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Inhibition effects of some pesticides and heavy metals on carbonic anhydrase enzyme activity purified from horse mackerel (Trachurus trachurus) gill tissues

  • Cuneyt CaglayanEmail author
  • Parham Taslimi
  • Cebrahil Türk
  • İlhami Gulcin
  • Fatih Mehmet Kandemir
  • Yeliz Demir
  • Şükrü Beydemir
Research Article

Abstract

The gill tissue is the main site of metabolic enzymes or compensation, with the kidney tissue playing a supporting role. At the gill tissue, carbonic anhydrase enzymes (CAs) catalyze the hydration of CO2 to HCO3 and H+ for production to the H2O. In this work, the CA enzyme was purified from horse mackerel (Trachurus trachurus) gill with a specific activity of 21,381.42 EU/mg, purification fold of 150.61, total activity of 2347.68 EU/mL, and a yield of 16.13% using sepharose 4B-L-tyrosine-sulfanilamide affinity gel chromatography. For recording the enzyme purity, gel electrophoresis was performed, and single band was seen. The molecular weight of this enzyme was found approximately 35 kDa. Also, the inhibitory effects of different pesticides such as thiram, clofentezine, propineb, deltamethrin, azoxystrobin, and thiophanate and heavy metal ions such as Fe2+, Cu2+, Co2+, Pb2+ Hg2+, and As3+ on horse mackerel gill tissue CA enzyme activities were investigated. Our results indicated that these pesticides and metal ions showed inhibitory effects at low nanomolar and millimolar concentrations for fish gill CA enzymes, respectively.

Keywords

Carbonic anhydrase Horse mackerel Trachurus trachurus Gill Heavy metal Pesticides 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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© Springer-Verlag GmbH Germany, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of Veterinary MedicineBingol UniversityBingolTurkey
  2. 2.Department of Biotechnology, Faculty of ScienceBartin UniversityBartinTurkey
  3. 3.Department of Fisheries, Genç Vocational SchoolBingol UniversityBingolTurkey
  4. 4.Department of Chemistry, Faculty of ScienceAtatürk UniversityErzurumTurkey
  5. 5.Department of Biochemistry, Faculty of Veterinary MedicineAtatürk UniversityErzurumTurkey
  6. 6.Department of Pharmacy Services, Nihat Delibalta Göle Vocational High SchoolArdahan UniversityArdahanTurkey
  7. 7.Department of Biochemistry, Faculty of PharmacyAnadolu UniversityEskişehirTurkey

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