Tissue-specific expression of receptor-interacting protein in aging mouse
- 69 Downloads
Receptor-interacting protein (RIP) is a well-characterized coregulator for nuclear receptors. Here, we report the expression of RIP as two isoforms with molecular weights of 140 kDa and 137 kDa in liver and kidney, but only as one isoform of 140 kDa in lung, adipose tissue, prostate and testis of mice. The levels of both the isoforms decreased in liver and kidney of old mice compared with adult mice. The expression of RIP140 in kidney was relatively lower in old males than females. In contrast, adipose tissue showed remarkably higher levels of RIP140 in old than adult mice of both sexes. Thus, the expression of RIP varied with the type of tissue, sex and age of mice, suggesting differences in its function as a coregulator.
KeywordsReceptor-interacting protein Coregulator Expression Aging Sex Mouse
The authors would like to thank Dr Ngan Vo for providing RIP140 antibody. Swati Ghosh is a recipient of Senior Research Fellowship from the University Grants Commission, India. This work was supported by grants from the Department of Biotechnology (BT/PR3593/Med/14/468/2003), Government of India to M.K.T.
- Augereau P, Badia E, Carascossa S, Castet A, Fritsch S, Harmand P, Jalaguier S, Cavaillès V (2006a) The nuclear receptor transcriptional coregulator RIP140. Nucl Recept Signal 4:1–4Google Scholar
- Powelka AM, Seth A, Virbasius JV, Kiskinis E, Nicoloro SM, Guilherme A, Tang X, Straubhaar J, Cherniack AD, Parker MG (2006) Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest 116:125–136PubMedCrossRefGoogle Scholar