased in older kidneys. Analysis by transmission electron microscope demonstrated swelling and disintegration of cristae in the mitochondria of aged kidneys. These results suggest that autophagic function decreases with age in the kidneys of Fischer 344 rats, and autophagy may mediate the process of kidney aging, leading to the accumulation of damaged mitochondria.

Keywords

Autophagy Kidney Aging Mitochondria Oxidative damage 

Notes

Acknowledgments

This work was supported by a grant (no. 2007CB507403) from the National Basic Research Program of China to X.C., grants (no. 30870920, 30270505, and 30070288) from the National Natural Science Foundation of China to X.Y.B., and a grant (no. 2011CB964904) from the National Key Scientific Program of China to X.Y.B.

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Copyright information

© American Aging Association 2011

Authors and Affiliations

  1. 1.Department of Nephrology, Chinese PLA Institute and Key Lab of NephrologyChinese PLA General Hospital and Military Medical Postgraduate CollegeBeijingChina
  2. 2.Chinese PLA Institute of NephrologyChinese PLA General Hospital and Military Medical Postgraduate CollegeBeijingChina

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