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Effects of bone marrow-derived mesenchymal stem cells exposed to endocrine-disrupting chemicals on the differentiation of umbilical cord blood hematopoietic stem cells

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Abstract

Endocrine-disrupting chemicals (EDCs), a class of peripheral toxic substances, can cause many environmental and clinical side effects, particularly on the human body’s endocrine system. Bisphenol A (BPA) and diethylhexyl phthalate (DEHP) are two well-known EDCs in the medicine industry. However, there are no comprehensive studies on their effects on hematopoiesis. Hence, this study aimed to investigate the effect of these two aforementioned substances on the clonogenic capacity of umbilical cord blood hematopoietic stem cells (CB-HSCs). The HSCs which express CD34 + were isolated from umbilical cord blood by the magnetic-activated cell sorting (MACS) system. To investigate the effects of different optimized concentrations of BPA and DEHP, CB-CD34+ HSCs were exposed to EDCs in semisolid medium. For evaluation of coexposures, CB-CD34+ HSCs were cocultured with bone marrow-derived mesenchymal stem cells (BM-MSCs) in the presence of BPA and DEHP. Finally, the number and types of colonies were evaluated after 14 days. Statistical analysis was performed by GraphPad Prism through ANOVA. CB-HSC treated by BPA and DEHP showed a lower absolute colony count than the control group (P < 0.05). Decrease in clonogenic potential of HSCs was more significant in coculture condition by MSCs. In particular, there was a significant decrease in the BFU-E colonies in comedicated-derived fractions (P < 0.0001). In the presence of EDCs such as BPA and DEHP, the patterns of differentiation in CD34+ CB-HSCs changed from suppressed erythroid differentiation toward stimulated myelogenesis pathways.

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Please contact corresponding author (S.A.) for data requests.

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Abbreviations

BFU-E:

Burst-forming unit-erythroid

BPA:

Bisphenol A

BM-MSC:

Bone marrow-derived mesenchymal stem cells

CFU-E:

Colony-forming unit-erythroid

CFU-GM:

Colony-forming unit-granulocyte-monocyte

CFU-GEMM:

Colony-forming unit-granulocyte erythrocyte-monocyte-megakaryocyte

CFU-G:

Colony-forming unit-granulocyte

CFU-M:

Colony-forming unit-monocyte

CPDA1:

Citrate-phosphate-dextrose adenine

CB-HSC:

Cord blood hematopoietic stem cell

DEHP:

Diethylhexyl phthalate

DDT:

Dichlorodiphenyltrichloroethane

EDC:

Endocrine-disrupting chemical

FBS:

Fetal bovine serum

G-CSF:

Granulocyte colony-stimulating factor

MNC:

Mononuclear cell

PCBs:

Polychlorinated biphenyls

PBBs:

Polybrominated biphenyls

PVC:

Polyvinyl chloride

SCF:

Stem cell factor

TPO:

Thrombopoietin

UCB:

Umbilical cord blood

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Author notes

  1. Atefeh Soltani and Fatemeh Abbasnejadshani contributed equally to this file.

Authors and Affiliations

  1. Department of Medical Science, Islamic Azad University, Aligudarz, Lorestan, Iran

    Atefeh Soltani

  2. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Atefeh Soltani, Saeid Abroun, Fatemeh Abbasnejadshani & Mohammad Ali Gholampour

  3. Department of Laboratory Medicine, Khomein University of Medical Science, Khomein, Iran

    Atefeh Soltani, Saeid Abroun, Fatemeh Abbasnejadshani & Mohammad Ali Gholampour

Authors
  1. Atefeh Soltani
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  2. Saeid Abroun
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  3. Fatemeh Abbasnejadshani
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  4. Mohammad Ali Gholampour
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Contributions

All of the authors and coauthors who were listed on the title page greatly helped to accomplish this study with unsparing efforts. A.S. helped prepare the last version of the manuscript and perform a literature review. She also mainly contributed to conducting the experiment, data curation, data analysis, designation of images and tables, final arrangement of the data-making structure of the study, and visualization. S.A. mainly performed project supervision/correspondence, project administration, conceptualization, scientific consultations, and verification of the manuscript by definitive approval for submission. He also academically and grammatically performed peer revision and major conceptualization qualifying. F.A. equally helped A.S. for conducting experiment. MA.G equally helped A.S. for biostatistical analysis of data. S.A. and A.S. equally helped with critical revision of the manuscript on the important intellectual content according to the journal’s instructions, validation of data, and designation of methodological strategies. All of the authors and coauthors attest to the fact that they have approved the final version of the article, and they have received an electronic copy of the manuscript. Additionally, they attest to the validity and legitimacy of the data and agreed to submit this manuscript to this journal.

Corresponding author

Correspondence to Saeid Abroun.

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Ethical considerations

This experimental study accessed ethics approval from the Research Ethics Committee of Tarbiat Modares University (Tehran, Iran). The study was performed in accordance standard of Declaration of Helsinki.

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All healthy BM-MSC donors provided written informed consent and contributed to the study.

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Not applicable.

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The authors declare no competing interests.

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Responsible editor: Lotfi Aleya.

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Soltani, A., Abroun, S., Abbasnejadshani, F. et al. Effects of bone marrow-derived mesenchymal stem cells exposed to endocrine-disrupting chemicals on the differentiation of umbilical cord blood hematopoietic stem cells. Environ Sci Pollut Res 29, 39903–39913 (2022). https://doi.org/10.1007/s11356-021-17787-7

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