AGE

, Volume 30, Issue 1, pp 31–42 | Cite as

Lysosome-targeted stress reveals increased stability of lipofuscin-containing lysosomes

  • Yuri Stroikin
  • Hanna Mild
  • Uno Johansson
  • Karin Roberg
  • Karin Öllinger
Article

Abstract

Cellular ageing is associated with accumulation of undegradable intralysosomal material, called lipofuscin. In order to accelerate the lipofuscin accumulation, confluent, growth-arrested human fibroblasts were cultured under hyperoxic conditions. To provide a better insight into the effects of lipofuscin on cellular functions, we compared lysosomal stability in control and lipofuscin-loaded human fibroblasts under conditions of lysosome-targeted stress induced by exposure to either the lysosomotropic detergent MSDH or the redox-cycling quinone naphthazarin. We show that lysosomal damage, assessed by acridine-orange relocation, translocation of cathepsin D to the cytosol, and alkalinization of lysosomes, is more pronounced in control than in lipofuscin-loaded fibroblasts. Finding that lysosomal integrity was less affected or even preserved in case of lipofuscin-loaded cells enables us to suggest that lipofuscin exerts lysosome-stabilizing properties.

Keywords

Alkalinization Autophagolysosomes Bafilomycin A1 Cathepsin D MSDH Naphthazarin quinone 

Notes

Acknowledgements

We thank Linda Vainikka for technical assistance. This study was financially supported by Lions Research Foundation and by grant from the Medical Branch of the Swedish Research Council (Vetenskapsrådet).

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Copyright information

© American Aging Association, Media, PA, USA 2008

Authors and Affiliations

  • Yuri Stroikin
    • 1
  • Hanna Mild
    • 1
  • Uno Johansson
    • 1
  • Karin Roberg
    • 2
  • Karin Öllinger
    • 1
  1. 1.Division of Experimental Pathology, Department of Neuroscience and Locomotion, Faculty of Health SciencesLinköping UniversityLinköpingSweden
  2. 2.Division of Oto-rhino-laryngology, Faculty of Health SciencesLinköping UniversityLinköpingSweden

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